Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_012755650.1 RLEG_RS26495 dihydroxy-acid dehydratase
Query= reanno::BFirm:BPHYT_RS19730 (577 letters) >NCBI__GCF_000023185.1:WP_012755650.1 Length = 578 Score = 545 bits (1404), Expect = e-159 Identities = 276/573 (48%), Positives = 386/573 (67%), Gaps = 5/573 (0%) Query: 4 SNQTKKPLRSQAWFGLKDRDGFLHRSWMKNQGIPHDEFDGRPVIGICNTWSELTPCNAHF 63 + +T + LRS W D+ F HRS G +++ G+P+I + NTWS+ PC+ HF Sbjct: 3 ARKTYEQLRSARWMLPDDQRSFGHRSRTMQMGYAPEDWQGKPIIAVINTWSDAQPCHMHF 62 Query: 64 RELAEYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNPMDGVIL 123 RE AE+VK+G+ ++GG P+E P +SL E ++PT ML+RN+ +M+ EE +R +P+DG +L Sbjct: 63 RERAEWVKRGILQSGGFPMELPALSLSENFVKPTTMLYRNMLAMETEELLRSHPVDGAVL 122 Query: 124 LVGCDKTTPALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVRAGTMTQ 183 + GCDKTTP L+MGA S +P + + GPML G + GK +GSGT ++ +E RAGT+TQ Sbjct: 123 MGGCDKTTPGLVMGAVSMGIPFIYLPAGPMLRGNYAGKTLGSGTDGFKYWDERRAGTITQ 182 Query: 184 EEFTEAESCMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLAHLAGRR 243 EE+ E + RS GHCMTMGTASTM ++ E++G+ LP ++IPA DA Q ++ GRR Sbjct: 183 EEWQGIEGGIARSYGHCMTMGTASTMTAIAEAMGLTLPGASSIPAADANHQRMSAACGRR 242 Query: 244 IVDMVREDLTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLEDWE-LG 302 IVDMV EDLT D+I+T A +NA+ A G STNA++HLIA+A+R GV L L+D + +G Sbjct: 243 IVDMVWEDLTPDQIITPAAVDNAVTVAMATGCSTNAIIHLIAMARRAGVPLELDDLDRIG 302 Query: 303 SNVPCLVNLQPSGE-YLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDNVRNAA 361 P L N++PSG YLMEDF+YAGGL A++KQLG++ L A+TV GK L D + Sbjct: 303 RTTPVLANIRPSGSTYLMEDFFYAGGLRALMKQLGDK--LDPTAITVMGKPLVDGLDQVK 360 Query: 362 NYDEKVITTFAEPFKPKAGIAVLKGNLAPNGAVIKPSAATASLLKHRGRAVVFENIEELH 421 Y++ VI + P + +AVLKGNL P+GAVIKP+A +HRG A+V ++ E+ Sbjct: 361 IYNDDVIRPLSNPVYHEGSLAVLKGNLCPDGAVIKPAACDPKFHRHRGPALVADSYAEMK 420 Query: 422 AKIDDESLDIDEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQKGITDMVRISDGRMSGT 481 IDD + ++VL+ AGP+G PG E G +P+PK +L+ G+ DMVRISD RMSGT Sbjct: 421 KIIDDPDYPLTPDTVLVLRNAGPQGGPGMPEWGMIPMPKALLKLGLRDMVRISDARMSGT 480 Query: 482 AYGAVVLHVSPEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDEELARRRAAWQAPEAP- 540 ++GA VLHV+PE+ GGPLA ++TGDM+ELD+ R L++ V +EE+ RRAAW AP Sbjct: 481 SFGACVLHVAPESYVGGPLALLRTGDMVELDIPARSLNMLVAEEEITARRAAWVAPTRHY 540 Query: 541 KRGYYKLYVEHVLQADQGADLDFLVGSSGAPVP 573 +RGY ++ H+ QAD+G D DFL G P Sbjct: 541 ERGYGFMFSGHIEQADKGCDFDFLTTEFGGKTP 573 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 965 Number of extensions: 43 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 577 Length of database: 578 Length adjustment: 36 Effective length of query: 541 Effective length of database: 542 Effective search space: 293222 Effective search space used: 293222 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory