Align Xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate WP_012758573.1 RLEG_RS15645 dihydroxy-acid dehydratase
Query= reanno::pseudo5_N2C3_1:AO356_28760 (594 letters) >NCBI__GCF_000023185.1:WP_012758573.1 Length = 579 Score = 484 bits (1245), Expect = e-141 Identities = 261/570 (45%), Positives = 359/570 (62%), Gaps = 15/570 (2%) Query: 7 RRLRSEQWFNDPAHADMTALYVERYMNYGMTREELQSGRPIIGIAQTGSDLTPCNRHHLE 66 RRLRS++W+ + +Y ++ +L GRP+IGI T SD+TPCN H E Sbjct: 9 RRLRSQEWYGGTSR---DVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLRE 65 Query: 67 LAQRVKAGIRDAGGIPMEFPVHPIAEQSRRPTAALDRNLAYLGLVEILHGYPLDGVVLTT 126 LA++VKAG+ +AGG P+E PV +E + RPTA + RNLA L + E + G P+DG VL Sbjct: 66 LAEKVKAGVWEAGGFPLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLV 125 Query: 127 GCDKTTPACLMAAATTDLPAIVLSGGPMLDGHHKGELIGSGTVLWHARNLMAAGEIDYEG 186 GCDKTTP+ +M AA+ DLP+IV++GGPML+G+ +GE +GSGT LW ++ AGE+ Sbjct: 126 GCDKTTPSLIMGAASCDLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGEMTQAE 185 Query: 187 FMEMTTAASPSVGHCNTMGTALSMNALAEALGMSLPGCASIPAPYRERGQMAYATGKRIC 246 F+E + S S G CNTMGTA +M ++AEALGM+L G A+IP R MA TG+RI Sbjct: 186 FLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRIV 245 Query: 247 ELVLQDIRPSQIMTRQAFENAIAVASALGASSNCPPHLIAIARHMGVELSLDDWQRIGED 306 ++V D++PS+IMT+QAFENAI +A+G S+N HL+AIA +G++LSLDDW R G D Sbjct: 246 QMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRD 305 Query: 307 VPLLVNCMPAGKYLGEGFHRAGGVPSVMHELQKAGRLHEDCATVSGRTIGEIVSSSLTSN 366 VP +VN MP+GKYL E F AGG+P V+ L +AG LH+D TVSG T+ + V + N Sbjct: 306 VPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWN 365 Query: 367 ADVIHPFDTPLKHRAGFIVLSGNFF-DSAIMKMSVVGEAFRKTYLSEPGAENSFEARAIV 425 DVI P + L G +VL GN A++K S RA+V Sbjct: 366 EDVILPAEKALTASGGIVVLRGNLAPKGAVLKPSAASPHLL-----------VHRGRAVV 414 Query: 426 FEGPEDYHARIDDPALDIDERCILVIRGVGTVGYPGSAEVVNMAPPAALIKQGIDSLPCL 485 FE +DY A+I+D LDIDE CI+V++ G GYPG AEV NM P ++K+GI + + Sbjct: 415 FEDIDDYKAKINDENLDIDETCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRI 474 Query: 486 GDGRQSGTSASPSILNMSPEAAVGGGLALLQTNDRLKVDLNTRTVNLLIDDEEMARRRLE 545 D R SGT+ +L+ SPEAAVGG LA+++ D +++D+ R ++L I DEE+ARR E Sbjct: 475 SDARMSGTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAE 534 Query: 546 WTPNIPPSQTPWQELYRQLVGQLSTGGCLE 575 W PN + + L++Q V TG L+ Sbjct: 535 WQPNHDLPTSGYAFLHQQHVEGADTGADLD 564 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1013 Number of extensions: 62 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 594 Length of database: 579 Length adjustment: 37 Effective length of query: 557 Effective length of database: 542 Effective search space: 301894 Effective search space used: 301894 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory