Alignments for a candidate for fadA in Rhizobium leguminosarum WSM1325

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Rhizobium leguminosarum WSM1325

Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate WP_012763325.1 RLEG_RS27915 3-oxoadipyl-CoA thiolase

Query= metacyc::MONOMER-3207
         (400 letters)



>NCBI__GCF_000023185.1:WP_012763325.1
          Length = 401

 Score =  533 bits (1374), Expect = e-156
 Identities = 275/402 (68%), Positives = 325/402 (80%), Gaps = 3/402 (0%)

Query: 1   MRDVFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQA 60
           M + FICD IRTPIGRF G+L+ VRADDL A+PLKAL++ N AV W+ VD+V FGCANQA
Sbjct: 1   MTEAFICDYIRTPIGRFAGSLSQVRADDLGAIPLKALMQRNAAVDWEAVDDVIFGCANQA 60

Query: 61  GEDNRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESM 120
           GEDNRNVARM+ LLAGLP ++PG T+NRLC SGMDA+ TA RAI +GE EL IAGGVESM
Sbjct: 61  GEDNRNVARMSALLAGLPIAVPGTTINRLCGSGMDAVITAARAIRAGEAELMIAGGVESM 120

Query: 121 SRAPFVMGKAESGYSRNMKLEDTTIGWRFINPLMKSQYGVDSMPETADNVADDYQVSRAD 180
           SRAPFVM KAE+ +SR  ++ DTTIGWRF+NPLMK QYGVDSMPET +NVA+DY VSR D
Sbjct: 121 SRAPFVMPKAETAFSRAAEIHDTTIGWRFVNPLMKKQYGVDSMPETGENVAEDYHVSRED 180

Query: 181 QDAFALRSQQKAAAAQAAGFFAEEIVPVRIAHKKGE-TIVERDEHLRPETTLEALTKL-K 238
           QDAFA+RSQ KAAAAQA G  A+EI PV I+ +KG+  IV++DEH R  TT+E L KL  
Sbjct: 181 QDAFAVRSQAKAAAAQANGRLAKEITPVTISQRKGDPVIVDKDEHPR-ATTIETLAKLAT 239

Query: 239 PVNGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASGGVAPRVMGIGP 298
           P      TVTAGNASGVNDGAAALI+AS  A +K+GLTP AR+LG A+  V PRVMG+GP
Sbjct: 240 PFKKEGGTVTAGNASGVNDGAAALIVASEAAARKYGLTPIARILGGAAAAVPPRVMGVGP 299

Query: 299 VPAVRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGVADDAPQVNPNGGAIALGHPL 358
           +PA RKL  RLG+    FDVIELNEAFASQGLAVLR LG+ADD  +VN NGGAIALGHPL
Sbjct: 300 IPASRKLMARLGMTADQFDVIELNEAFASQGLAVLRALGIADDDARVNRNGGAIALGHPL 359

Query: 359 GMSGARLVLTALHQLEKSGGRKGLATMCVGVGQGLALAIERV 400
           GMSGAR+  TA  +L ++GG+  L+TMC+GVGQG+A+A++RV
Sbjct: 360 GMSGARITGTAALELLQTGGQYSLSTMCIGVGQGIAIALKRV 401


Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 518
Number of extensions: 21
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 401
Length adjustment: 31
Effective length of query: 369
Effective length of database: 370
Effective search space:   136530
Effective search space used:   136530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_012763325.1 RLEG_RS27915 (3-oxoadipyl-CoA thiolase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.13360.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.8e-138  446.2   7.2   5.5e-138  446.0   7.2    1.0  1  lcl|NCBI__GCF_000023185.1:WP_012763325.1  RLEG_RS27915 3-oxoadipyl-CoA thi


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000023185.1:WP_012763325.1  RLEG_RS27915 3-oxoadipyl-CoA thiolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  446.0   7.2  5.5e-138  5.5e-138       1     384 [.       6     398 ..       6     399 .. 0.95

  Alignments for each domain:
  == domain 1  score: 446.0 bits;  conditional E-value: 5.5e-138
                                 TIGR01930   1 ivdavRtpigklggslkelsaedLlaavikellera.gldpekidevilGnvlqageq.aniaReaala 67 
                                               i d++Rtpig++ gsl++++a+dL+a  +k+l++r+  +d e++d+vi+G++ qage+  n+aR+ al 
  lcl|NCBI__GCF_000023185.1:WP_012763325.1   6 ICDYIRTPIGRFAGSLSQVRADDLGAIPLKALMQRNaAVDWEAVDDVIFGCANQAGEDnRNVARMSALL 74 
                                               7899********************************9*********************9********** PP

                                 TIGR01930  68 aglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakl 136
                                               aglp  vp++t+nr+C+Sg++Av++aa++i+aGea++++aGGvEsmSr+p++++++   e++  ++a+ 
  lcl|NCBI__GCF_000023185.1:WP_012763325.1  75 AGLPIAVPGTTINRLCGSGMDAVITAARAIRAGEAELMIAGGVESMSRAPFVMPKA---ETAFSRAAEI 140
                                               ***************************************************99996...5555567777 PP

                                 TIGR01930 137 edqllkdl........vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpve 197
                                               +d+++           ++   sm et en+a++y++sRe+qD++a+rS+ kaa+A+++g++++ei+pv+
  lcl|NCBI__GCF_000023185.1:WP_012763325.1 141 HDTTIGWRfvnplmkkQYGVDSMPETGENVAEDYHVSREDQDAFAVRSQAKAAAAQANGRLAKEITPVT 209
                                               7744443378999998777889*********************************************** PP

                                 TIGR01930 198 vkgk...kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgl 263
                                               + ++   +++v+kDe++r  tt+e+LakL + fk++ g tvtAgN+s++nDGAaal+++se++a+++gl
  lcl|NCBI__GCF_000023185.1:WP_012763325.1 210 ISQRkgdPVIVDKDEHPR-ATTIETLAKLATPFKKEGG-TVTAGNASGVNDGAAALIVASEAAARKYGL 276
                                               *9999*99**********.899************9877.7***************************** PP

                                 TIGR01930 264 tplarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsld.lek 331
                                               tp+ari + a a+v+p++mg+gp+pA++k++++ g++++++d++E+nEAFA+q lav ++lg  d +++
  lcl|NCBI__GCF_000023185.1:WP_012763325.1 277 TPIARILGGAAAAVPPRVMGVGPIPASRKLMARLGMTADQFDVIELNEAFASQGLAVLRALGIADdDAR 345
                                               **************************************************************86679** PP

                                 TIGR01930 332 vNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvil 384
                                               vN nGGAiAlGHPlG+sGari+ t + eL + g++y l t+C+g GqG+A+ l
  lcl|NCBI__GCF_000023185.1:WP_012763325.1 346 VNRNGGAIALGHPLGMSGARITGTAALELLQTGGQYSLSTMCIGVGQGIAIAL 398
                                               **************************************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (401 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 7.52
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources