Align Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized)
to candidate WP_012854485.1 TCUR_RS20495 ATP-grasp domain-containing protein
Query= SwissProt::I3R7G3 (601 letters) >NCBI__GCF_000024385.1:WP_012854485.1 Length = 584 Score = 525 bits (1352), Expect = e-153 Identities = 292/599 (48%), Positives = 384/599 (64%), Gaps = 28/599 (4%) Query: 4 KVLVANRGEIAVRVMRACEELGVRTVAVYSEADKHGGHVRYADEAYNIGPARAADSYLDH 63 KVL+ANRGEIAVR+ RAC + G+ +VAVY+E D HVR ADEAY +G AA+SYLD Sbjct: 3 KVLIANRGEIAVRIARACRDAGLASVAVYAEPDLDALHVRIADEAYALGGQTAAESYLDI 62 Query: 64 ESVIEAARKADADAIHPGYGFLAENAEFARKVEDSEFTWVGPSADAMERLGEKTKARSLM 123 E +++ A + ADA+HPGYGFLAENA+FAR V D+ W+GP +A+ LG+K KAR + Sbjct: 63 EKILKIAADSGADAVHPGYGFLAENADFARAVIDAGLIWIGPPPEAITALGDKVKARHIA 122 Query: 124 QDADVPVVPGTTEPADSAEDVKAVADDYGYPVAIKAEGGGGGRGLKVVHSEDEVDGQFET 183 Q P+V GT +P E+V A A+++G P+AIKA GGGGRGLKV + +EV +E+ Sbjct: 123 QKVGAPLVAGTKDPVSGVEEVVAFAEEHGLPIAIKAAYGGGGRGLKVARTLEEVPELYES 182 Query: 184 AKREGEAYFDNASVYVEKYLEAPRHIEVQILADEHGNVRHLGERDCSLQRRHQKVIEEAP 243 A RE F +VE+YL+ PRH+E Q LAD+HGNV + RDCSLQRRHQK++EEAP Sbjct: 183 AVREAIGAFGRGECFVERYLDKPRHVETQCLADKHGNVVVVSTRDCSLQRRHQKLVEEAP 242 Query: 244 SPALSEDLRERIGEAARRGVRAAEYTNAGTVEFLV-EDGEFYFMEVNTRIQVEHTVTEEV 302 +P L+++ E + +++ ++ A Y AGT EFLV +DG F+EVNTR+QVEH VTEEV Sbjct: 243 APFLTDEQIELLYSSSKAILKEAGYVGAGTCEFLVGQDGTISFLEVNTRLQVEHPVTEEV 302 Query: 303 TGLDVVKWQLRVAAGEELDFSQDDVEIEGHSMEFRINAEAPEKEFAPATGTLSTYDPPGG 362 TG+D+V+ R+AAGEEL + DD + GHS+EFRINAE + F PA GTL T+ PP G Sbjct: 303 TGIDLVREMFRIAAGEELGY--DDPPVRGHSIEFRINAEDAGRNFLPAVGTLRTWRPPSG 360 Query: 363 IGIRMDDAVRQGDEIGGDYDSMIAKLIVTGSDREEVLVRAERALNEFDIEGLRTVIPFHR 422 G+R+D +G I +DS+IAKLIVTG+ RE+ + RA RAL EF ++G TV+PFHR Sbjct: 361 PGVRLDSGYEEGQTIPQAFDSLIAKLIVTGATREQAIERARRALAEFTVDGTPTVLPFHR 420 Query: 423 LMLTDEAFREGSHTTKYLDE--VLDPERIEAAVERWSPEAVAGDEEEGEVTER-TFTVEV 479 +L D A Y+ E + IE + A EE E ER TVEV Sbjct: 421 AVLEDPA---------YVGEPFAVHTRWIETEFDNTIAPYQADPAEESEAAERERITVEV 471 Query: 480 NGKRFEVSLEERGAPAIPLG-GASAAASASKPSGPRKRREESDEGGQQVIEGDGESVAAE 538 GKR EV+L P G GAS A+A K + P +RR GG G+S+ + Sbjct: 472 GGKRLEVTL--------PAGLGASGGAAAGK-AAPARRRGGRKGGG---ASAGGDSLVSP 519 Query: 539 MQGTILAVEVDEGDDVEPGDTVCILEAMKMENDVVAERGGTVSQVLVGEGDSVDMGDVL 597 MQGTI+ V V+EG V GDTV +LEAMKME + A + GTV+ + GD+V G V+ Sbjct: 520 MQGTIVKVVVEEGQTVAEGDTVVVLEAMKMEQPLTAHKAGTVTGLSAKVGDTVTNGAVI 578 Lambda K H 0.312 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 930 Number of extensions: 48 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 2 Length of query: 601 Length of database: 584 Length adjustment: 37 Effective length of query: 564 Effective length of database: 547 Effective search space: 308508 Effective search space used: 308508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory