Alignments for a candidate for tpi in Klebsiella variicola At-22

GapMind for catabolism of small carbon sources

Alignments for a candidate for tpi in Klebsiella variicola At-22

Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate WP_012968564.1 KVAR_RS16155 triose-phosphate isomerase

Query= BRENDA::Q7X216
         (265 letters)



>NCBI__GCF_000025465.1:WP_012968564.1
          Length = 264

 Score =  520 bits (1338), Expect = e-152
 Identities = 255/264 (96%), Positives = 257/264 (97%)

Query: 2   MTPIWLGTSWKMNKPLSQAMAWCETLAARMPEGCHPAIQPFVIPSFTAIQPVSHFLQTHQ 61
           MTPIWLGTSWKMNKPLSQAMAWCETLAARMPEGCHPAIQPFVIP FTAIQPVSHFLQ HQ
Sbjct: 1   MTPIWLGTSWKMNKPLSQAMAWCETLAARMPEGCHPAIQPFVIPPFTAIQPVSHFLQMHQ 60

Query: 62  LPLLTGAQNMHEADQGAWTGEISAAMLAETGATLVELGHSERRAAFNESDAAINRKVHSA 121
           LPLLTGAQNMHEA QGAWTGEISAAMLAETGATLVELGHSERRAAFNESDAAINRKVHSA
Sbjct: 61  LPLLTGAQNMHEAGQGAWTGEISAAMLAETGATLVELGHSERRAAFNESDAAINRKVHSA 120

Query: 122 LGHGLRPLICIGDSAEEKRWQVSRESVVRQMKIALYGLSHQQALRTLIAYEPVWAIGEHG 181
           LGHGLRPLICIGDSAEEKRWQVSRESVVRQMKIALYGLSHQQAL+ LIAYEPVWAIGEHG
Sbjct: 121 LGHGLRPLICIGDSAEEKRWQVSRESVVRQMKIALYGLSHQQALQILIAYEPVWAIGEHG 180

Query: 182 TPASPQEAGVIHQALRQALCERFGHETGTRIPLLYGGXVTLQNAVELLRQQEINGLFIGR 241
           TPASPQEAGVIHQALR+ALCERFGHETG RIPLLYGG VTLQNAVELLRQ EINGLFIGR
Sbjct: 181 TPASPQEAGVIHQALREALCERFGHETGIRIPLLYGGSVTLQNAVELLRQPEINGLFIGR 240

Query: 242 AAWDAQGYCDIVQRVTQEFILQAQ 265
           AAWDAQGYCDIVQRVTQEFILQAQ
Sbjct: 241 AAWDAQGYCDIVQRVTQEFILQAQ 264


Lambda     K      H
   0.321    0.133    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 439
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 265
Length of database: 264
Length adjustment: 25
Effective length of query: 240
Effective length of database: 239
Effective search space:    57360
Effective search space used:    57360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate WP_012968564.1 KVAR_RS16155 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00419.hmm
# target sequence database:        /tmp/gapView.29469.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    1.5e-42  132.0   0.0    1.7e-42  131.8   0.0    1.0  1  lcl|NCBI__GCF_000025465.1:WP_012968564.1  KVAR_RS16155 triose-phosphate is


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000025465.1:WP_012968564.1  KVAR_RS16155 triose-phosphate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  131.8   0.0   1.7e-42   1.7e-42       4     224 ..       8     242 ..       5     246 .. 0.90

  Alignments for each domain:
  == domain 1  score: 131.8 bits;  conditional E-value: 1.7e-42
                                 TIGR00419   4 infKlnesvgkvelevaklaeevaseagveva..vappfvdldvvkdeve.seiqv..aAqnvdavksG 67 
                                                 +K+n  +++      +la+ + + +  ++   v ppf  ++ v++ ++  ++++  +Aqn++   +G
  lcl|NCBI__GCF_000025465.1:WP_012968564.1   8 TSWKMNKPLSQAMAWCETLAARMPEGCHPAIQpfVIPPFTAIQPVSHFLQmHQLPLltGAQNMHEAGQG 76 
                                               579*******************99998766644499***********999656665337********** PP

                                 TIGR00419  68 aftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartin 136
                                               a+tGeisA+ml+++Ga  v +gHsErR+ ++e+d  i+ kv  +   gl++++C+g++ ee+    + +
  lcl|NCBI__GCF_000025465.1:WP_012968564.1  77 AWTGEISAAMLAETGATLVELGHSERRAAFNESDAAINRKVHSALGHGLRPLICIGDSAEEKRWQVSRE 145
                                               **************************************************************9999999 PP

                                 TIGR00419 137 nvattaaaaA.......lepdvvAvEPveliG.tGkpvskAeaevveksvrdhlkk.vskevaesvrvl 196
                                                v ++ ++a          + ++A+EPv++iG  G p+s+ ea v+++ +r+ l +   +e    + +l
  lcl|NCBI__GCF_000025465.1:WP_012968564.1 146 SVVRQMKIALyglshqqALQILIAYEPVWAIGeHGTPASPQEAGVIHQALREALCErFGHETGIRIPLL 214
                                               9999998887676655545679**********55******************9988799********** PP

                                 TIGR00419 197 yGasvtaaedaelaaqldvdGvLlasav 224
                                               yG+svt ++++el  q++++G+ ++ a 
  lcl|NCBI__GCF_000025465.1:WP_012968564.1 215 YGGSVTLQNAVELLRQPEINGLFIGRAA 242
                                               ************************9886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (264 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.91
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources