Alignments for a candidate for glk in Allochromatium vinosum DSM 180

GapMind for catabolism of small carbon sources

Alignments for a candidate for glk in Allochromatium vinosum DSM 180

Align Glucokinase; EC 2.7.1.2; Glucose kinase (uncharacterized)
to candidate WP_012970537.1 ALVIN_RS06545 glucokinase

Query= curated2:B2J224
         (341 letters)



>NCBI__GCF_000025485.1:WP_012970537.1
          Length = 326

 Score =  233 bits (595), Expect = 4e-66
 Identities = 144/332 (43%), Positives = 185/332 (55%), Gaps = 14/332 (4%)

Query: 4   LLAGDIGGTKTILQLVETSDSQGLHTIYQESYHSADFPDLVPIVQQFLIKANTPIPEKAC 63
           LL GDIGGTKT L L ET D   +       Y SA F  L  IVQ++L++        A 
Sbjct: 3   LLVGDIGGTKTALGLAET-DGGSVRLGETRRYPSASFGSLDQIVQRYLLETGATC-RFAV 60

Query: 64  FAIAGPIVKNTAKLTNLAWFLDTERLQQELGIPHIYLINDFAAVGYGISGLQKQDLHPLQ 123
           FA+AGPI     + TNL W LD E L+Q LG+  + L+ND  AV +G+  L   DL  L 
Sbjct: 61  FAVAGPIHDRRCETTNLPWVLDAEALEQSLGLTCVELLNDLEAVAWGVPILSSDDLAELH 120

Query: 124 VGKPQPETPIGIIGAGTGLGQGFLIKQGNNYQVFPSEGGHADFAPRNEIEFQLLKYLLDK 183
            G P  +    ++ AGTGLGQ  L   G  +  F +EGGH+DFAP +++EF LL YL  K
Sbjct: 121 PGDPCSQGNACVVAAGTGLGQAGLFWDGLRHHAFATEGGHSDFAPADDLEFALLTYL--K 178

Query: 184 HDIQRISVERVVSGMGIVAIYQFLRDRKFAAESPDIAQIVRTWEQEAGQEEKSVDPGAAI 243
               R+S ERVVSG GIV ++ FLR    + ++PD  Q V          +   D  A I
Sbjct: 179 SRFGRVSWERVVSGPGIVNLFDFLRFH-HSVQAPDWLQAV---------IDTGGDTAATI 228

Query: 244 GTAALEKRDRLSEQTLQLFIEAYGAEAGNLALKLLPYGGLYIAGGIAPKILPLIQNSGFL 303
             AA + R  L  +TL LF+  YG EAGN ALK +  GG+Y+ GGIA K L  ++  GFL
Sbjct: 229 AQAAADDRCPLCRETLNLFMRLYGREAGNAALKHMALGGVYLGGGIALKNLACLRRGGFL 288

Query: 304 LNFTQKGRMRPLLEEIPVYIILNPQVGLIGAA 335
             F  KGRM  L+  +PV +IL P   L+GAA
Sbjct: 289 EGFFDKGRMGSLMRRMPVRVILQPNTPLLGAA 320


Lambda     K      H
   0.320    0.140    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 282
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 341
Length of database: 326
Length adjustment: 28
Effective length of query: 313
Effective length of database: 298
Effective search space:    93274
Effective search space used:    93274
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_012970537.1 ALVIN_RS06545 (glucokinase)
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.18470.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.8e-87  278.9   0.0    3.2e-87  278.7   0.0    1.0  1  lcl|NCBI__GCF_000025485.1:WP_012970537.1  ALVIN_RS06545 glucokinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000025485.1:WP_012970537.1  ALVIN_RS06545 glucokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  278.7   0.0   3.2e-87   3.2e-87       1     315 []       4     320 ..       4     320 .. 0.93

  Alignments for each domain:
  == domain 1  score: 278.7 bits;  conditional E-value: 3.2e-87
                                 TIGR00749   1 lvgdiGGtnarlalvevapgeieqv..ktyssedfpsleavvrvyleeakvelkdpikgcfaiatPiig 67 
                                               lvgdiGGt++ l+l e   g ++    + y s  f sl ++v+ yl e+  + +    + fa+a+Pi +
  lcl|NCBI__GCF_000025485.1:WP_012970537.1   4 LVGDIGGTKTALGLAETDGGSVRLGetRRYPSASFGSLDQIVQRYLLETGATCRF---AVFAVAGPIHD 69 
                                               89*************99999999773366999*****************999998...*********** PP

                                 TIGR00749  68 dfvrltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaaiailGaGtG 136
                                                 ++ tnl W l  e l+q+l+l+ +el+nd+ ava++++ l ++dl+ l   ++  ++   ++ aGtG
  lcl|NCBI__GCF_000025485.1:WP_012970537.1  70 RRCETTNLPWVLDAEALEQSLGLTCVELLNDLEAVAWGVPILSSDDLAELHPGDPCSQGNACVVAAGTG 138
                                               ***************************************************99999999999******* PP

                                 TIGR00749 137 lGvatliqqsdgrykvlageGghvdfaPrseleillleylrkkygrvsaervlsGsGlvliyealskrk 205
                                               lG+a l      r++++a+eGgh dfaP ++le+ ll yl++++grvs erv+sG+G+v+++++l+ ++
  lcl|NCBI__GCF_000025485.1:WP_012970537.1 139 LGQAGLFW-DGLRHHAFATEGGHSDFAPADDLEFALLTYLKSRFGRVSWERVVSGPGIVNLFDFLRFHH 206
                                               *******9.456999***************************************************999 PP

                                 TIGR00749 206 gerevsklskeelkekd....iseaalegsdvlarralelflsilGalagnlalklgarGGvyvaGGiv 270
                                               + +    l+    +  d    i++aa ++ ++l+r++l+lf+ ++G++agn alk  a GGvy+ GGi+
  lcl|NCBI__GCF_000025485.1:WP_012970537.1 207 SVQAPDWLQAVIDTGGDtaatIAQAAADDRCPLCRETLNLFMRLYGREAGNAALKHMALGGVYLGGGIA 275
                                               888855554444333334445************************************************ PP

                                 TIGR00749 271 PrfiellkkssfraafedkGrlkellasiPvqvvlkkkvGllGag 315
                                                + +  l++++f ++f dkGr+++l+  +Pv+v+l+ ++ llGa+
  lcl|NCBI__GCF_000025485.1:WP_012970537.1 276 LKNLACLRRGGFLEGFFDKGRMGSLMRRMPVRVILQPNTPLLGAA 320
                                               *******************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (326 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.37
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources