Alignments for a candidate for cimA in Azospirillum sp. B510

Align (R)-citramalate synthase (EC 2.3.3.21) (characterized)
to candidate WP_012974983.1 AZL_RS12885 citramalate synthase

Query= BRENDA::Q74C76
         (528 letters)



>NCBI__GCF_000010725.1:WP_012974983.1
          Length = 533

 Score =  494 bits (1272), Expect = e-144
 Identities = 265/526 (50%), Positives = 348/526 (66%), Gaps = 10/526 (1%)

Query: 4   VKLYDTTLRDGTQAEDISFLVEDKIRIAHKLDEIGIHYIEGGWPGSNPKDVAFFKDIKKE 63
           + LYDTTLRDG Q +D+ F V DKI IA  LD +GI YIEGGWPG+NP D  FF +  + 
Sbjct: 6   IYLYDTTLRDGAQTQDVDFSVADKIAIARDLDRLGIDYIEGGWPGANPTDDRFFAEAPE- 64

Query: 64  KLSQAKIAAFGSTRRAKVTPDKDHNLKTLIQAEPDVCTIFGKTWDFHVHEALRISLEENL 123
            LS+A   AFG TRR   +   D  L  L Q+      + GK+WDFHV  AL I   ENL
Sbjct: 65  -LSRAIFTAFGMTRRPGRSAANDPQLTALAQSRAKAVCMVGKSWDFHVDVALNIPRAENL 123

Query: 124 ELIFDSLEYLKANVPEVFYDAEHFFDGYKANPDYAIKTLKAAQDAKADCIVLCDTNGGTM 183
           +LI DS+  L+A   E  +DAEHFFDGYK NP YA+  +KAAQDA A  IVLCDTNGGT+
Sbjct: 124 DLIRDSIAALRAAKGEALFDAEHFFDGYKRNPAYAVSCIKAAQDAGARWIVLCDTNGGTL 183

Query: 184 PFELVEIIREVRKHITAP---LGIHTHNDSECAVANSLHAVSEGIVQVQGTINGFGERCG 240
           P E+  I+REV +    P   LGIH HND+E AVANSL AV  G   VQGTING GERCG
Sbjct: 184 PHEIDAIVREVVETHGIPGDQLGIHCHNDTENAVANSLAAVRAGARMVQGTINGLGERCG 243

Query: 241 NANLCSIIPALKLKMKREC-IGDDQLRKLRDLSRFVYELANLSPNKHQAYVGNSAFAHKG 299
           NANL S+IP L LKM  E  I  + L  L  LSR   +  N +PN+H  YVG SAFAHKG
Sbjct: 244 NANLVSLIPTLMLKMGYETGIRREDLPLLTQLSRAFDDRLNRAPNRHAPYVGASAFAHKG 303

Query: 300 GVHVSAIQRHPETYEHLRPELVGNMTRVLVSDLSGRSNILAKAEEFNIKMDSKDPVTLEI 359
           G+HVSA+++ P +YEH+ P+ +GN  ++++SD +GRSN++A+  +  + +D+KD     +
Sbjct: 304 GLHVSAVEKDPSSYEHVAPDAIGNRRQIVMSDQAGRSNLIARLRDIGMAVDAKDERLTGL 363

Query: 360 LENIKEMENRGYQFEGAEASFELLMKRALGTHRKFFSVIGFRVIDEKRHE---DQKPLSE 416
           L+ +K  ++ GY ++ AEASFELL++R LG   +FF ++ F V DE+R+    +    SE
Sbjct: 364 LDLVKRRDSDGYAYDTAEASFELLVRRELGEVPRFFELLRFHVTDERRYNAVGEMVVESE 423

Query: 417 ATIMVKVGGKIEHTAAEGNGPVNALDNALRKALEKFYPRLKEVKLLDYKVRVLPAGQGTA 476
           A + V++G ++    A+GNGPV+ALD A+RKALE  YP L +V L DY+VR+L A  GT 
Sbjct: 424 AVVRVRIGDELRMEVADGNGPVHALDVAMRKALEPAYPALAKVSLSDYRVRILAAKDGTG 483

Query: 477 SSIRVLIESGDKE-SRWGTVGVSENIVDASYQALLDSVEYKLHKSE 521
           +  RVLI S   + + W T+GVS NI++AS +AL+DS  Y L K +
Sbjct: 484 AMPRVLIRSARTDGTEWSTLGVSTNIIEASMEALVDSYTYALMKGD 529


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 669
Number of extensions: 21
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 528
Length of database: 533
Length adjustment: 35
Effective length of query: 493
Effective length of database: 498
Effective search space:   245514
Effective search space used:   245514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_012974983.1 AZL_RS12885 (citramalate synthase)
to HMM TIGR00977 (cimA: citramalate synthase (EC 2.3.1.182))

Software error:

../bin/blast/fastacmd -i /tmp/list.18862.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.18862.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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