Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_012976491.1 AZL_RS21100 3-hydroxyacyl-CoA dehydrogenase
Query= reanno::BFirm:BPHYT_RS13545 (706 letters) >NCBI__GCF_000010725.1:WP_012976491.1 Length = 728 Score = 572 bits (1473), Expect = e-167 Identities = 330/703 (46%), Positives = 432/703 (61%), Gaps = 19/703 (2%) Query: 18 VLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAGADIREFGKPP 77 V ++T+DH PVNAL+ +R L AA+ AA D +V A+++ GAGR F G DI EF P Sbjct: 23 VAVITLDHPPVNALALALRTRLGAALHAALRDVSVRAIVLAGAGRGFCGGGDITEFDLPA 82 Query: 78 V--PPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLGLPEVQLGL 135 V P+ + + IE KPVVAA+HG ALGGGLE+ALA H R+A ++GLPEV LGL Sbjct: 83 VCQEPTPATLFSLIENSPKPVVAALHGMALGGGLELALACHARVAEARTQVGLPEVHLGL 142 Query: 136 LPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRLGSSDDILAE---GLAYV 192 +PGAGGTQR PRL+G + AL+LI+ GR A+ GL DRL + A +A Sbjct: 143 VPGAGGTQRLPRLVGFELALNLIVQGRTQPAQSLRDSGLFDRLTDGSPVEAAVEVAMALA 202 Query: 193 HELLAAHAPVRRTRDAAALSDRAASLAAV-ATARAETAKKSRGLFSPLKIVDAVEAAIEQ 251 EL +A R AL+ R A+ A A ARA + RGL +P+ VD VE A Sbjct: 203 GELAVPNAADGMLRRTGALTPRLANAQAFFAFARATVQSRFRGLPAPVACVDCVEKAATL 262 Query: 252 PFDEGLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAA----KPRTLNTIGVVG 307 PF EGL E F + + AGL HAF AER +A + R KPR + T+ V+G Sbjct: 263 PFHEGLAFEHDCFTRLRGTAEFAGLRHAFLAER---RAADIRGLPDGIKPRPVKTVAVIG 319 Query: 308 GGTMGAGIAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALMSR 367 GTMG GIA+++ +AGL VT+IER+ +L RG A I + + + KGRL+AE+ A + Sbjct: 320 AGTMGGGIAMSLANAGLAVTLIEREQEALDRGLAIIRQTCESALRKGRLTAEEAAGRLDL 379 Query: 368 WSGSTSYDALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALASSV 427 G YD + ADLVIEAVFEDL VK+ VF ++D V +AG VLATNTS LD+D +A Sbjct: 380 IKGCVGYDPVRQADLVIEAVFEDLEVKRQVFEQIDAVARAGTVLATNTSMLDLDRIAGFT 439 Query: 428 SRPADVIGLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGN 487 RP DV+GLHFFSPA++MKLLEVV + DV+ATA LA+++ KT V +GVC+GFIGN Sbjct: 440 RRPQDVLGLHFFSPAHVMKLLEVVRGAATAPDVLATAIALARRIGKTSVVSGVCEGFIGN 499 Query: 488 RVLAVYRSAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAATRN 547 R+L Y A ++++GA P+Q+D A+ +G MGPF+V DLAG D+G R + A R Sbjct: 500 RILQPYLYQAGRLLDEGALPHQVDQAIERWGMAMGPFRVSDLAGNDLGAGIRAQVMANR- 558 Query: 548 PAARYVQIADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPRSF 607 P + D + G FGQKSGRG+Y Y R P EV A + AE R G+ R+ Sbjct: 559 PELAFSGSFDAVVAMGRFGQKSGRGWYDYSASQRGPQPSMEVNAAVVAESGRLGLRRRTI 618 Query: 608 TDEEIIRRYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYADMVGLPK 667 DEEII R + A++NEGA V+ + IA R D+DV + GYGFPR+RGGPM AD GL Sbjct: 619 ADEEIIDRLLLALVNEGAKVLGDGIAQRASDIDVVYTAGYGFPRWRGGPMFAADRRGLAD 678 Query: 668 ILADIREFAK-----EDPLFWKPSPLLIELVERGADFASLNQS 705 IL +R FA+ +D FW+P+PLL L +RG F + S Sbjct: 679 ILGTMRRFAEAAPDPQDASFWQPAPLLRRLADRGRSFGTFVDS 721 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1236 Number of extensions: 61 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 728 Length adjustment: 40 Effective length of query: 666 Effective length of database: 688 Effective search space: 458208 Effective search space used: 458208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory