Align Probable 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 (characterized)
to candidate WP_012977172.1 AZL_RS24815 3-hydroxyacyl-CoA dehydrogenase
Query= SwissProt::O32178 (789 letters) >NCBI__GCF_000010725.1:WP_012977172.1 Length = 779 Score = 481 bits (1239), Expect = e-140 Identities = 304/801 (37%), Positives = 434/801 (54%), Gaps = 40/801 (4%) Query: 5 IRKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNDLTKEEEKKGLTKDSSEVRSRLSRQ 64 I +AAV+G+GVMG+GIAAH AN GIP +LLDI K+ ++ RS ++R Sbjct: 3 IERAAVIGAGVMGAGIAAHFANAGIPCVLLDIP-------------AKEGAD-RSAIARG 48 Query: 65 AMKKLLKQKPAPLTSAKNTSYITPGNLEDDAEKLKEADWIIEVVVENLEVKKKIFALVDE 124 A+ K+LK PAP KN ITPGNLEDD L + DWI+E VVEN +K ++ +D Sbjct: 49 AVAKMLKTDPAPFMHPKNAKLITPGNLEDDLSLLADVDWIVEAVVENPAIKADLYKRIDP 108 Query: 125 HRKTGSIVSSNTSGISVQEMAEGRSDDFKAHFLGTHFFNPARYLKLLEIIPIKETDPDIL 184 RK GS+VSSNTS I + + EG+ + F FL THFFNP RY++LLEI+ T PD + Sbjct: 109 VRKAGSVVSSNTSTIPLAVLTEGQGEQFAKDFLITHFFNPPRYMRLLEIVGGAHTRPDAM 168 Query: 185 KFMTAFGENVLGKGVVTAKDTPNFIANRIGTYGLLVTVQEMLKGGYQVGEVDSITGPLIG 244 + + LGKGVV KDTP FIANRIG + V + G V E D++ G +G Sbjct: 169 AAIADVCDRRLGKGVVHCKDTPGFIANRIGVLWIQAAVNAAVDLGLTVEEADAVGGRPMG 228 Query: 245 RPKSATFRTLDVVGLDTFAHVARNVYDKADGDE--KEVFRIPSFMNDMLEKGWIGSKAGQ 302 PK+ F +D+VGLD H+A+++ + + R + M+ +G+ G K Sbjct: 229 IPKTGIFGLMDLVGLDLMPHIAKSMSATLPAGDAYRGQMREHPVITKMIAEGYTGRKGKG 288 Query: 303 GFYKKE----GKTIYELDPVTLTYGERTKMKSPALEAAKQAKGTKAKMKALIYSDDRAGR 358 GFY+ GK ++ T Y K + ++ AA + ++ L D+ GR Sbjct: 289 GFYRINKTGGGKVKESVNLRTGDYAPSEKARLDSVSAAGR------DLRKLCEHPDKGGR 342 Query: 359 LLWNITSQTLLYSAELLGEIADDIHAIDQAMKWGFGWELGPFEMWDAIGLKQSAEKLEQL 418 + +QTL Y+A L+ EIAD I A+D+ M+ G+ W+ GPFE+ D +G + AE Sbjct: 343 FARRVLAQTLAYAASLVPEIADSIVAVDEGMRLGYNWKQGPFELIDRLGTEWFAELCRAE 402 Query: 419 GADMPGWIKEMLDKGNETFYIKENGTVFYY-DRGEYRAVKENKKRIHLQALKETKG-VIA 476 G +P ++ FY E G + + G Y V + L +K G + Sbjct: 403 GIPVPALVE---TAAGRPFYRVEGGKLQHLTTAGVYDTVVRPDGVLLLSDIKRAAGKPVW 459 Query: 477 KNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGLE---ETERNYKGLVIGNQGKNF 533 KN ASL D+GD V +EF SK NA+ DI+ K + + + ++K LVI N+ NF Sbjct: 460 KNGSASLWDIGDGVLCVEFTSKMNAVDADIMAAYEKAMRLIGDGKGDWKALVIHNEADNF 519 Query: 534 CVGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKPVVAAPFGMTLGGGTEACLP 593 VGANL + L + + +++ ++ Q T +KY+ PVVAAP GM LGGG E L Sbjct: 520 SVGANLGLALFALNIGLWPQIEEMVEGGQRTYRALKYAPFPVVAAPSGMALGGGCEILLH 579 Query: 594 AARIQAASEAYMGLVESGVGLIPGGGGNKELYINHL---RRGHDPMNAAMKTFETIAMAK 650 + +QA +E Y+GLVE GVGLIP GG E+ H + PM K FE I+ A Sbjct: 580 SDHVQAHAETYVGLVEVGVGLIPAWGGCTEMLARHQANPKAPRGPMPGTAKAFEIISTAT 639 Query: 651 VSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWRPPVKEKVKV-PGETGY 709 V+ SA EA+E+ + TD I++N+D LL DAK A L + PP K V G + Sbjct: 640 VAKSAAEAKELLYFRATDGITMNRDRLLADAKAKALEL-AANYTPPEKTTSYVLAGPSAK 698 Query: 710 AALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGG-KVPFGTEVDEEYLLEIEREAFLSLS 768 AA+ L E L G ++ HD + L+ V++GG + V E+++L +EREAF+ L Sbjct: 699 AAMELAVEGFALQGKVTPHDKLVCAALSEVLSGGEEADISKPVGEDHILRLEREAFMGLV 758 Query: 769 GEAKSQARMQHMLVKGKPLRN 789 + R++HML+ GKPLRN Sbjct: 759 RTGGTIDRIEHMLLTGKPLRN 779 Lambda K H 0.316 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1412 Number of extensions: 64 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 789 Length of database: 779 Length adjustment: 41 Effective length of query: 748 Effective length of database: 738 Effective search space: 552024 Effective search space used: 552024 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory