Align proline dehydrogenase (EC 1.5.5.2) (characterized)
to candidate WP_012977293.1 AZL_RS25435 sarcosine oxidase subunit alpha
Query= BRENDA::Q76M76 (483 letters) >NCBI__GCF_000010725.1:WP_012977293.1 Length = 1000 Score = 145 bits (365), Expect = 8e-39 Identities = 138/490 (28%), Positives = 205/490 (41%), Gaps = 87/490 (17%) Query: 15 RGRKVTIHFEGQPVEAYEGETIAMALHAAGIRVLSHSAEKHRPRGLFCAIGKCSSCLVKV 74 RGR+V F+G EG+T+A AL A G+ ++ S + HRPRG+ A + + LV Sbjct: 14 RGRQVRFSFDGVDYTGCEGDTLASALLANGVHLVGRSFKYHRPRGILTAGSEEPNALVGS 73 Query: 75 NG-----VPNVRSCITLVEEGMKVEMQRGKETLPKG------------------------ 105 N PN R+ + V EG+K E Q +L + Sbjct: 74 NRGNGRFEPNTRATVLEVFEGLKTESQNRWPSLTRDIGAVNDALYRLFSAGFYYKTFMWP 133 Query: 106 ------------------AKPPAWKDAPRYKA-----DVVVIGGGPAGLMAAIHAADAGA 142 K P D Y + DV+V+GGG AGL AA+ A GA Sbjct: 134 RSFWDKLYEPVIRNAAGLGKAPTAPDPDSYASRFAHCDVLVVGGGAAGLAAALEAGRGGA 193 Query: 143 SVILIDENPMLGGQLVKQTHKFFGKREQFAGVRGVKIAEILGEEVKKRGNIEVFLETSAV 202 SVIL DE LGG L+ + + G E + N+ V T+A+ Sbjct: 194 SVILCDEQNELGGSLLSEP------AAEINGQLAWDWLEDTNAALAAMPNVTVLPRTTAI 247 Query: 203 GVFHEGEEKLVAAVRKN----------KELLEFLGKTLVVATGAMEKMIPFENNDLPGIY 252 G +H+ L + + + + + K +V+A GA+EK + F ND PG+ Sbjct: 248 GYYHQNFVGLCQRLTDHLAIVPDGAPRERMWKVRAKRVVLAQGALEKPLVFAGNDRPGVM 307 Query: 253 GAGAIQTLMNTYGVKPGDRVLIVGAGNVGLILAYQLIQAGVEVKAIVEAMPKVGGYFVHA 312 AGA +T +N YGVK G+RV++V + + A+ L +AGVEV AIV+ V A Sbjct: 308 LAGAARTYLNRYGVKVGNRVVVVTSHDSAWHAAFDLAKAGVEVAAIVDIRGSVPSQLAAA 367 Query: 313 AKVRRLGVPILTRHTILRAEGKDRVERAVIAQLDENWRPVPGTEKVFEVDTIALAVGLRP 372 A R + L T+ +G+ R+ + + V G E + D + ++ G P Sbjct: 368 ADALR--IKTLIGWTVSDTKGRLRISSVRVNPVTGG--KVGGAETI-ACDALLMSGGWTP 422 Query: 373 SIELLHQAGCQVKFVRE----LSGHVAVRDGRMETTVQGIFVAGDSAGIEEATTAMLEGK 428 S+ L + + + L GH G+ E +AG G A +G Sbjct: 423 SVHLFSHTQGSLTWNEDGQMFLPGH-----GKEECR-----LAGAGMGRFGLKAAFEDGA 472 Query: 429 IAGIAAALKA 438 AG AAA A Sbjct: 473 AAGAAAAAAA 482 Lambda K H 0.319 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1062 Number of extensions: 54 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 483 Length of database: 1000 Length adjustment: 39 Effective length of query: 444 Effective length of database: 961 Effective search space: 426684 Effective search space used: 426684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory