Protein WP_012978494.1 in Azospirillum lipoferum B510
Annotation: NCBI__GCF_000010725.1:WP_012978494.1
Length: 514 amino acids
Source: GCF_000010725.1 in NCBI
Candidate for 27 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-galactose catabolism | ytfR | hi | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized) | 64% | 100% | 628.6 | Fructose import ATP-binding protein FruK; EC 7.5.2.- | 48% | 459.5 |
| L-arabinose catabolism | araVsh | med | ABC transporter related (characterized, see rationale) | 54% | 99% | 505.4 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-fructose catabolism | fruK | med | Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized) | 48% | 98% | 459.5 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| sucrose catabolism | fruK | med | Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized) | 48% | 98% | 459.5 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-mannose catabolism | HSERO_RS03640 | med | Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) | 41% | 96% | 386 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| myo-inositol catabolism | iatA | med | Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) | 43% | 97% | 384.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-cellobiose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-glucose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| lactose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-maltose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| sucrose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| trehalose catabolism | mglA | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-xylose catabolism | xylG | med | Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) | 40% | 99% | 369.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-galactose catabolism | BPHYT_RS16930 | med | Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale) | 41% | 96% | 369.4 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-xylose catabolism | xylK_Tm | med | Ribose import ATP-binding protein RbsA 1; EC 7.5.2.7 (characterized, see rationale) | 41% | 95% | 368.2 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-ribose catabolism | rbsA | med | ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 (characterized) | 40% | 99% | 361.7 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-fructose catabolism | frcA | med | ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) | 42% | 94% | 359 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| sucrose catabolism | frcA | med | ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) | 42% | 94% | 359 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| L-fucose catabolism | HSERO_RS05250 | lo | Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) | 39% | 98% | 390.6 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| myo-inositol catabolism | PS417_11890 | lo | m-Inositol ABC transporter, ATPase component (itaA) (characterized) | 39% | 97% | 354 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| L-arabinose catabolism | araG | lo | L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 (characterized) | 39% | 99% | 343.6 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| 2'-deoxyinosine catabolism | H281DRAFT_01113 | lo | deoxynucleoside transporter, ATPase component (characterized) | 37% | 99% | 323.2 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| L-fucose catabolism | BPHYT_RS34245 | lo | ABC transporter related; Flags: Precursor (characterized, see rationale) | 37% | 95% | 283.5 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| L-rhamnose catabolism | BPHYT_RS34245 | lo | ABC transporter related; Flags: Precursor (characterized, see rationale) | 37% | 95% | 283.5 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-mannose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 40% | 98% | 163.3 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| D-ribose catabolism | frcA | lo | Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized) | 40% | 98% | 163.3 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
| L-arabinose catabolism | xylGsa | lo | Xylose/arabinose import ATP-binding protein XylG; EC 7.5.2.13 (characterized, see rationale) | 34% | 100% | 161.8 | galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) | 64% | 628.6 |
Sequence Analysis Tools
View WP_012978494.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MTASTAPPSAPLLAIRGLSKAFLGVQALDGVDFTLRHGEIHALLGENGAGKSTLIKTLTG
VYQRDGGTVALEGREIAPRGVEEAQRLHIGTVYQEVNLLPNLSVAENLFLGRQPMRFGLV
DRGAMRRRARAVLQPYGLSVDVTAPLGRFSVATQQIIAIARAVDMSAKVLILDEPTASLD
AQEVAVLFKVMRTLRSHGIGIVFVTHFLDQVYEVCDRITVLRNGRLVGERRTAELPRLDL
VAMMLGRELEAAAHRIDLGPEEIDTRPPLARFKAFGKARSVEPFDLDIRPGEVVALAGLL
GSGRTETVRLVFGMDRADSGEAVVDGRPARLKGPRDAVRLGFGFCPEDRKKEGIVGELSV
RENIILALQARQGWLKPIPRRRQEEIADRFIRLLDIRTPDAERPIQLLSGGNQQKALLAR
WLATEPRLLILDEPTRGIDVGAHAEIIRLIERLCADGMALLVVSSELEEIVAYSRRVVVL
RDRRHVAELRGGEVSVERIVAAIASETPNREVRQ
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory