Alignments for a candidate for Psest_0084 in Denitrovibrio acetiphilus DSM 12809

GapMind for catabolism of small carbon sources

Alignments for a candidate for Psest_0084 in Denitrovibrio acetiphilus DSM 12809

Align alpha-ketoglutarate TRAP transporter, 4TM/12TM components (characterized)
to candidate WP_013010475.1 DACET_RS05940 C4-dicarboxylate ABC transporter permease

Query= reanno::psRCH2:GFF84
         (674 letters)



>NCBI__GCF_000025725.1:WP_013010475.1
          Length = 632

 Score =  342 bits (878), Expect = 2e-98
 Identities = 212/620 (34%), Positives = 340/620 (54%), Gaps = 18/620 (2%)

Query: 7   LHASPSEWPRALFYVALLFSI-YQIVTAAFHPVSSQVLRAGHVGFLLLLVFLCYPARGNG 65
           ++A   ++ + +F +A +F + +    A   PVS++  +  +V     L+FL Y ++   
Sbjct: 1   MYADLKKFEKIIFDIAAVFLVLFYSYAALIKPVSTEYHKGIYVFITYFLIFLVYKSKN-- 58

Query: 66  KPFQPVAWLLGLAGFATFFYQWYFEADLIQRSGDMTTADMVVGLTLIVLVFEAARRVMGI 125
           K  + + ++L LA      Y   F  DL  R+G  T  D  + +  +++  E ARR +G 
Sbjct: 59  KWLRIIDYILILAACVPVLYFMIFFVDLNYRAGAETIMDQWMSVVGVLMGIEVARRAVGS 118

Query: 126 ALPIICALFLAYGLLGEYLPGDLAHRG-YYLDQIVNQLSFGTEGLYGTPTYVSATYIFLF 184
              ++    + YG+ G+ +P    H G  +LD I   +    +G++G    V ATY+ LF
Sbjct: 119 VFVLLGGAMILYGVFGDKVPDLFQHSGGNFLDTISVAIFLTNDGVFGIMADVLATYVLLF 178

Query: 185 ILFGSFLEQAGMIKLFTDFAMGLFGHKLGGPAKVSVVSSALMGTITGSGVANVVTTGQFT 244
           +LFG+FLE++G  K F D  +   GHK GGPAKV+V++SA+ G+I+GS +AN V+TG FT
Sbjct: 179 VLFGAFLEKSGAQKFFIDLPLAAVGHKTGGPAKVAVIASAIFGSISGSAIANTVSTGAFT 238

Query: 245 IPLMKRFGYRPAFAGGVEATSSMGSQIMPPVMGAVAFIMAETINVPFVEIAKAALIPALL 304
           IPLMK+ G++P  AG VE  +S+G   MPP+MGA  FIMAE   VP+  I   A+ PAL+
Sbjct: 239 IPLMKKAGFKPHVAGAVEPAASIGGMFMPPIMGAGGFIMAELTEVPYQTIMLVAIFPALM 298

Query: 305 YFGSVFWMVHLEAKRAGLKGLPKDECPSAMAAVKERWYLLIPLVVLVWLLFSGRTPMFAG 364
           YF SVF MVH EAK   ++G  +    S     KE W+ ++PLV++  L+  G++P ++ 
Sbjct: 299 YFFSVFVMVHYEAKLHNIRG--EKSAVSPKQIFKEGWHYVMPLVLITILMIMGKSPGYSA 356

Query: 365 TIGLALTAIVILGSAIILKVSNFALRIAFWIALGLLCAGFFQLGIGVIFGVIAALVAVCW 424
            +G+ L+ ++I  +     VS F +     + LG+L A   + G   +  + A L  +  
Sbjct: 357 VVGM-LSCVIITWTRGSKYVSIFTI-----VLLGVLFADSVK-GFDSVNEMSAGLSTLVT 409

Query: 425 F-IKGGRDTLVICLHALVEGARH----AVPVGIACALVGVIIGVVSLTGVASTFAGYILA 479
           F     R  L I L   +E +R+    ++ +G    ++G+II +++ +G+  TF+  I+ 
Sbjct: 410 FGFAALRGELRINLKEFIEASRNGTESSLKIGATVGVIGIIISMLTYSGLTLTFSSIIIH 469

Query: 480 VGENNLFLSLLLTMLTCLVLGMGIPTIPNYIITSSIAAPALLDLGVPLIVSHMFVFYFGI 539
           +    L+L++LL  L  LVLGMG+P    Y+IT+ +A PAL +LGV  I +HM V++   
Sbjct: 470 LAAGKLWLTILLIALASLVLGMGVPVTAAYLITAVVAVPALTELGVSPIAAHMVVYWLSQ 529

Query: 540 MADLTPPVALACFAAAPIAKERGLKISMWAIRIAIAGFIVPFMAVYNPALMMQGGDWGAT 599
            +++TPPV +A FA A IAK      +  A + A   ++ PF+  Y PA  M+    G+ 
Sbjct: 530 DSNITPPVCIAAFAGATIAKANMWLTAFNAFKFAKFLYLGPFLFAYVPAFSMRHYLPGSD 589

Query: 600 LYMLFKAAFAVGLWGAVFTG 619
            Y  F        + AV  G
Sbjct: 590 SYEWFSPTTITLTFIAVIIG 609


Lambda     K      H
   0.330    0.143    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1155
Number of extensions: 66
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 674
Length of database: 632
Length adjustment: 38
Effective length of query: 636
Effective length of database: 594
Effective search space:   377784
Effective search space used:   377784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources