GapMind for Amino acid biosynthesis

 

Alignments for a candidate for leuD in Kyrpidia tusciae DSM 2912

Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33 (characterized, see rationale)
to candidate WP_013076329.1 BTUS_RS11950 3-isopropylmalate dehydratase small subunit

Query= uniprot:Q845W4
         (216 letters)



>NCBI__GCF_000092905.1:WP_013076329.1
          Length = 204

 Score =  229 bits (585), Expect = 2e-65
 Identities = 120/206 (58%), Positives = 143/206 (69%), Gaps = 11/206 (5%)

Query: 1   MEKFTVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK 60
           ME F  H G+V PLDR NVDTD IIPKQFLK I+RTGFG   F +WR+ + G P      
Sbjct: 1   MEPFVRHEGLVLPLDRVNVDTDQIIPKQFLKRIQRTGFGQFLFYDWRFREDGSP------ 54

Query: 61  RPLNPDFVLNQPRYQGASILVTRKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC 120
              +P F+LN P Y+ ASIL+ R NFGCGSSREHAPWALQ YGFR IIAPSFADIF+NNC
Sbjct: 55  ---DPKFILNHPEYREASILLARDNFGCGSSREHAPWALQDYGFRVIIAPSFADIFYNNC 111

Query: 121 FKNGLLPIVLTEQQVDHLINETVAFNG-YQLTIDLEAQVVRTPDGRDYPFEITAFRKYCL 179
           FKNGLLP+VL  + V+HL +E     G Y+LT+DLE+Q V    G    FEI  FR+  L
Sbjct: 112 FKNGLLPLVLPGETVNHLFDEAGRIPGRYRLTVDLESQEVWDNRGWQTRFEIDPFRRESL 171

Query: 180 LNGFDDIGLTLRHADKIRQFEAERLA 205
           L G DDIG+TL   D+I  +E +R+A
Sbjct: 172 LKGLDDIGVTLEKEDRIAGYE-QRMA 196


Lambda     K      H
   0.322    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 188
Number of extensions: 5
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 204
Length adjustment: 21
Effective length of query: 195
Effective length of database: 183
Effective search space:    35685
Effective search space used:    35685
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate WP_013076329.1 BTUS_RS11950 (3-isopropylmalate dehydratase small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))

Software error:

../bin/blast/fastacmd -i /tmp/list.25529.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.25529.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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