Align fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized)
to candidate WP_013418243.1 RVAN_RS02765 3-hydroxyacyl-CoA dehydrogenase
Query= CharProtDB::CH_003230 (714 letters) >NCBI__GCF_000166055.1:WP_013418243.1 Length = 724 Score = 425 bits (1092), Expect = e-123 Identities = 272/722 (37%), Positives = 385/722 (53%), Gaps = 26/722 (3%) Query: 5 SAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDN 64 S+ + V D IA +TID PG+ MN + EF ++ I++ + ++G+V S K + Sbjct: 2 SSISYQVDADGIATLTIDQPGKSMNVISPEFTEELSEAIERAAADSSVKGIVITSGK-SS 60 Query: 65 FIAGADI----NMIGNCKTAQEAEALARQGQ--QLMAEIHALPIQVIAAIHGACLGGGLE 118 F+AGAD+ MI + + A AEAL + +++ + + V AI+G LGGG E Sbjct: 61 FVAGADLIGMSGMIEHARNAPAAEALKVSSRLTEVLRRLETIGKPVATAINGTALGGGFE 120 Query: 119 LALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQ 178 +ALA H RV DD +G PEVQ+GLLPG+GGTQR+PRLIG+ + ++L GK L +Q Sbjct: 121 IALATHYRVAADDESIKIGQPEVQVGLLPGAGGTQRIPRLIGIMASAPLLLEGKHLSPQQ 180 Query: 179 ALKLGLVDDVVPHSILL-EAAVELAKKERPSSRPLPVRERILAGPLG-----RALLFKMV 232 A GL+ DVVP + LL +A L + S +P R + G G A LF Sbjct: 181 AKSSGLIHDVVPAAELLAKAKAWLLSPQAKSVQPWDERGFKVPGGAGGMHPAAAQLFMAA 240 Query: 233 GKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQAL-RSIFF- 290 T KT GNYPA + IL V G G EA+ F L P + A+ R++F Sbjct: 241 NAMTRDKTLGNYPAAQAILSAVYEGTQVPIDLGLKIEAKYFLSLLRGPVAPAMIRTLFVN 300 Query: 291 ---ASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINH 347 A V++ G P A +G+LG G+MG IAYV A GI V + D + + Sbjct: 301 KGRADKLVRRPQG--VPKAKFKKIGMLGAGMMGAAIAYVAASN-GIEVVLLDRDVEAAER 357 Query: 348 ALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMV 407 Y+ +E V RR ++ LA I T DY A D IIEAVFE+ +K + Sbjct: 358 GKGYARKLVEKAVSRRKTTEADGQGLLARIHATADYADLADVDYIIEAVFEDRAIKADVT 417 Query: 408 AEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSA 467 +VE IF SNTS++PI +A + RPE+ IG+HFFSP EKMPLVEII TS Sbjct: 418 KKVEAVIRPDVIFGSNTSTIPITSLAEASERPEKFIGIHFFSPAEKMPLVEIIRGKKTSD 477 Query: 468 QTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFG 527 + +A T+ + KTPIVV D GF+ +R YI E MLT+G ++ A G Sbjct: 478 EALAVTLDFVQAIRKTPIVVNDGRGFFTSRFCMNYIGEGEIMLTEGIPAALVENAGKLAG 537 Query: 528 FPVGPIQLLDEVGIDTGTKIIPVLEAAYGERF--SAPANVVSSI-LNDDRKGRKNGRGFY 584 PVGP+ L DE ID G KI E G+ + SA V+ ++ + + R GRKNG+GFY Sbjct: 538 MPVGPLALADETAIDLGWKIHKAAETDLGDAYTPSAAIGVMEAMYVKNGRAGRKNGKGFY 597 Query: 585 LYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRD 644 Y + G K+ ++P+ I + +R + +AVR +++ V+ D Sbjct: 598 EYPEGGPKTLWAGLADLFPVKPESEWPSID--DLKKRLLFAQALDAVRTMEDGVLTDPED 655 Query: 645 GDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGES 704 GD+G +FG+GF P+ GGP +D +G VA +RLA +YG RF+P + L +M A G + Sbjct: 656 GDVGGIFGLGFAPYTGGPLSMLDGIGIANAVAECERLAARYGERFSPPKLLRDMAAEGRT 715 Query: 705 FW 706 F+ Sbjct: 716 FY 717 Lambda K H 0.319 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1006 Number of extensions: 45 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 714 Length of database: 724 Length adjustment: 40 Effective length of query: 674 Effective length of database: 684 Effective search space: 461016 Effective search space used: 461016 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory