Alignments for a candidate for fadB in Rhodomicrobium vannielii ATCC 17100

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadB in Rhodomicrobium vannielii ATCC 17100

Align Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate WP_013418529.1 RVAN_RS04240 3-hydroxyacyl-CoA dehydrogenase

Query= SwissProt::P21177
         (729 letters)



>NCBI__GCF_000166055.1:WP_013418529.1
          Length = 672

 Score =  325 bits (833), Expect = 5e-93
 Identities = 230/676 (34%), Positives = 345/676 (51%), Gaps = 55/676 (8%)

Query: 17  IAELVFDAPG-SVNKLDTATVASLGEAIGVLE---QQSDLKGLLLRSNK-AAFIVGADIT 71
           IA  VFD  G S N L     A L   +  +    Q   ++GL++ S K  +FI GA+I 
Sbjct: 20  IAWAVFDVKGQSHNTLGREQFAELDRIVTAVAHGAQDRTVRGLVIMSGKDKSFIAGANIK 79

Query: 72  EFLSLFLVPEEQLSQWLHFANSVFNRLEDLPVPTIAAVNGYALGGGCECVLATDYRLATP 131
           EF  L    EE + + +    +VF+R+E LPVP +AA+NG  LGGG E  LA  YR+AT 
Sbjct: 80  EFDDL--KTEEDVIEAVRAGTAVFDRIEALPVPVVAAINGACLGGGLEFALACHYRIATR 137

Query: 132 D--LRIGLPETKLGIMPGFGGSVRMPRMLGADSALEIIAAGKDVGADQALKIGLVDGVVK 189
           +   RIGLPE KLGI+PG  GS R  R  G  SA+  + AGK +   QA   G VD +V 
Sbjct: 138 EDATRIGLPEVKLGIIPGLNGSARWLRQAGPMSAMPPMLAGKLLRPGQARAAGAVDQLVP 197

Query: 190 AEKLVEGA--KAVLRQAINGDLDWKAKRQPKLEPLKLSKIEATMSFTIAKGMVAQTAGK- 246
               ++ A  KAVL+ A +         QP      L   E   +F +A  M  +TA K 
Sbjct: 198 TRHELKWAARKAVLKNAHS---------QPSRAWQPLLMFEPGRAF-LANRMRKETAAKV 247

Query: 247 ---HYPAPITAVKTIEAAARFGREEALNLENKSFVPLAHTNEARALVGIFLNDQYVKGKA 303
              HYPAP   +   E       ++    E K F PL  ++++R L  +F   + +K +A
Sbjct: 248 RAEHYPAPFRLIDLFEKHGG-SFDDMKRAETKFFAPLMISDQSRNLRRVFRLSEMLKAEA 306

Query: 304 KKLTKDVETPKQAAVLGAGIMGGGIAYQSAWKGVPVVMKDINDKSLTLGMTEAAKLLNKQ 363
            K   D   P +A V+GAG MG  IA     +G+ V ++D + +++   +  A     K+
Sbjct: 307 PK---DGWRPARAHVIGAGTMGADIAMACVREGMEVSLQDNSPEAVEKALKRADAFFKKR 363

Query: 364 LERGKIDGLKLAGVISTIHPTLDYAGFDRVDIVVEAVVENPKVKKAVLAETEQKVRQDTV 423
           + R  ++  K     + +      A   R D+V+EA+ EN   K+AV AE E K++   +
Sbjct: 364 M-RSPLEAEKAK---ARLLADPQGAQIHRADVVIEAIYENLDAKRAVFAEVEPKLKPGAL 419

Query: 424 LASNTSTIPISELANALERPENFCGMHFFNPVHRMPLVEIIRGEKSSDETIAKVVAWASK 483
           LA+NTS++ I ++A+AL+ P    G+HFFNPV  +PLVE+++G  S +E I +  A+ ++
Sbjct: 420 LATNTSSLKIEDIASALQDPSRLIGLHFFNPVTVLPLVEVVKGADSREEEIRRGAAFVTR 479

Query: 484 MGKTPIVVNDCPGFFVNRVLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDV 543
           +GK P++V   PGF VNR L PY  G  Q L +G    KID   E +FG P+GP  L+D+
Sbjct: 480 IGKLPLIVKSSPGFLVNRTLAPYMFGAMQKLAEGTPKEKIDAAAE-EFGMPVGPIELVDI 538

Query: 544 VGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKE 603
           VG+D A     ++    P          +  L    + G+K G GF+++ +  + KP   
Sbjct: 539 VGLDVALSVAKILKLPTPD------DHPLMQLVAEKKLGKKTGEGFYKWVDGKREKPATA 592

Query: 604 EDAAVEDLLAEVSQPKRDFSEEEIIARMMI-PMVNEVVRCLEEGIIATPAEADMALVYGL 662
            + A  DL               ++ R +I P+++E  + L +G++A     D   V+G 
Sbjct: 593 PNFAKADL--------------SVLGRELIAPLIDESEKALADGVVADADHVDAGAVFGA 638

Query: 663 GFPPFHGGAFRWLDTL 678
           GF PF GG   +  +L
Sbjct: 639 GFAPFRGGPLHYRASL 654


Lambda     K      H
   0.318    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1123
Number of extensions: 60
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 729
Length of database: 672
Length adjustment: 39
Effective length of query: 690
Effective length of database: 633
Effective search space:   436770
Effective search space used:   436770
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources