Alignments for a candidate for aacS in Pyrolobus fumarii 1A

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Pyrolobus fumarii 1A

Align acetoacetate-CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_014026520.1 PYRFU_RS04855 acetate--CoA ligase

Query= BRENDA::D6EQU8
         (658 letters)



>NCBI__GCF_000223395.1:WP_014026520.1
          Length = 656

 Score =  235 bits (599), Expect = 5e-66
 Identities = 184/623 (29%), Positives = 286/623 (45%), Gaps = 49/623 (7%)

Query: 61  EWFDVRFSTPYARVLGDRTMPGAQWFPGATLNYAEHALRAAGTRP--DEPALLYVDETHE 118
           EWF       + +VL D   P  +WF G  +N + +A+     R   ++ A+++  E  +
Sbjct: 42  EWFKT-----WDKVLDDSNPPFYRWFVGGLINASYNAVDRHLKRGLRNKAAIIWEGEPGD 96

Query: 119 PAPVTWAELRRQVASLAAELRALGVRPGDRVSGYLPNIPQAVVALLATAAVGGVWTSCAP 178
              +T+ +L R+V   AA L  LG+R GD ++ YLP IP+  + +LA A +G  +T    
Sbjct: 97  TRVLTYYQLYREVNRFAAVLENLGLRKGDTIAFYLPMIPELPIFMLAAARLGITFTVVFS 156

Query: 179 DFGARSVLDRFQQVEPVVLFTVDGYRYGGKEHDRRDTVAELRRELPTLRAV--------- 229
            F A+++ +R    +  VL T DG    GK  + +    E     P++  V         
Sbjct: 157 GFSAKALAERINDAKAKVLVTADGGFRRGKVIELKKIADEALEMAPSVETVIVVKNVTAR 216

Query: 230 ---IHIPLLGTEAPDGTLDWETLTAADAEPVYEQVPFDHPLWVLYSSGTTGLPKAIVQSQ 286
              I +PL  TE  D  L       A  +PV   V   HPL++LY+SGTTG PK +V S 
Sbjct: 217 GAKIDVPL--TEGRDYWLHNLLPAHAKVDPV--PVESTHPLFILYTSGTTGKPKGVVHST 272

Query: 287 GGILVEHLKQLGLHCDLGPGDRFFWYTSTGWMM-WNFLVSG-LLTGTTIVLYDGSPGFPA 344
           GG LV     +    D+ P D  F     GW+   +++V G LL G T V+Y+G+P +PA
Sbjct: 273 GGYLVWVYATMKWVFDVKPDDVMFCTADIGWITGHSYVVFGPLLHGITTVMYEGAPDYPA 332

Query: 345 TDAQWRIAERTGATLFGTSAAYVMACRKAGVHPARDLDLSAIQCVATTGSPLPPDGFRWL 404
            D  W+I E+ G T+F T+   +    + G    +  DLS ++ + T G P+ P+ ++W 
Sbjct: 333 PDRWWQIVEKYGVTIFYTAPTAIRMLMRYGEEWVKKHDLSTLRILGTVGEPINPEAWKWY 392

Query: 405 HDEFAAGG-----ADLWIASVSGGTDVCSCFAGAVPTLPVHIGELQAPGLGTDLQSWDPS 459
           +     GG      D W  + +GG  +    A  +  +P+  G    P  G D       
Sbjct: 393 YT--VVGGERCPIVDTWWQTETGGIMISP--APGIQLVPLKPGSATYPLPGVDADVVCED 448

Query: 460 GDPL-TDEVGELVVTNPMPSMPIRFWNDPDGSRYHDSYFDTY----PGVWRH--GDWITL 512
           G P    + G LV+  P P M +  W DP+  RY  +Y+  +     G W +   D+   
Sbjct: 449 GSPCPPGQRGYLVIKRPWPGMLMTLWGDPE--RYVRTYWQRFSKPEEGKWIYYPADYAMK 506

Query: 513 TSRGSVVIHGRSDSTLNRQGVRMGSADIYEAVERLPEIRESLVIGIEQPDGGYWMPLFVH 572
              G   I GR+D  +   G R+G+A+I  A+   P + E+ V+G   P  G     FV 
Sbjct: 507 DEDGYFWILGRADEVIKVAGHRLGTAEIESALVSHPAVAEAAVVGKPDPVKGEVPVAFVV 566

Query: 573 LAPGATLDDALLDRIKRTIRVNLSPRHVPDEVIEVPGIPHTLTGKRIEVPVKRLLQGTPL 632
           L  G T  + L   +   +R  L P  VP  +  V  +P T +GK +   +K +L G   
Sbjct: 567 LRQGYTPSEELRHELVEHVRKTLGPIAVPAAIFFVEKLPKTRSGKIMRRVIKAVLLGK-- 624

Query: 633 DKAVNPGSIDNLDLLHFYEELAR 655
                PG +  L+     +E+ R
Sbjct: 625 ----TPGDLTTLEDEASLDEIRR 643


Lambda     K      H
   0.320    0.138    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1430
Number of extensions: 93
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 658
Length of database: 656
Length adjustment: 38
Effective length of query: 620
Effective length of database: 618
Effective search space:   383160
Effective search space used:   383160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources