GapMind for Amino acid biosynthesis

 

Alignments for a candidate for leuD in Leptospirillum ferrooxidans C2-3

Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase (uncharacterized)
to candidate WP_014449006.1 LFE_RS04105 3-isopropylmalate dehydratase small subunit

Query= curated2:A1K4A2
         (212 letters)



>NCBI__GCF_000284315.1:WP_014449006.1
          Length = 208

 Score =  239 bits (611), Expect = 2e-68
 Identities = 116/211 (54%), Positives = 151/211 (71%), Gaps = 8/211 (3%)

Query: 1   MKPFTVLDAIVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDVGQPGQDCSN 60
           M+ F  L +IV P+DRANVDTDAIIPKQFLK+I R+G G +LF +WRYL  G        
Sbjct: 1   MEAFKTLKSIVIPIDRANVDTDAIIPKQFLKTIHRTGLGKSLFYDWRYLSDGVT------ 54

Query: 61  RPKNPDFVLNQARYQGAQVLLARDNFGCGSSREHAPWALEDYGFRVIIAPSFADIFFNNS 120
              NPDFV+NQ RY+G ++LL+R+NFG GSSREHAPWAL D+G + IIAPSFADIF+NN 
Sbjct: 55  --PNPDFVINQTRYKGGRILLSRENFGSGSSREHAPWALLDFGIKAIIAPSFADIFYNNC 112

Query: 121 FKNGLLPIKLDAAELDVLFQQCEATEGYRLKVDLAAQTITRPDGKAIAFDVDPFRKECLL 180
           FKNG+LPI++D   +D LF++ + T GY+ +++L +Q I  PDG+ I F++D F K+CLL
Sbjct: 113 FKNGILPIRIDRMIVDGLFKEIDRTPGYQAEINLESQLILLPDGRTIPFEIDAFLKKCLL 172

Query: 181 NGWDDIGLTLRHADKIRDFEAKRRAEHPYYF 211
            G DDI LT      I  +E +RR E P+ F
Sbjct: 173 EGLDDISLTFEKKALITSYEDRRRKEAPWLF 203


Lambda     K      H
   0.323    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 168
Number of extensions: 3
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 212
Length of database: 208
Length adjustment: 21
Effective length of query: 191
Effective length of database: 187
Effective search space:    35717
Effective search space used:    35717
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 45 (21.9 bits)

Align candidate WP_014449006.1 LFE_RS04105 (3-isopropylmalate dehydratase small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))

Software error:

../bin/blast/fastacmd -i /tmp/list.25054.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.25054.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

For help, please send mail to the webmaster (help@microbesonline.org), giving this error message and the time and date of the error.