Align oxepin-CoA hydrolase (EC 3.3.2.12) (characterized)
to candidate WP_017601603.1 D471_RS0127555 phenylacetic acid degradation bifunctional protein PaaZ
Query= BRENDA::P77455 (681 letters) >NCBI__GCF_000341125.1:WP_017601603.1 Length = 677 Score = 643 bits (1658), Expect = 0.0 Identities = 338/680 (49%), Positives = 460/680 (67%), Gaps = 19/680 (2%) Query: 4 LASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAARQFAIEKGAPALRAMTFIE 63 L S+ G+W + + A +GE + +++ G D+AA +A G PALRA+TF + Sbjct: 5 LESYALGSWFTPEDEGAPLADANTGETVARISANGPDVAAMIDYARRVGGPALRALTFHQ 64 Query: 64 RAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEGGIGTLFTYASLGSRELPDDTL 123 RA +LK +A +L K+ FYALS +TGATR D+ VD++GG GTLF+++S G RELP+ T+ Sbjct: 65 RALILKELATYLTGHKDEFYALSHRTGATRRDTMVDVDGGFGTLFSFSSKGRRELPNSTV 124 Query: 124 WPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFPCWGMLEKLAPTWLGGMPAIIKPA 183 + L PL ++G F +H+ TS+ GVAV INAFNFP WGMLEKLAP +L GMP+I+KPA Sbjct: 125 VLDGPLEPLGRKGTFVGQHVYTSRPGVAVQINAFNFPVWGMLEKLAPAFLAGMPSIVKPA 184 Query: 184 TATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDLLDHLDSQDVVTFTGSAATGQMLRVQ 243 T+ LT+A+V+ +V+SGL+PEG++ L+ G+ LLD+L QD+V FTGSAATG +LR Sbjct: 185 GQTSYLTEAVVRRMVESGLLPEGSLQLLVGTHRGLLDNLGPQDIVGFTGSAATGALLREH 244 Query: 244 PNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEFALFIREVVREMTTKAGQKCTAIRRIIV 303 PN+V + +EADSLNC +LG DV + PEF LF+++VV EMT KAGQKCTAIRR+IV Sbjct: 245 PNVVRGGVQLNVEADSLNCSILGPDVRVEDPEFDLFVKQVVTEMTVKAGQKCTAIRRVIV 304 Query: 304 PQALVNAVSDALVARLQKVVVGDPAQEGVKMGALVNAEQRADVQEKVNILLAAGCEIR-- 361 P+ + +AV++A+ RL VVVG +E V+MG L +QRA+V++ V LLA+ + Sbjct: 305 PEPMADAVTEAISDRLSAVVVGAADREDVRMGPLATLDQRAEVRKAVKALLASADVVHGD 364 Query: 362 ------LGGQADLSAAGAFFPPTLLYCPQPDETPAVHATEAFGPVATLMPAQNQRHALQL 415 +G A+ AGAF P LL +PD H EAFGPV+T++ A++L Sbjct: 365 PDEVEVVGADAE---AGAFMSPILLRA-RPD-AGEPHEVEAFGPVSTVITYDGVAQAVEL 419 Query: 416 ACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQILNEESAKESTGHGSPLPQLVHGGP 475 A G GSL G++VT DP +AR+ + A HGRI +L+ + AKESTGHGSPLP LVHGGP Sbjct: 420 AARGTGSLVGSVVTHDPDVAREIVLGLAPWHGRILVLDRDDAKESTGHGSPLPMLVHGGP 479 Query: 476 GRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAAISKQWVRGAKVEEDRIHPFRKYFEELQ 535 GRAGGGEE+GG+R VKH+MQRTA+Q SP M AI+ W G++ + +HPFRK +L+ Sbjct: 480 GRAGGGEEMGGVRGVKHHMQRTAIQASPDMATAITGHWTTGSRRDVGEVHPFRKNLAQLR 539 Query: 536 PGDSLLTPRRTMTEADIVNFACLSGDHFYAHMD-KIAAAESIFGERVVHGYFVLSAAAGL 594 GD++ + RT+T DI +FA +GD FYAH D + AAA +FG V HGY V+S AAGL Sbjct: 540 IGDTISSADRTVTREDIDHFAEFTGDTFYAHTDEEAAAANPLFGGIVAHGYLVVSLAAGL 599 Query: 595 FVDAGVGPVIANYGLESLRFIEPVKPGDTIQVRLTCKRKTLKKQRSAEEKPTGVVEWAVE 654 FVD GPV+AN+G+++LRF+ PVK G+TI+V+LT K+ T + G V W Sbjct: 600 FVDPDPGPVLANFGVDNLRFLTPVKEGETIRVQLTAKQITPRANAD-----YGEVRWDAL 654 Query: 655 VFNQHQTPVALYSILTLVAR 674 V N+ PVA Y +LTLVA+ Sbjct: 655 VTNERDEPVATYDVLTLVAK 674 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1135 Number of extensions: 51 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 681 Length of database: 677 Length adjustment: 39 Effective length of query: 642 Effective length of database: 638 Effective search space: 409596 Effective search space used: 409596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory