Alignments for a candidate for lctP in Hydrogenovibrio halophilus DSM 15072

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Hydrogenovibrio halophilus DSM 15072

Align L-lactate permease (characterized, see rationale)
to candidate WP_019895194.1 A377_RS11960 L-lactate permease

Query= uniprot:Q8EGS2
         (547 letters)



>NCBI__GCF_000384235.1:WP_019895194.1
          Length = 560

 Score =  489 bits (1258), Expect = e-142
 Identities = 268/568 (47%), Positives = 364/568 (64%), Gaps = 43/568 (7%)

Query: 1   MTILQLFASLTPVLSVMIFLVLLRMPASKAMPISMVVTAIAAVFIWQMDTTLLAASVVEG 60
           MT+LQ   +  PVL+V++FLV+LR+PAS+AMP+S+V+ A+ A  +WQM    +AASV+EG
Sbjct: 1   MTLLQFLTAAMPVLAVLVFLVILRLPASRAMPLSLVIVALLAWQVWQMPVDYIAASVLEG 60

Query: 61  LLSAITPLTIIFGAVFLLNTLKYSGAMDTIRAGFTNISADARVQVIIICWLFGAFIEGSA 120
            +     L I+FGA+ LLNTLK SGA+DTIR GF+ IS D RVQ +I+ WLF AF+EG++
Sbjct: 61  WVIGAAILIIVFGAIVLLNTLKVSGAVDTIRQGFSGISPDRRVQTVIVGWLFVAFLEGAS 120

Query: 121 GFGTPAAIGAPLLVLLGVPPVAAAVVALIADSACVSFGAIGLPVLFGMEQGLIQGGVSMA 180
           GFGTPAAI APLL+ LG P +AA  +ALIADSA VS+GA+G PV+ G+ +G+        
Sbjct: 121 GFGTPAAIVAPLLLALGFPALAAVTLALIADSAAVSYGAVGTPVIVGIGEGV-------- 172

Query: 181 AEQFAAHGGTYAGYARYIVMHMITIDLITGTLIPLVMVTMLTGFFGRNKSFKEGLAIWKF 240
                  G TY       V   + ID++  + +PL+MV MLT FFG NKS++EGL  WKF
Sbjct: 173 ------EGITYEQMMEVAVTASL-IDVMVASFLPLLMVVMLTRFFGENKSWREGLGAWKF 225

Query: 241 AIFSGLAFTVPAWIINYLAGPEFPSVIGALIGMAMVIPVARKGYLLPKTPWNDFAENDNQ 300
           A+  G +FT+PA  + +L GPEFP+++G LIG+ + +  AR   L PKT W  FA +  +
Sbjct: 226 ALLGGFSFTLPALGVAWLLGPEFPAILGGLIGLVITVTAARHNILTPKTVWG-FASDTPE 284

Query: 301 DG----------VKLETTAKFSQIA----------------AWTPYIIMAALLVLSR-TV 333
                       VK+E   K   ++                AW PY+++AA+LVL+R  V
Sbjct: 285 TAQTVHKPSPGLVKIEGCEKSPALSCLAVEPVRHACLPLWQAWLPYVLVAAILVLTRLEV 344

Query: 334 APLKAWLSGFNINWTGLLGTELKASFATLYAPGAFFVAVCILGFFLFKMKSPAIKQSIGV 393
            PLK WL    + +  L GT + A    LY PG+ F+ V +L + L ++    +  + G 
Sbjct: 345 LPLKDWLQSVQLTFEQLAGTGISAKLVPLYLPGSVFLLVALLTWALHRVPVRQVTLAWGR 404

Query: 394 SCKSMLPTIISLGASVPMVKIFLNSGANGAGLASMPVALADMLASSMGAVWAWMAPIVGI 453
           S K +LPTII+L ASVPMV+IFLNSG NGA L SMP+ALA + A +    W   AP VG 
Sbjct: 405 SIKVLLPTIIALAASVPMVRIFLNSGENGADLLSMPLALAQLAADTFAGQWPLAAPFVGA 464

Query: 454 FGAFLSGSATFSNMMFSSLQYSVADNIGMNHTLVLALQGIGANAGNMMCVMNVVAAATVV 513
            G+F++GSATFSNMMFS LQ S A+ + M    +LALQ +GANAGNM+CV+NVVAAA+VV
Sbjct: 465 LGSFVAGSATFSNMMFSQLQASTAEALSMPSHWILALQMLGANAGNMICVVNVVAAASVV 524

Query: 514 GMAGRESEIIRKTMPVAIGYALLAGTIA 541
            +AG+E +IIR T+   + YA   G +A
Sbjct: 525 NLAGKEGQIIRYTLVPMLFYATAVGVVA 552


Lambda     K      H
   0.326    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 970
Number of extensions: 49
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 560
Length adjustment: 36
Effective length of query: 511
Effective length of database: 524
Effective search space:   267764
Effective search space used:   267764
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources