Alignments for a candidate for cimA in Methyloferula stellata AR4T

Align (R)-citramalate synthase (EC 2.3.3.21) (characterized)
to candidate WP_020175256.1 A3OQ_RS0110050 citramalate synthase

Query= BRENDA::Q74C76
         (528 letters)



>NCBI__GCF_000385335.1:WP_020175256.1
          Length = 531

 Score =  477 bits (1227), Expect = e-139
 Identities = 266/525 (50%), Positives = 343/525 (65%), Gaps = 14/525 (2%)

Query: 6   LYDTTLRDGTQAEDISFLVEDKIRIAHKLDEIGIHYIEGGWPGSNPKDVAFFKDIKKEKL 65
           L+DTTLRDG Q   + F ++DK  IA  LD++G+ YIEGG+PG+NP D AFF   K+  L
Sbjct: 8   LFDTTLRDGAQTTGVDFSLDDKRHIAALLDDLGLDYIEGGYPGANPTDTAFFA--KRPVL 65

Query: 66  SQAKIAAFGSTRRAKVTPDKDHNLKTLIQAEPDVCTIFGKTWDFHVHEALRISLEENLEL 125
            +A+ AAFG T+RA  +   D  L +L +A+ DV T   KTWD+ V  AL  +L+ENLE 
Sbjct: 66  KKARFAAFGMTKRAGRSVANDPGLASLFEADADVITYVAKTWDYQVRVALGCTLDENLEG 125

Query: 126 IFDSLEYLKANVPEVFYDAEHFFDGYKANPDYAIKTLKAAQDAKADCIVLCDTNGGTMPF 185
           I  S+E   A   EV  D EHFFDGYKANP YA+   KAA  A A  IVLCDTNGG++P 
Sbjct: 126 ISQSVEAAAARGREVILDCEHFFDGYKANPAYALDCAKAAHAAGARWIVLCDTNGGSLPH 185

Query: 186 ELVEIIREVRKHITAP-LGIHTHNDSECAVANSLHAVSEGIVQVQGTINGFGERCGNANL 244
           E+  I+ EV  HI    LGIH HND+E AVANSL AV  G   +QGT+NG GERCGNANL
Sbjct: 186 EVERIVTEVTAHIPGSHLGIHAHNDTEHAVANSLAAVRAGARHIQGTLNGLGERCGNANL 245

Query: 245 CSIIPALKLKMKREC-----IGDDQLRKLRDLSRFVYELANLSPNKHQAYVGNSAFAHKG 299
            SIIP L LK          +  ++L ++  +S  + EL N  PN+H  YVG SAFA K 
Sbjct: 246 MSIIPTLLLKKDYADHYEIGVPLEKLAQITKISHALDELLNRQPNRHAPYVGASAFATKA 305

Query: 300 GVHVSAIQRHPETYEHLRPELVGNMTRVLVSDLSGRSNILAKAEEFNIKMDSKDPVTLEI 359
           G+H SA+ + P TYEH+ PE VGN  +V+VSD +GRSNILA+ E   + +   DP    +
Sbjct: 306 GIHASAVLKDPATYEHVTPESVGNQRKVIVSDQAGRSNILAELERIGVMLAKDDPRINRL 365

Query: 360 LENIKEMENRGYQFEGAEASFELLMKRALGTHRKFFSVIGFRVIDEKRHEDQKPL---SE 416
           L+ +KE E+ GY +EGAEASFELL++R LG    +F V  F V  E+RH  Q  L   SE
Sbjct: 366 LDEVKEKESLGYAYEGAEASFELLVRRVLGEVPDYFDVERFSVGVERRHNAQGDLVTVSE 425

Query: 417 ATIMVKVGGKIEHTAAEGNGPVNALDNALRKALEKFYPRLKEVKLLDYKVRVLPAGQGTA 476
           A + V+V  ++  +AAEG GPVNALD ALRK L ++   +++V+L+DY+VRV     GT 
Sbjct: 426 AVVKVRVHDELLLSAAEGIGPVNALDLALRKDLGRYQRFIEDVELVDYRVRVFQG--GTD 483

Query: 477 SSIRVLIESGDKES-RWGTVGVSENIVDASYQALLDSVEYKLHKS 520
           +  RVL+E  DK+  RW TVGVS NI+DAS+QAL D++ YKL K+
Sbjct: 484 AVTRVLVEFADKDDMRWSTVGVSANIIDASFQALTDAITYKLLKA 528


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 723
Number of extensions: 29
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 528
Length of database: 531
Length adjustment: 35
Effective length of query: 493
Effective length of database: 496
Effective search space:   244528
Effective search space used:   244528
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_020175256.1 A3OQ_RS0110050 (citramalate synthase)
to HMM TIGR00977 (cimA: citramalate synthase (EC 2.3.1.182))

Software error:

../bin/blast/fastacmd -i /tmp/list.18779.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.18779.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

For help, please send mail to the webmaster (help@microbesonline.org), giving this error message and the time and date of the error.