Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_022948469.1 H035_RS0107990 acetate--CoA ligase
Query= SwissProt::P27550 (652 letters) >NCBI__GCF_000421465.1:WP_022948469.1 Length = 650 Score = 847 bits (2187), Expect = 0.0 Identities = 401/647 (61%), Positives = 492/647 (76%), Gaps = 7/647 (1%) Query: 1 MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQG-KILDWIKPYQKVKNTS 59 MSQ + +P I R ++ +Y +Y +S++ PD FW EQ + + W +P++ V + Sbjct: 1 MSQAKIYPVPPEIETRAHMSADRYRRLYARSLSDPDGFWAEQAERFVSWDQPWKTVSDWD 60 Query: 60 FAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDASQSKHISYKELHRDVCR 119 F V I+W+E G LN NCLDRHL+ GD TAIIWE DD + S+ +SY+ELH +VC+ Sbjct: 61 F--NRVHIRWFEGGRLNACFNCLDRHLESRGDETAIIWESDDPAHSRSLSYRELHEEVCK 118 Query: 120 FANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSN 179 FAN L + G+ KGD V IY+PM+PEAAVAMLACARIGA+HSV+FGGFS +A+ RI D+ Sbjct: 119 FANVLKQHGVAKGDRVCIYLPMIPEAAVAMLACARIGAIHSVVFGGFSADALRDRIRDAE 178 Query: 180 SRLVITSDEGVRAGRSIPLKKNVDDALKN-PNVTSVEHVVVLKRTGGKIDWQEGRDLWWH 238 + +IT+D G+R G+ + LK N D AL P VT HV+V++R+G + WQEGRD+W+H Sbjct: 179 AHTLITADAGLRGGKHVSLKANADQALSQCPGVT---HVIVVRRSGTDVSWQEGRDIWYH 235 Query: 239 DLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPG 298 + + A+ Q + E M+AEDPLFILYTSGSTGKPKGVLHTTGGYLVYA +T YVFDYH G Sbjct: 236 EAMASAAPQCEPEIMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYATMTHHYVFDYHEG 295 Query: 299 DIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAP 358 ++YWCTADVGW+TGH+YL+YGPL GA T+MFEGVP WP P R QVVDKH+VNI YTAP Sbjct: 296 EVYWCTADVGWITGHTYLVYGPLCNGAVTVMFEGVPMWPEPDRFWQVVDKHRVNIFYTAP 355 Query: 359 TAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTET 418 TAIRALM GD+ + T R SLR+LGSVGEPINPEAWEWY + +G +CPVVDTWWQTET Sbjct: 356 TAIRALMRVGDEYVGRTSRRSLRLLGSVGEPINPEAWEWYHRVVGESRCPVVDTWWQTET 415 Query: 419 GGFMITPLPGATELKAGSATRPFFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLF 478 GG +ITPLPGAT LK GSAT PFFG++P ++D++ N L+G+ EG L + SWPGQARTL+ Sbjct: 416 GGILITPLPGATPLKPGSATLPFFGIEPVILDDKNNELQGSAEGILALKRSWPGQARTLY 475 Query: 479 GDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVA 538 +HERF +TYF F YF+GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVA Sbjct: 476 KNHERFVETYFKPFPGHYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVA 535 Query: 539 HPKIAEAAVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHW 598 HP +AEAAVVG PH+IKGQ IYAYV G E PEL + + V +EIGP+A PD + + Sbjct: 536 HPDVAEAAVVGYPHDIKGQGIYAYVIPKRGVETGPELAQSLVDLVNREIGPIAKPDAIQF 595 Query: 599 TDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTLADPGVVEKLLEEK 645 LPKTRSGKIMRRILRKIA+ LGDTSTLADP V++ LLE + Sbjct: 596 APDLPKTRSGKIMRRILRKIASQQIDQLGDTSTLADPSVIDHLLENR 642 Lambda K H 0.317 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1435 Number of extensions: 55 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 652 Length of database: 650 Length adjustment: 38 Effective length of query: 614 Effective length of database: 612 Effective search space: 375768 Effective search space used: 375768 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory