Align Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate WP_022949690.1 H035_RS19930 ABC transporter ATP-binding protein
Query= TCDB::Q72L52 (376 letters) >NCBI__GCF_000421465.1:WP_022949690.1 Length = 289 Score = 155 bits (392), Expect = 1e-42 Identities = 91/250 (36%), Positives = 143/250 (57%), Gaps = 11/250 (4%) Query: 4 VRLEHVWKRF----GKVVAVKDFNLETEDGEFVVFVGPSGCGKTTTLRMIAGLEEISEGN 59 + ++HV F G + + NL+ + GEFV +G SGCGK+T L ++AGL + +EG Sbjct: 5 LNIDHVSITFPTEKGPLNVLNRINLKMKQGEFVSLIGHSGCGKSTVLNIVAGLLQPTEGG 64 Query: 60 IYIGDRLVNDVPPKDRDIAMVFQNYALYPHMNVYENMAFGL-RLRRYPKDEIDRR--VKE 116 + + ++ VND P+ A+VFQN++L P ++VY+N+ + R+ R K + +R Sbjct: 65 VVLENKEVNDPGPER---AVVFQNHSLLPWLSVYDNVRLAVDRVFRKTKSKAERHEWTLT 121 Query: 117 AARILKIEHLLNRKPRELSGGQRQRVAMGRAIVREPKVFLMDEPLSNLDAKLRVEMRAEI 176 ++ + H L RKP E+SGG +QRV + RA+ EPKV LMDEP LD+ R M+ + Sbjct: 122 NLELVHMSHALERKPHEISGGMKQRVGIARALAMEPKVLLMDEPFGALDSLTRTHMQDSL 181 Query: 177 AKLQRRLGVTTIYVTHDQVEAMTLGHRIVVMKDGEIQQVDTPLNLYDFPANRFVAGFIGS 236 +Q RLG T I +THD EA+ L R+V+M +G ++ L++ D P R + Sbjct: 182 MDIQARLGNTVIMITHDVDEAVLLSDRVVMMTNGPAARIGEILSI-DLPRPRDRLALAEN 240 Query: 237 PSMNFVRAGV 246 + N RA + Sbjct: 241 STFNHYRAEI 250 Lambda K H 0.320 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 274 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 289 Length adjustment: 28 Effective length of query: 348 Effective length of database: 261 Effective search space: 90828 Effective search space used: 90828 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory