Alignments for a candidate for glpD in Methylohalobius crimeensis 10Ki

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpD in Methylohalobius crimeensis 10Ki

Align glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) (characterized)
to candidate WP_022949984.1 H035_RS20290 glycerol-3-phosphate dehydrogenase/oxidase

Query= BRENDA::P43304
         (727 letters)



>NCBI__GCF_000421465.1:WP_022949984.1
          Length = 527

 Score =  379 bits (972), Expect = e-109
 Identities = 226/562 (40%), Positives = 321/562 (57%), Gaps = 39/562 (6%)

Query: 56  SREAQLLTLQN-TSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKL 114
           +R A L  L++ T+ +D++VIGGGATG G A+DA +RG +TAL+E+ DF+ GTSSRSTKL
Sbjct: 2   NRSATLERLRSETAPWDVVVIGGGATGLGVAVDAASRGYRTALLEQHDFAKGTSSRSTKL 61

Query: 115 IHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYW 174
           IHGGVRYL++  ++L       V+E+LHERA LL  APHL  PL  ++P Y+WWQ  YY 
Sbjct: 62  IHGGVRYLRQGDLQL-------VRESLHERAVLLANAPHLVTPLGFVMPAYRWWQQAYYA 114

Query: 175 VGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIAL 234
            G+KLYDL+AG   +  S  LS+S  L++ P LQ   L G ++Y+D Q +DAR+ + +A 
Sbjct: 115 AGLKLYDLLAGRRNIAPSGRLSRSETLDYLPTLQSSHLRGGVLYFDAQFDDARLAVTLAR 174

Query: 235 TAARYGAATANYMEVVSLLKKTDPQTGKVRVSGARCKDVLTGQEFDVRAKCVINATGPFT 294
           TAA  GAA ANY  V  L K         R+ G    D  TG+ F + AK V+NATG F+
Sbjct: 175 TAADRGAAVANYTRVTGLRKANG------RIKGVDAVDEETGETFAIPAKTVVNATGVFS 228

Query: 295 DSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGT 354
           D+VR++DD  A  +  PS GVH+V+P  + P    LL P T DGRV+F +PWQ   + GT
Sbjct: 229 DTVRRLDDPRARPMIAPSRGVHLVLPKRFLPGENALLIPRTDDGRVLFAIPWQDRVVVGT 288

Query: 355 TDTPTD-VTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIRPLVTDPKSADT 413
           TD P    +  P  S ++  ++L   R YL+        DVL+ ++G+RPLV    +  T
Sbjct: 289 TDVPVKRPSLEPPSSRQEWEYLLTHARRYLT--NPPTESDVLSTFAGLRPLVKADGNTST 346

Query: 414 QSISRNHVVDISESGLITIAGGKWTTYRSMAEDTINAAVKTHNLKAGPSRTVGLFLQGGK 473
            ++SR+H +  S SGL+TIAGGKWTTYR MA DT++  ++   L      T  L L G  
Sbjct: 347 AALSRDHTLLASPSGLVTIAGGKWTTYRKMARDTVDRLIELGPLPPAHCVTEKLRLHGWT 406

Query: 474 DWSPTLYIRLVQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIVGVRLVSEFPYI 533
           D       R  ++YG +++  + L  T                   P     L    PY 
Sbjct: 407 DRPLPEGHR--REYGADADAIRQLIET-------------------PDKETLLHPHLPYR 445

Query: 534 EAEVKYGIK-EYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQL 592
            AEV++ ++ E A T  D+++RRTR   L+ +A+ EA P +  +M  EL+ D    Q Q+
Sbjct: 446 LAEVRWAVRLEMARTVEDVLARRTRALLLDARASAEAAPMVARVMAEELSRDAAWIQAQI 505

Query: 593 ETARKFLYYEMGYKSRSEQLTD 614
           +  ++     +   +RS   TD
Sbjct: 506 QVFQRLAKSYLIVPNRSRPKTD 527


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 772
Number of extensions: 19
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 727
Length of database: 527
Length adjustment: 37
Effective length of query: 690
Effective length of database: 490
Effective search space:   338100
Effective search space used:   338100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources