Alignments for a candidate for ech in Methylohalobius crimeensis 10Ki

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Methylohalobius crimeensis 10Ki

Align 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized)
to candidate WP_022950068.1 H035_RS0116520 crotonase

Query= BRENDA::A4YDS4
         (651 letters)



>NCBI__GCF_000421465.1:WP_022950068.1
          Length = 677

 Score =  187 bits (475), Expect = 1e-51
 Identities = 135/371 (36%), Positives = 195/371 (52%), Gaps = 14/371 (3%)

Query: 3   VTVIGSGVMGHGIAELAAIAGNEVWMNDISTEILQQAMERIKWSLSKLRESGSLKEGVEQ 62
           V V+G+G MG  IA      G    + D     +  A++R  + L K R     +E   +
Sbjct: 306 VHVVGAGTMGGDIAAWCVSRGLTAGLQDREPRYIAPAVKRA-YRLFKRRFKAKHEETAAR 364

Query: 63  VLARIHPETDQAQALKGSDFVIEAVKEDLELKRTIFRNAEAHASPSAVLATNTSSLPISE 122
              R+ P++   Q  + +D VIEA+ E+L  KR ++R  E+  +P A+LATNTSSLP+ E
Sbjct: 365 --DRLIPDSKGFQVDR-ADIVIEAIVENLNAKRALYREIESKLAPDALLATNTSSLPLEE 421

Query: 123 IASVLKSPQRVVGMHFFNPPVLMPLVEIVRGKDTSDEVVKTTAEMAKSMNKETIVVKDVP 182
           +A  L+ P+R+VGMHFFNP   M LVE+VRG  T + V    A   +++++  + VK  P
Sbjct: 422 LAEGLQHPERLVGMHFFNPVAKMQLVEVVRGSHTDEGVADRAAAFVRAIDRLPLPVKSAP 481

Query: 183 GFFVNRVLLRIMEAGCYLVEKGIASIQEVDSSAIEELGFPMGVFLLADYTGLDIGYSVWK 242
           GF VNRVL+  +     L+E+GI +   VD +A  E G PMG   LAD  GLDI  +V +
Sbjct: 482 GFLVNRVLMPYLLEAVVLLEEGIPA-DAVDRAA-TEFGMPMGPIRLADTVGLDICLAVAE 539

Query: 243 AVTARGFKAFPCSSTEKLVSQGKLGVKSGSGYYQYP--SPGKFVRPTLPSTSKKLGRYLI 300
            +T       P    +K V+ G+LGVKSG+G+Y+Y    P  + +   P   + +   LI
Sbjct: 540 ILTENLGGEVPRLLRDK-VAAGELGVKSGTGFYRYKRGKPIVWRKSASPLPQETIEERLI 598

Query: 301 SPAVNEVSYLLREGIVGKDD-AEKGCVLGLGLPK---GILSYADEIGIDVVVNTLEEMRQ 356
              +NE   +LRE +V   D  + G V G G      G + Y  + GI      LEE   
Sbjct: 599 LRLLNECVAVLREQVVEDADLVDAGMVFGAGFAPFRGGPMHYLRQRGIAETQQKLEEFAS 658

Query: 357 TSGMDHYSPDP 367
           T G   + PDP
Sbjct: 659 TYG-PRFQPDP 668



 Score = 93.2 bits (230), Expect = 3e-23
 Identities = 63/176 (35%), Positives = 99/176 (56%), Gaps = 6/176 (3%)

Query: 407 LAWIVLNRP-TRYNAINGDMIREINQALDSLEEREDVRVIAITGQGRVFSAGADVTEFGS 465
           +AW+  +   +  N ++ D++ E+   LD   +     ++ ++ +   F AGADV EF  
Sbjct: 16  IAWLHFDHAESNANVLSADVLAELGSVLDRFHKNPPRGLVILSDKPSGFIAGADVKEFIQ 75

Query: 466 LTPV-KAMIASRKFHEVFMKIQFLTKPVIAVINGLALGGGMELALSADFRVASKTAE--M 522
           LT   +A+   +  H+    +  L  P +A I+G  LGGG+ELAL+  +RVA   A+  +
Sbjct: 76  LTERHRALSLIQVAHKRLQSLATLPCPTVARIHGYCLGGGLELALACRYRVALDDAKTRL 135

Query: 523 GQPEINLGLIPGGGGTQRLSRLSGRKG-LELVLTGRRVKAEEAYRLGIVEFLAEPE 577
           G PE+ LG+ PG GG+ RL R  G     +L+L+GR V A +A RLG+V+  A PE
Sbjct: 136 GLPEVLLGIHPGFGGSVRLIRRIGAPAAFQLMLSGRTVDAAKAARLGLVDH-AVPE 190



 Score = 48.5 bits (114), Expect = 1e-09
 Identities = 45/160 (28%), Positives = 67/160 (41%), Gaps = 19/160 (11%)

Query: 299 LISPAVNEVSYLLREGIVGKDDAEKGCVLGLGLPKGILSYADEIGIDVVVNTLEEMRQTS 358
           ++ P + E   LL EGI    DA        G+P G +  AD +G+D+ +   E + +  
Sbjct: 488 VLMPYLLEAVVLLEEGIPA--DAVDRAATEFGMPMGPIRLADTVGLDICLAVAEILTENL 545

Query: 359 GMDHYSPDPLLLSMVKEGKLGRKSGQGFHTYAHEEAKYSTIVVRVEPPLAWIVLNRPTRY 418
           G +      LL   V  G+LG KSG GF+ Y   +            P+ W     P   
Sbjct: 546 GGE---VPRLLRDKVAAGELGVKSGTGFYRYKRGK------------PIVWRKSASPLPQ 590

Query: 419 NAINGDMI-REINQALDSLEEREDVRVIAITGQGRVFSAG 457
             I   +I R +N+ +  L E + V    +   G VF AG
Sbjct: 591 ETIEERLILRLLNECVAVLRE-QVVEDADLVDAGMVFGAG 629


Lambda     K      H
   0.316    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 878
Number of extensions: 41
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 651
Length of database: 677
Length adjustment: 38
Effective length of query: 613
Effective length of database: 639
Effective search space:   391707
Effective search space used:   391707
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources