Align L-lactate dehydrogenase (EC 1.1.1.27) (characterized)
to candidate WP_026608128.1 METAC_RS0118225 malate dehydrogenase
Query= BRENDA::Q8I8U5 (330 letters) >NCBI__GCF_000427445.1:WP_026608128.1 Length = 321 Score = 327 bits (837), Expect = 3e-94 Identities = 163/319 (51%), Positives = 224/319 (70%), Gaps = 5/319 (1%) Query: 9 RPKIAMVGSGMIGGTMAFLCSLRELGDVVLFDVVPNMPMGKAMDISHNSSVVDTGITVYG 68 R KIA++G+G IGGT+A L +L+ELGD+VLFD+ P GKA+D++ + + ++ G Sbjct: 3 RSKIALIGAGQIGGTLAHLAALKELGDIVLFDIAEGTPQGKALDLAESGPIHGFNASLSG 62 Query: 69 SNSYECLKGADVVIITAGITKIPGKSDKEWSRMDLLPVNIKIMREVGAAIKSYCPNAFVI 128 +NSY + GADVVI+TAG+ + PG S R DLL +N+K+M VG I+ + P AFVI Sbjct: 63 ANSYSAIAGADVVIVTAGVPRKPGMS-----RDDLLGINLKVMEAVGDGIRQFAPQAFVI 117 Query: 129 NITNPLDVMVAALQESSGLPHHRICGMAGMLDSSRFRRMIADKLEVSPRDVQGMVIGVHG 188 ITNPLD MV ALQ+++ LP RI GMAG+LDS+RFR +A++L+VS DV V+G HG Sbjct: 118 CITNPLDAMVWALQKAARLPAQRIVGMAGVLDSARFRYFLAEELDVSVEDVTAFVLGGHG 177 Query: 189 DHMVPLSRYATVNGIPLSEFVKKGWIKQEEVDDIVQKTKVAGGEIVRLLGQGSAYYAPGA 248 D MVPL RY+TV GIPL + V GW Q +D IV++T+ GGEIV L GSA+YAP A Sbjct: 178 DDMVPLLRYSTVAGIPLPDLVAMGWTTQARLDAIVERTRKGGGEIVNHLKTGSAFYAPAA 237 Query: 249 SAIQMAESYLKDRKRVMVCSCYLQGQYGVQNHYLGVPCVIGGRGVEKIIELELTAQERQE 308 SAI MA++YLKD++RV+ C+ L G+YGV N Y+GVP +IG GVEKI+E++L ERQ Sbjct: 238 SAIAMADAYLKDKRRVIPCAAQLNGEYGVDNLYVGVPVIIGADGVEKIVEVKLDPTERQM 297 Query: 309 LQGSIDEVKEMQKAIAALD 327 S++ V+ + +A ++ Sbjct: 298 FDKSVESVRNLVEACKTIN 316 Lambda K H 0.319 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 330 Number of extensions: 13 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 330 Length of database: 321 Length adjustment: 28 Effective length of query: 302 Effective length of database: 293 Effective search space: 88486 Effective search space used: 88486 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory