Align high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF (characterized)
to candidate WP_027180506.1 G496_RS0118045 ABC transporter ATP-binding protein
Query= CharProtDB::CH_003736 (237 letters) >NCBI__GCF_000429985.1:WP_027180506.1 Length = 235 Score = 246 bits (627), Expect = 4e-70 Identities = 131/234 (55%), Positives = 166/234 (70%), Gaps = 2/234 (0%) Query: 5 MLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGKTTLLGTLCGDPRATSGRIVF 64 ML ++ +YG IQAL V + IN GEI+TLIGANGAGKTT L ++ G SG I+F Sbjct: 1 MLELKGINTYYGNIQALRNVDITINVGEIITLIGANGAGKTTTLMSVSGVVPPRSGEILF 60 Query: 65 DDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLAMGGFFAERDQ-FQERIKWVYEL 123 + K + KI++ + VPEGR +F +T+ ENL MG F E ++ I+ V+ L Sbjct: 61 EGKKLNGCSPEKIVKMGICQVPEGRLIFPELTIMENLDMGAFLREDPAGIKDDIEMVFSL 120 Query: 124 FPRLHERRIQRAGTMSGGEQQMLAIGRALMSNPRLLLLDEPSLGLAPIIIQQIFDTIEQL 183 FP L ERR Q GT+SGGEQQMLAI RALM+ PRLLLLDEPSLGLAP+II+QIF+ I+++ Sbjct: 121 FPILKERRRQEGGTLSGGEQQMLAISRALMARPRLLLLDEPSLGLAPLIIKQIFEIIKKI 180 Query: 184 REQ-GMTIFLVEQNANQALKLADRGYVLENGHVVLSDTGDALLANEAVRSAYLG 236 E+ G T+FLVEQNAN ALK A RGYV+ENG + LSDT + LL+NE V+ AYLG Sbjct: 181 NEENGTTVFLVEQNANLALKTAHRGYVMENGEITLSDTSENLLSNEDVKKAYLG 234 Lambda K H 0.321 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 180 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 237 Length of database: 235 Length adjustment: 23 Effective length of query: 214 Effective length of database: 212 Effective search space: 45368 Effective search space used: 45368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory