Alignments for a candidate for paaE in Dechloromonas agitata is5

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaE in Dechloromonas agitata is5

Align 1,2-phenylacetyl-CoA epoxidase, subunit E; 1,2-phenylacetyl-CoA epoxidase, reductase subunit; 1,2-phenylacetyl-CoA monooxygenase, subunit E; EC 1.-.-.- (characterized)
to candidate WP_027456464.1 K420_RS0101335 phenylacetate-CoA oxygenase/reductase subunit PaaK

Query= SwissProt::P76081
         (356 letters)



>NCBI__GCF_000519045.1:WP_027456464.1
          Length = 365

 Score =  305 bits (782), Expect = 9e-88
 Identities = 162/364 (44%), Positives = 234/364 (64%), Gaps = 18/364 (4%)

Query: 4   FHSLTVAKVESETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSIC--- 60
           FH LT+ +V  E   +V ITF +P   +E + F+PGQ LTL+A  +G+++RR YSI    
Sbjct: 6   FHELTIKRVSPEAAGSVAITFDIPAAARETFDFQPGQFLTLRARVEGQDVRRNYSISSPK 65

Query: 61  -RSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGHFGYQ-PQAERQGRYLAI 118
            R    GE+ + ++ +EGG FS +A + ++ G  L+VM P+G F  + P+A  +   +  
Sbjct: 66  SRLAKAGELEIGIRPVEGGLFSNWAAQTLKAGAKLDVMPPEGRFTIKKPRALHR---VGF 122

Query: 119 AAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQALADLKDKYPQRLQLLCIF 178
           AAGSGITP+L+I ATTL+ +PES+FTL+YGNR   S+MF +AL DLKD+YP RL L+ I 
Sbjct: 123 AAGSGITPILSIAATTLEEQPESKFTLVYGNRRMSSVMFNEALQDLKDRYPDRLTLIHIL 182

Query: 179 SQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKT 238
           S++  + DLL GRIDG K+Q++  +L+     DE FICGP AM++  E AL A G+P+  
Sbjct: 183 SRQAQEVDLLQGRIDGAKVQAIIDTLLPVASMDEVFICGPEAMIEATERALLAAGVPENR 242

Query: 239 IHLERFNT-PGTRVKRSVNVQSDGQK--------VTVRQDGRDREIVLNADDESILDAAL 289
           +H ERF + P    K   +  +   K        +T+  DG++ E+ + A DE +LDAAL
Sbjct: 243 VHTERFTSGPAQAAKIQADTDAAPHKQAAAKDIALTIVLDGKEHELHIGA-DEHVLDAAL 301

Query: 290 RQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYVLSCQALPLTSDVVVD 349
             G DLP++CK GVC TC+ KVL G+V M+ N++LE DE+  G+VLSCQA   T ++ V 
Sbjct: 302 DAGLDLPFSCKAGVCCTCRAKVLCGEVVMDKNFTLEADEVKQGFVLSCQARATTKNLTVS 361

Query: 350 FDAK 353
           FD +
Sbjct: 362 FDER 365


Lambda     K      H
   0.320    0.135    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 346
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 365
Length adjustment: 29
Effective length of query: 327
Effective length of database: 336
Effective search space:   109872
Effective search space used:   109872
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources