Align methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_027457187.1 K420_RS0105510 methylmalonyl-CoA mutase
Query= BRENDA::P11653 (728 letters) >NCBI__GCF_000519045.1:WP_027457187.1 Length = 716 Score = 862 bits (2228), Expect = 0.0 Identities = 436/704 (61%), Positives = 543/704 (77%), Gaps = 10/704 (1%) Query: 25 FEELAAKAGTGE-----AWETAEQIPVGTLFNEDVYKDMDWLDTYAGIPPFVHGPYATMY 79 +E++AAK G W T E + V L+ + +++ + DT G+ P++ GP TMY Sbjct: 14 WEKVAAKQSPGGDVNNLVWNTPEGLAVKALYTKKDVENLAFADTLPGVAPYLRGPQPTMY 73 Query: 80 AFRPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMA 139 A +PWTIRQYAGFSTA+ SNAFYR+ LAAG +G+SVAFDL THRGYDSDNPRV GDVG A Sbjct: 74 AVKPWTIRQYAGFSTAEASNAFYRKALAAGGQGVSVAFDLATHRGYDSDNPRVVGDVGKA 133 Query: 140 GVAIDSIYDMRELFAGIPLDQMSVSMTMNGAVLPILALYVVTAEEQGVKPEQLAGTIQND 199 GVAIDS+ DM+ LF GIPLD++SVSMTMNGAVLPILA Y+V AEEQGVK EQL+GTIQND Sbjct: 134 GVAIDSVEDMKILFDGIPLDKISVSMTMNGAVLPILAGYIVAAEEQGVKQEQLSGTIQND 193 Query: 200 ILKEFMVRNTYIYPPQPSMRIISEIFAYTSANMPKWNSISISGYHMQEAGATADIEMAYT 259 ILKEFMVRNTYIYPP+PSM+II++IF+YT+ NMPK+NSISISGYH+QEAGA IE+A+T Sbjct: 194 ILKEFMVRNTYIYPPKPSMKIIADIFSYTAQNMPKFNSISISGYHIQEAGANQAIELAFT 253 Query: 260 LADGVDYIRAGESVGLNVDQFAPRLSFFWGIGMNFFMEVAKLRAARMLWAKLVHQFGPKN 319 LADG++Y+R G + G++VD FA RLSFFW +GMNF++E+AK+RAAR+LW +++ FG KN Sbjct: 254 LADGMEYVRTGIASGMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWHRIMSGFGAKN 313 Query: 320 PKSMSLRTHSQTSGWSLTAQDVYNNVVRTCIEAMAATQGHTQSLHTNSLDEAIALPTDFS 379 KSM LRTHSQTSGWSLT QD YNNVVRT IEAMAA G TQSLHTN+LDEAIALPT+FS Sbjct: 314 AKSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFS 373 Query: 380 ARIARNTQLFLQQESGTTRVIDPWSGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIEK 439 ARIARNTQL +Q+E+ T VIDPW+GS +E+LT D+A KAWG IQE+E +GGM KA+E Sbjct: 374 ARIARNTQLIIQEETHITNVIDPWAGSYMMEKLTQDMADKAWGIIQEIEAMGGMTKAVES 433 Query: 440 GIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLEHEPPLDVLKVDNSTVLAEQKAKLVKL 499 G KM++E AA QARIDSG+ ++GVNKY+L E P+++L +DN V Q A+L + Sbjct: 434 GWAKMQVETCAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDIDNHAVREAQIARLKNI 493 Query: 500 RAERDPEKVKAALDKITWAAGNPDDKDPDRNLLKLCIDAGRAMATVGEMSDALEKVFGRY 559 RA RD V AALD +T AA + + NLL L + A R ATVGE+SDALEK+FGR+ Sbjct: 494 RATRDTAAVNAALDALTKAA-----ETGEGNLLDLTVKAIRLRATVGEVSDALEKIFGRF 548 Query: 560 TAQIRTISGVYSKEVKNTPEVEEARELVEEFEQAEGRRPRILLAKMGQDGHDRGQKVIAT 619 A +TISGVY V+ EE + V +F + EGRRPRI++AK+GQDGHDRG KV+AT Sbjct: 549 RANNQTISGVYGGVVEGQESWEEIKAAVAKFAEEEGRRPRIMIAKLGQDGHDRGAKVVAT 608 Query: 620 AYADLGFDVDVGPLFQTPEETARQAVEADVHVVGVSSLAGGHLTLVPALRKELDKLGRPD 679 AYADLGFD+D+GPLFQTPEE ARQA+E DVH +G SSLA GH TLVP + + L + G D Sbjct: 609 AYADLGFDIDMGPLFQTPEEAARQAIENDVHAIGCSSLAAGHKTLVPQIIQCLKEQGADD 668 Query: 680 ILITVGGVIPEQDFDELRKDGAVEIYTPGTVIPESAISLVKKLR 723 I++ GGVIP QD+D L GA I+ PGT I +SA ++++++ Sbjct: 669 IIVFAGGVIPAQDYDALYAAGAKAIFGPGTRIEDSARKVLEEIK 712 Lambda K H 0.317 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1349 Number of extensions: 42 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 728 Length of database: 716 Length adjustment: 40 Effective length of query: 688 Effective length of database: 676 Effective search space: 465088 Effective search space used: 465088 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory