Align indolepyruvate ferredoxin oxidoreductase (subunit 2/2) (EC 1.2.7.8) (characterized)
to candidate WP_028314756.1 G491_RS0111840 indolepyruvate ferredoxin oxidoreductase
Query= BRENDA::O07835 (647 letters) >NCBI__GCF_000429905.1:WP_028314756.1 Length = 644 Score = 404 bits (1038), Expect = e-117 Identities = 254/649 (39%), Positives = 355/649 (54%), Gaps = 40/649 (6%) Query: 12 KPGERVLLLGNHAIARGALEANIAVFAAYPGTPSSELTDTMAAVAKKAGVYMEYSTNEKV 71 K GE+VL +GN AIARGALEA ++V A YPGTPSSE+ + +A V+K+A +Y+E+S NEK+ Sbjct: 8 KKGEKVLFMGNQAIARGALEAGVSVAAGYPGTPSSEIIENLADVSKEANMYVEWSANEKI 67 Query: 72 AFETALAAAWSGLRAMTAMKHVGLNVAADSFLSSVGMGVEGGFVIMVADDPSMWSSQNEQ 131 A E A AA+++GLR+M MK G+NVA+D L V G GG V++ DDP SS NE Sbjct: 68 AVEVAAAASFAGLRSMCTMKQNGVNVASDFLLHLVLSGTRGGMVLVPCDDPGALSSINEG 127 Query: 132 DTRVYAKFANVPVLEPSSPHEAKEMTKYAFELSEKFKHFVILRTTTRSSHARGDVVLGEL 191 + R +AK +P+LE EAK+M K+AFELSE+ + V+ R+ TR SHA G V LGE+ Sbjct: 128 EARHFAKLLEIPLLEAGDFQEAKDMLKWAFELSEEIHNLVMFRSVTRLSHASGSVELGEI 187 Query: 192 PEEIKTGKRKFGKFKKDPTRFVDVPAHARKFHPLILEKIEKIREELNNCPFNWIEGKEDA 251 P K F DP V A H L +K++K E PFN G E+ Sbjct: 188 PHTEPQAKFVNNGFILDPLEGPIVSAPVGYKHGLQQDKLKKAEELFEESPFNTYVGPENP 247 Query: 252 KVGIIAPGLSYAYVKEALAWLGVED-VKILKLGTPFPVPYGLLGKFFDGLEKVLIVEELE 310 ++ +I + Y +EA+ +D V +LKLGT FP+P L+ K +KVL+VEE+ Sbjct: 248 EILLITSSGATLYSREAIHLCNAKDKVGLLKLGTTFPLPSKLIAKHLKTTDKVLVVEEVL 307 Query: 311 PVVEEQVKTWAYDKGLRI---PIHGK--DLVPRVYEMTTRRAVEAIAKFLGLETPINFAE 365 P +EE +K A + + +GK +P E+ A+A LG ++ Sbjct: 308 PFMEENLKVLAQELAPEVGQKTFYGKRSGNIPMTGELNPDLVATALATLLG----DAYSP 363 Query: 366 IDEKYE-KVSQIV----PPRPPSLCPACPHRNSFFAIRK---AAGPKAIYPSDIGCYTLG 417 D++Y+ K +Q V P R + CP CPHR SF++I A + DIGCY++ Sbjct: 364 QDQEYQTKAAQQVFFNAPVRDLTFCPGCPHRASFWSIHNVLAADNREGFVCGDIGCYSMA 423 Query: 418 VLPP-LRTVDTTVAMGASIGIGHGLSIAMNGSLAEEEHKEGKEKQIIVATIGDSTFYHTG 476 LP T+ T AMG+ G+ G L Q +V+ GDSTF+H+G Sbjct: 424 FLPSGFSTLKTLHAMGSGTGVASGFGKLGQFGL----------DQPVVSVCGDSTFFHSG 473 Query: 477 LPALANAIYNRSNVLIVVLDNLVTAMTGDQPNPGTGQTPHG-MGKRIPIEDVAKAMGADF 535 +PAL NAI+++S++ +V+LDN T MTG Q +PG G + I + +A+GA Sbjct: 474 IPALVNAIHHKSDITMVILDNSGTGMTGFQSHPGLPVDAQGNESPALDIPTICRALGAT- 532 Query: 536 VAVVDPYDIKATYETIKKALEVEGVSVVVSRQVCALYKIGQMRRRGMKWPIYHVVEDKCT 595 V V DP+ T +T K LE G V+V RQ+CAL ++G K V ED C Sbjct: 533 VEVCDPFYPYKTQQTFHKLLEAPGAKVLVLRQICAL----SPEKKGKKQFEMSVDEDVCL 588 Query: 596 G--C---KICINAYGCPAIYWDPETKKAKVDPTMCWGCGGCAQVCPFDA 639 G C K+C + CP + WD E K A +D +C GCG CA +CP A Sbjct: 589 GENCGCNKLCTRVFRCPGLRWDEEKKVATIDEVICAGCGMCAAICPSGA 637 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1016 Number of extensions: 45 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 647 Length of database: 644 Length adjustment: 38 Effective length of query: 609 Effective length of database: 606 Effective search space: 369054 Effective search space used: 369054 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory