Align alpha-ketoglutarate TRAP transporter, 4TM/12TM components (characterized)
to candidate WP_028316487.1 G491_RS0125035 DUF3394 domain-containing protein
Query= reanno::psRCH2:GFF84 (674 letters) >NCBI__GCF_000429905.1:WP_028316487.1 Length = 683 Score = 351 bits (901), Expect = e-101 Identities = 211/567 (37%), Positives = 318/567 (56%), Gaps = 57/567 (10%) Query: 44 RAGHVGFLLLLVFLCYPARGNGK------------PFQPVAWLLGLAGFATFFYQWYFEA 91 RA H+ F + L+FL P + P A L +A A+ + ++ Sbjct: 59 RAFHLAFAIALLFLLTPLMRKARKGMEYFFVTDRIPLMDYA-LAVIAVIASLYIVIDYDG 117 Query: 92 DLIQRSGDMTTADMVVGLTLIVLVFEAARRVMGIALPIICALFLAYGLLGEYLPGDLAHR 151 L R+G T D+ G+ L+V++ EAARRV+G AL II +F Y LG Y+P LA + Sbjct: 118 -LAYRAGMPITRDIAFGVVLVVVLLEAARRVIGPALSIIAIVFTVYAFLGPYMPDFLAFK 176 Query: 152 GYYLDQIVNQLSFGTEGLYGTPTYVSATYIFLFILFGSFLEQAGMIKLFTDFAMGLFGHK 211 G L + + +S TEG+YG P VSA+ ++LF+L G+ L++AG + FTD A+ G Sbjct: 177 GVSLRKYCSNISLSTEGIYGIPLGVSASIVYLFVLLGALLDKAGAGQFFTDLALSFLGKY 236 Query: 212 LGGPAKVSVVSSALMGTITGSGVANVVTTGQFTIPLMKRFGYRPAFAGGVEATSSMGSQI 271 GGPAK +VV+S G ++GS +AN+VTTG FTIPLMK+ GY A VE SS Q+ Sbjct: 237 KGGPAKAAVVASGATGLVSGSSIANIVTTGPFTIPLMKKVGYPAKKAAAVEVASSTDGQL 296 Query: 272 MPPVMGAVAFIMAETINVPFVEIAKAALIPALLYFGSVFWMVHLEAKRAGLKGLPKDECP 331 MPP+MGA AFI+AE +NVP++E+ KAA IPA + +F + HLEA + G+ GL K E P Sbjct: 297 MPPIMGAAAFIIAEYVNVPYIEVVKAAAIPAFASYFGLFCITHLEASKLGISGLAKHEVP 356 Query: 332 SAMAAVKERWYLLIPLVVLVW-LLFSGRTPMFAGTIGLALTAIVILGSAIILKVSNFALR 390 +K + L+PL VL++ L+ +P A A AI+IL I + LR Sbjct: 357 VFFQTLKGGLHYLLPLGVLLYELIILRHSPEMA-----AFRAILILFVVIFYQ----ELR 407 Query: 391 IAFWIALGLLCAGFFQLGIGVIFGVIAALVAVCWFIKGGRDTLVICLHALVEGARHAVPV 450 +F GIG +G D++ I +++G+++ +PV Sbjct: 408 RSFKAG----------TGIG----------------QGFMDSIRIIGMGMIQGSKNMMPV 441 Query: 451 GIACALVGVIIGVVSLTGVASTFAGYILAVGENNLFLSLLLTMLTCLVLGMGIPTIPNYI 510 +ACA G+I+GVV++ G+ A + + + N++L LL+T + L+LGMG+PT YI Sbjct: 442 ALACAAAGIIVGVVNM-GIGGMVAQIVETLSQGNIYLLLLITAIASLLLGMGLPTTATYI 500 Query: 511 ITSSIAAPALLDLG------VPLIVSHMFVFYFGIMADLTPPVALACFAAAPIAKERGLK 564 + +S+ AP ++ +G +PLI +H+F FYFGI+AD TPPV LA +AA+ IA + Sbjct: 501 VMASLTAPIIVQVGGNFGFVLPLIAAHLFCFYFGILADDTPPVGLAAYAASAIADSDPIP 560 Query: 565 ISMWAIRIAIAGFIVPFMAVYNPALMM 591 + + I ++ F+ V+NP L++ Sbjct: 561 TGIQGFKYDIRTSVIAFLFVFNPDLLL 587 Lambda K H 0.330 0.143 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1253 Number of extensions: 73 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 674 Length of database: 683 Length adjustment: 39 Effective length of query: 635 Effective length of database: 644 Effective search space: 408940 Effective search space used: 408940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory