Align methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_028584323.1 G494_RS0109360 methylmalonyl-CoA mutase
Query= BRENDA::P11653 (728 letters) >NCBI__GCF_000429965.1:WP_028584323.1 Length = 717 Score = 803 bits (2073), Expect = 0.0 Identities = 415/700 (59%), Positives = 519/700 (74%), Gaps = 18/700 (2%) Query: 27 ELAAKAGTGEA-----WETAEQIPVGTLFNEDVYKDMDWLDTYAGIPPFVHGPYATMYAF 81 E+A K G++ W+T E I V L+ + M+ + + G+ P+ GP ATMYA Sbjct: 12 EVATKQMKGKSPDTLTWKTPEGIDVKCLYTAADIEGMETVGSMPGMAPYARGPMATMYAG 71 Query: 82 RPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMAGV 141 RPWTIRQYAGFSTA ESNAFYRRNLAAGQKGLSVAFDL THRGYDSDNPRVAGD+G AGV Sbjct: 72 RPWTIRQYAGFSTAAESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDNPRVAGDIGKAGV 131 Query: 142 AIDSIYDMRELFAGIPLDQMSVSMTMNGAVLPILALYVVTAEEQGVKPEQLAGTIQNDIL 201 AIDSI DM+ LF GIPLD+MSVSMTMNGAV+PIL +++ AEE GVK EQL GTIQNDIL Sbjct: 132 AIDSIEDMKILFDGIPLDKMSVSMTMNGAVIPILGMWIAAAEESGVKQEQLMGTIQNDIL 191 Query: 202 KEFMVRNTYIYPPQPSMRIISEIFAYTSANMPKWNSISISGYHMQEAGATADIEMAYTLA 261 KE++ RNTYIYPP PSMRI+S+I + S NMPK+N+ISISGYH+ EAGA + ++ A+TLA Sbjct: 192 KEYLTRNTYIYPPTPSMRIVSDIMEFASQNMPKYNTISISGYHIMEAGADSVLQTAFTLA 251 Query: 262 DGVDYIRAGESVGLNVDQFAPRLSFFWGIGMNFFMEVAKLRAARMLWAKLVHQFGPKNPK 321 DGV+Y+RA + G++VD FAPRLSFF+GIGMNFFM++A LRAAR LW +L+ QF P+NPK Sbjct: 252 DGVEYVRAAIASGMSVDTFAPRLSFFFGIGMNFFMDIAMLRAARFLWHRLMQQFNPENPK 311 Query: 322 SMSLRTHSQTSGWSLTAQDVYNNVVRTCIEAMAATQGHTQSLHTNSLDEAIALPTDFSAR 381 S LRTH QTSGWSLT QD YNNV+RT +EAM+A G TQSLHTNS DEA+ LPTDFSAR Sbjct: 312 SSMLRTHCQTSGWSLTEQDPYNNVIRTTMEAMSAVLGGTQSLHTNSYDEAVCLPTDFSAR 371 Query: 382 IARNTQLFLQQESGTTRVIDPWSGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIEKGI 441 IARNTQ+ +Q+ES +V DP GS Y+E LT + +A I E+E++GGMAKAIE G+ Sbjct: 372 IARNTQIIIQEESHVCKVADPLGGSYYIEYLTNQIVDEAQKIIDEIEEIGGMAKAIESGM 431 Query: 442 PKMRIEEAAARTQARIDSGRQPLIGVNKYRLEHEP-PLDVLKVDNSTVLAEQKAKLVKLR 500 PKMRIEE+AAR QARID G ++GVNKYRLE E D+L+V + TV EQ ++ ++R Sbjct: 432 PKMRIEESAARRQARIDQGSDVIVGVNKYRLEDEKLDFDILEVPD-TVRTEQIDRINEIR 490 Query: 501 AERDPEKVKAALDKITWAAGNPDDKDPDRNLLKLCIDAGRAMATVGEMSDALEKVFGRYT 560 R+ + +A L+ +T AA + NLL++ I A +A ATVGE+SDA+EKVFGRY Sbjct: 491 KTRNEAECQACLEAVTKAA------EGKGNLLEVAIAAAKARATVGEISDAMEKVFGRYV 544 Query: 561 AQIRTISGVYSKEV-----KNTPEVEEARELVEEFEQAEGRRPRILLAKMGQDGHDRGQK 615 A + +SG YS E ++ +E+ R+ F + GRRPRIL+ KMGQDGHDRG K Sbjct: 545 ATTQCVSGAYSSEYAEADSESAQVIEDFRKRTGNFLEKTGRRPRILVTKMGQDGHDRGIK 604 Query: 616 VIATAYADLGFDVDVGPLFQTPEETARQAVEADVHVVGVSSLAGGHLTLVPALRKELDKL 675 V+A+++ADLGFDVD+ P+FQTP+E A+ A+E DVH+VG SSLA GH TLVP L +L + Sbjct: 605 VVASSFADLGFDVDISPMFQTPDEAAKMAIENDVHIVGASSLAAGHKTLVPELIAKLKEQ 664 Query: 676 GRPDILITVGGVIPEQDFDELRKDGAVEIYTPGTVIPESA 715 G +I + GGVIP D+D L + G ++ PGT I SA Sbjct: 665 GAGEIGVVAGGVIPPSDYDFLYEAGTKAVFGPGTPITTSA 704 Lambda K H 0.317 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1247 Number of extensions: 37 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 728 Length of database: 717 Length adjustment: 40 Effective length of query: 688 Effective length of database: 677 Effective search space: 465776 Effective search space used: 465776 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory