Align phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) (EC 5.4.2.2) (characterized)
to candidate WP_028585974.1 G494_RS0120225 phosphomannomutase/phosphoglucomutase
Query= BRENDA::Q8PGN7 (450 letters) >NCBI__GCF_000429965.1:WP_028585974.1 Length = 457 Score = 536 bits (1382), Expect = e-157 Identities = 266/459 (57%), Positives = 334/459 (72%), Gaps = 11/459 (2%) Query: 1 MPMTLPAFKAYDIRGRVPDELNEDLARRIGVALAAQLDQGPVVLGHDVRLASPALQEALS 60 M ++ F AYDIRG+VPD+L+E+LA IG+A +VLGHD+RL SPA+ AL+ Sbjct: 1 MSDSVRCFTAYDIRGKVPDDLDEELAALIGLAFIEHFAAKKIVLGHDMRLTSPAISRALT 60 Query: 61 AGLRASGRDVIDIGLCGTEEVY---FQTDYLKAAGGVMVTASHNPMDYNGMKLVREQARP 117 + A G DV+DIGLCGTEEVY F GG+M+TASHNP YNGMKLV+ +ARP Sbjct: 61 RAVLARGCDVVDIGLCGTEEVYHRVFAGADQGVDGGIMITASHNPAAYNGMKLVQREARP 120 Query: 118 ISSDTGLFAIRDTVAADT------AAPGEPTASEQSRTDKTAYLEHLLSYVDRSTLKPLK 171 +S ++GL I V+ T G P S + +K Y++HLLS VD LKPLK Sbjct: 121 VSGESGLAEIEARVSDTTWRRQAGKDSGVPPGSYRLEEEKGGYIQHLLSSVDLEQLKPLK 180 Query: 172 LVVNAGNGGAGLIVDLLAPHLPFEFVRVFHEPDGNFPNGIPNPLLPENRDATAKAVKDNG 231 LVVN GNG AG I+DLL HLPF F+R+ +PDG+FPNG+PNPLLPE R TA+AV+++G Sbjct: 181 LVVNPGNGCAGPIIDLLESHLPFTFIRLQEQPDGSFPNGVPNPLLPEKRAVTAEAVREHG 240 Query: 232 ADFGIAWDGDFDRCFFFDHTGRFIEGYYLVGLLAQAILAKQPGGKVVHDPRLTWNTVEQV 291 AD GIAWDGDFDRCFF+D TGRFIEGYY+VGLL AIL + PG ++HDPRLTWNT+E V Sbjct: 241 ADLGIAWDGDFDRCFFYDETGRFIEGYYIVGLLGTAILQQHPGATILHDPRLTWNTIEMV 300 Query: 292 EEAGGIPVLCKSGHAFIKEKMRSENAVYGGEMSAHHYFREFAYADSGMIPWLLIAELVSQ 351 E AGG P++ ++GHAFIKE MR+ + VYGGEMSAHHYFR+F Y DSGM+PWLL+ +L+S Sbjct: 301 EAAGGHPLMTRTGHAFIKENMRAADGVYGGEMSAHHYFRDFGYCDSGMVPWLLVTQLMSS 360 Query: 352 SGRSLADLVEARMQKFPCSGEINFKVADAKASVARVMEHYASLSPELDYTDGISADFGQW 411 SG+ L+ +V+ RMQ+FP SGEIN VAD + +V E Y ELD+TDGIS + ++ Sbjct: 361 SGQPLSAMVDDRMQRFPVSGEINSTVADPDGIIRQVEERYR--DGELDHTDGISVSYPEY 418 Query: 412 RFNLRSSNTEPLLRLNVETRGDAALLETRTQEISNLLRG 450 RFN+R SNTEP+LRLNVETRGDAALL T+E+ L+RG Sbjct: 419 RFNIRKSNTEPVLRLNVETRGDAALLHRTTEELLALIRG 457 Lambda K H 0.319 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 676 Number of extensions: 32 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 450 Length of database: 457 Length adjustment: 33 Effective length of query: 417 Effective length of database: 424 Effective search space: 176808 Effective search space used: 176808 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory