Alignments for a candidate for dauA in Sedimenticola selenatireducens DSM 17993

GapMind for catabolism of small carbon sources

Alignments for a candidate for dauA in Sedimenticola selenatireducens DSM 17993

Align C4-dicarboxylic acid transporter DauA; Dicarboxylic acid uptake system A (characterized)
to candidate WP_029131733.1 A3GO_RS0100480 C4-dicarboxylic acid transporter DauA

Query= SwissProt::P0AFR2
         (559 letters)



>NCBI__GCF_000428045.1:WP_029131733.1
          Length = 563

 Score =  504 bits (1298), Expect = e-147
 Identities = 261/549 (47%), Positives = 383/549 (69%), Gaps = 14/549 (2%)

Query: 12  FRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIV 71
           F AL  A   E+ + +    +++AG+TVG+IA+PL+MALAI SGV PQ+GLYTA VAGIV
Sbjct: 2   FSALRTALVNER-SLSSIQSNILAGLTVGVIALPLSMALAIASGVPPQHGLYTAIVAGIV 60

Query: 72  IALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYI 131
           IALTGGS+ ++SGPTAAFVV+L P+ QQ+G+ GLLV+  L+GI L+L+GLAR GRLIE +
Sbjct: 61  IALTGGSKVNISGPTAAFVVVLLPIVQQYGIGGLLVSGFLAGIILVLLGLARLGRLIEIV 120

Query: 132 PVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPTINVGDAAIGIV 191
           P  VT+GFT+GIG+ I T QIKDFLGL +  +  HY+ K+  +  ++P+IN  +  IG +
Sbjct: 121 PYPVTVGFTAGIGVVIATFQIKDFLGLNIQSLDGHYIDKLWLILQSVPSINWQETMIGSL 180

Query: 192 TLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGG--HVATIGSQFHYVLADGSQGNG 249
           +LG+L+ WPRL  R+PGHL ALL G     +++ +     VATIGS+FHY L +G  G+G
Sbjct: 181 SLGVLILWPRLQSRIPGHLVALLIGSVAAWLISQITSDFSVATIGSRFHYEL-NGITGSG 239

Query: 250 IPQLLPQLVLPWDLPNSE---FTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGT 306
           IP  LP    PW+LP++E      ++  + TLL +A ++A+LG++ESLLCAVV DGM+G 
Sbjct: 240 IPPFLPGFEWPWNLPDAEGNSIGFSFSLLNTLLASAITIAILGSLESLLCAVVADGMSGK 299

Query: 307 KHKANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLV 366
           KH  N EL+GQG+GN++A  FGGI ATAAIAR+AANVRAG TSP+++V+H + +++A+L 
Sbjct: 300 KHNPNDELIGQGIGNMVASLFGGIPATAAIARTAANVRAGGTSPLASVVHGLFILVAILS 359

Query: 367 LAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIA 426
           L+P+L+++P+++MAALLLMVAWNMSEA   +  L+ AP+DDI+ ++ C  LTVLFDM IA
Sbjct: 360 LSPVLAYIPMASMAALLLMVAWNMSEAKHFIRTLKIAPRDDILTLVTCFLLTVLFDMTIA 419

Query: 427 ISVGIVLASLLFMRRIARMTRLAPV------VVDVPDDVLVLRVIGPLFFAAAEGLFTDL 480
           + VG+ LA++LF+ R  R+T    V       +++P  + +  + GPLFF +A+    ++
Sbjct: 420 VGVGMGLAAMLFIHRSIRLTENTKVESGRMNELELPSQISIYDINGPLFFGSAQKALKNI 479

Query: 481 ESRLEGKRIVILKWDAVPVLDAGGLDAFQRFVKRL-PEGCELRVCNVEFQPLRTMARAGI 539
            +     R+++L    V ++D   + A +  V  L  +   L + N++ + +  + RAG+
Sbjct: 480 TAITPEVRVIVLDMTEVTMVDMSAIVAMESIVANLAAKNVGLVISNLQPRIILKLRRAGV 539

Query: 540 QPIPGRLAF 548
           +  PGR+ F
Sbjct: 540 RTRPGRIRF 548


Lambda     K      H
   0.328    0.142    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 727
Number of extensions: 28
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 563
Length adjustment: 36
Effective length of query: 523
Effective length of database: 527
Effective search space:   275621
Effective search space used:   275621
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources