Align Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 (characterized)
to candidate WP_029132303.1 A3GO_RS0103980 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= SwissProt::Q99MR8 (717 letters) >NCBI__GCF_000428045.1:WP_029132303.1 Length = 660 Score = 525 bits (1351), Expect = e-153 Identities = 293/675 (43%), Positives = 421/675 (62%), Gaps = 28/675 (4%) Query: 47 KVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAM 106 K+LIANRGEIACRVI++A+KMG+++VAV+S+ADR ++HV MADEA IGP+ S +SYL M Sbjct: 4 KILIANRGEIACRVIKSARKMGIKTVAVFSDADREALHVKMADEAVHIGPSASAESYLVM 63 Query: 107 EKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGIKSTSKSIM 166 E+IIQ + + AQA+HPGYGFLSEN F E ++EGI FIGP + AI MG K TSK++ Sbjct: 64 ERIIQACRDTGAQAVHPGYGFLSENSAFCEALEKEGIAFIGPATLAINSMGDKITSKNLA 123 Query: 167 AAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSEREFQEQLES 226 AGV V+ GY + + E + IGYPVM+KA GGGGKGMR+ +E E ++ E Sbjct: 124 EQAGVNVIPGYTDVVRDAEHAVEISNGIGYPVMLKASAGGGGKGMRVAWNEAECRDGFER 183 Query: 227 ARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAP 286 A EA+ SF DD + IEKF++ PRH+E+QV D HGN +YL ER+CS+QRRHQK+IEEAP Sbjct: 184 ATNEARSSFGDDRVFIEKFIEEPRHIEIQVMADTHGNVIYLGERECSIQRRHQKVIEEAP 243 Query: 287 APGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDSRHNFYFMEMNTRLQVEHPVTEMI 346 +P I+ R+++GE AV+ A+AV+Y AGTVEFI+D++ NFYF+EMNTRLQVEHPVTE++ Sbjct: 244 SPFIDEATRKQMGEQAVQLARAVQYRSAGTVEFIVDAKRNFYFLEMNTRLQVEHPVTELV 303 Query: 347 TGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPSA 406 TG DLVE +R+A GE++PL QE++ L G A E+R+YAEDP NF+P G LV P Sbjct: 304 TGQDLVELMIRVANGEELPLRQEDVKLTGWAMESRVYAEDPFRNFLPSIGRLVKYEPPVG 363 Query: 407 DMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIVGLRSNVDFL 466 D + R++TGV +G EVS+ YDPMIAKL+ + ++R A+++++ L Y I G++ N+ FL Sbjct: 364 D-NVRVDTGVYEGGEVSMFYDPMIAKLITYGANRNEAITRMQDALDAYCIRGVQHNISFL 422 Query: 467 LRLSGHPEFEAGNVHTDFIPQHHKDLLPSHSTIAKESVCQAALGLILKEKEMTSAFKLHT 526 L HP F G + T+FI + D + + A+ ++ + M A +L Sbjct: 423 NALMVHPRFIEGRLTTNFIAEEFPDGFDASYVPQDDPAKVVAVAAMIHRQYMDRAARLSG 482 Query: 527 QDQFSPFSFSSGRRLNISYTRNMTLRSGKSDIVIAVTYNRDGSYDMQIDNKSFRVLGDLS 586 Q + R++N + + + +G+ V AV N G ++ + V + + Sbjct: 483 QLR------GHERKVNDEW---VVIINGEHHAVSAVPAN--GGCRVEYAGNGYDVTTEWA 531 Query: 587 SEDGCTYLKSSING------VARKSKFILL----DNTVHLFSMEGSIEVGIPVPKYLSPV 636 G L +ING V RK ++ L + T L + E+ +P+ +P Sbjct: 532 L--GQPLLHGTINGLSVCFQVERKGLWLRLFHRGNQTDALVMTPRAAELHALMPEKEAPD 589 Query: 637 SAEGAQGGTIAPMTGTIEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGRIKKVFFS 696 ++ ++PM G + KV V G VKAG+ L V+ AMKME+ ++A +D + + Sbjct: 590 LSK----FLLSPMPGLLVKVSVVEGQEVKAGEELAVIEAMKMENVLRAEQDATVASIQAE 645 Query: 697 EGAQANRHAPLVEFE 711 G ++EFE Sbjct: 646 AGDSLRVDQVIIEFE 660 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1099 Number of extensions: 47 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 717 Length of database: 660 Length adjustment: 39 Effective length of query: 678 Effective length of database: 621 Effective search space: 421038 Effective search space used: 421038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory