Align Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate WP_029133951.1 A3GO_RS0114180 ABC transporter ATP-binding protein
Query= uniprot:G8ALJ1 (236 letters) >NCBI__GCF_000428045.1:WP_029133951.1 Length = 235 Score = 239 bits (609), Expect = 4e-68 Identities = 121/235 (51%), Positives = 170/235 (72%), Gaps = 1/235 (0%) Query: 1 MLKVSGVHTFYGAIEALKGVDIEIGAGEIVSLIGANGAGKSTLLMTICGSPRARMGRITF 60 ML++ V +YG I+ALKG+++E+ GEI++LIGANGAGKST LM+I G R G++ + Sbjct: 1 MLELRNVDAYYGNIQALKGINLEVREGEIITLIGANGAGKSTTLMSISGIVPPRNGQVLY 60 Query: 61 EGQDITQMPTYELVRLGIAQSPEGRRIFPRMSVLENLQMGS-ITAKPGSFANELERVLTL 119 +G+ I + ++V +GI Q PEGR IFP+++V ENL MG+ + + +L+ V +L Sbjct: 61 KGEPIHNVAPDKIVAMGICQVPEGRHIFPQLTVQENLDMGAFLRSDKRGIQKDLDYVFSL 120 Query: 120 FPRLKERISQRAGTMSGGEQQMLAIGRALMSQPRLLLLDEPSLGLAPLVVKQIFQAVKDI 179 FP L ER Q G +SGGEQQMLA+ RALM++P LLLLDEPS+GLAPL+++QIF+ +K I Sbjct: 121 FPILDERRHQAGGNLSGGEQQMLAMSRALMARPDLLLLDEPSMGLAPLIIRQIFEIIKKI 180 Query: 180 NREQKMTVFMVEQNAFHALKLAHRGYVMVNGKVTMSGTGAELLANEEVRSAYLEG 234 N E T+F+VEQNA AL +A RGYV+ NG +T+S +LLAN++V+ AYL G Sbjct: 181 NDENNTTIFLVEQNANQALHIADRGYVIENGSITISDDADKLLANQDVQKAYLGG 235 Lambda K H 0.320 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 175 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 236 Length of database: 235 Length adjustment: 23 Effective length of query: 213 Effective length of database: 212 Effective search space: 45156 Effective search space used: 45156 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory