Alignments for a candidate for boxB in Sedimenticola selenatireducens DSM 17993

GapMind for catabolism of small carbon sources

Alignments for a candidate for boxB in Sedimenticola selenatireducens DSM 17993

Align Benzoyl-CoA oxygenase component B; Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component; EC 1.14.13.208 (characterized)
to candidate WP_029134685.1 A3GO_RS0118600 benzoyl-CoA 2,3-epoxidase subunit BoxB

Query= SwissProt::Q9AIX7
         (473 letters)



>NCBI__GCF_000428045.1:WP_029134685.1
          Length = 481

 Score =  625 bits (1611), Expect = 0.0
 Identities = 294/472 (62%), Positives = 367/472 (77%), Gaps = 3/472 (0%)

Query: 2   INYSERIPNNVNLNENKTLQRALEQWQPSFLNWWDDMGPENSSNYDVYLRTAVSVDPKGW 61
           +NY+++IPNNVNL+E++ LQRA+E+WQP F++WW  MGP+   + +VYLRTAV  DPKGW
Sbjct: 7   VNYADKIPNNVNLSEDRRLQRAMEKWQPEFIDWWKSMGPDGFQDAEVYLRTAVGADPKGW 66

Query: 62  ADFGYVKMHDYRWGIFLAPQEGEKKITFGEHKGQDVWQEVPGEYRSTLRRIIVTQGDTEP 121
           A F YVKM +YRWGI LA +E  + I FG+HKG+  WQEVPGEYRS L+R+IV QGDTEP
Sbjct: 67  AVFDYVKMPEYRWGILLAAKEEGRVIPFGKHKGEPAWQEVPGEYRSMLKRLIVIQGDTEP 126

Query: 122 ASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVYLLHAHFGRDGREEGEALLERRS 181
           ASVEQQR+LG T PSLYDLRNLFQVNVEEGRHLWAM YLL  +FG+DGREE EALL+RRS
Sbjct: 127 ASVEQQRYLGKTCPSLYDLRNLFQVNVEEGRHLWAMAYLLQKYFGKDGREEAEALLDRRS 186

Query: 182 GDEDNPRILTAFNEKTPDWLSFFMFTFITDRDGKFQLASLAESAFDPLARTCKFMLTEEA 241
           GDED PRIL AFNEKTPDWLSFFMFTF TDRDGK QL SLA+S FDPLARTC+FMLTEEA
Sbjct: 187 GDEDKPRILGAFNEKTPDWLSFFMFTFFTDRDGKMQLESLAQSGFDPLARTCRFMLTEEA 246

Query: 242 HHLFVGESGIARVIQRTCEVMKELGTDDPAK---LRAAGVIDLPTLQKYLNFHYSVTSDL 298
           HH+FVGESG++RVI++T E M+E G  DP +   +RA GV+DLPTLQ+ +NFH+SVT DL
Sbjct: 247 HHMFVGESGVSRVIRKTLERMQEAGITDPNEVDAVRALGVVDLPTLQRKVNFHFSVTLDL 306

Query: 299 YGAEISSNAATYYTNGLKGRFEEEKIGDDHKLQNSEYEVMDVAGDKILTRHVPALSALNE 358
           +GAE+S+NAA  +  G+KGRF E++I DDH+L N  Y V+     ++      AL+A+N 
Sbjct: 307 FGAEVSTNAANAFNAGIKGRFREDRIEDDHQLLNDTYPVLRHVDGELKVVEETALNAINA 366

Query: 359 RLRDDWITDVQAGVDRWNRIPAKFGFDFRFTLPHKGFHRKIGMFADVHVSPDGRLISEAE 418
           RL DD+ITD  AGV RWN++    G DF  ++PHK F+R++G FAD+HV P+G L+S   
Sbjct: 367 RLLDDYITDATAGVKRWNKLFEGSGVDFEISVPHKAFNRRVGEFADLHVDPEGNLLSAEA 426

Query: 419 WTHQHKNWLPTESDRLYVHSLMGRCLEPGKFANWIAAPARGINNQPVNFEYV 470
           W  +   WLP+++D  Y+ SLM    E GK+A+WIA P  GI+ +P +FEYV
Sbjct: 427 WQEKSAQWLPSQADEDYLLSLMKPVTEKGKYADWIAPPKVGIDKKPGDFEYV 478


Lambda     K      H
   0.320    0.137    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 809
Number of extensions: 23
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 473
Length of database: 481
Length adjustment: 33
Effective length of query: 440
Effective length of database: 448
Effective search space:   197120
Effective search space used:   197120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources