Align indolepyruvate ferredoxin oxidoreductase (subunit 2/2) (EC 1.2.7.8) (characterized)
to candidate WP_035235383.1 Q366_RS00625 indolepyruvate oxidoreductase
Query= BRENDA::O07835 (647 letters) >NCBI__GCF_000745975.1:WP_035235383.1 Length = 604 Score = 361 bits (927), Expect = e-104 Identities = 227/630 (36%), Positives = 351/630 (55%), Gaps = 46/630 (7%) Query: 18 LLLGNHAIARGALEANIAVFAAYPGTPSSELTDTMAAVAKKAG--VYMEYSTNEKVAFET 75 L+LGN A+A G L+ + AYPGTPSSE+ + ++ K+ ++ +++ NEKVAFET Sbjct: 6 LILGNEALAYGLLKNGCQMACAYPGTPSSEILSAVVSLKKEMDLDIHAQWAVNEKVAFET 65 Query: 76 ALAAAWSGLRAMTAMKHVGLNVAADSFLSSVGMGVEGGFVIMVADDPSMWSSQNEQDTRV 135 A A A +GLR AMK VGLNVAAD +SSV +GV+GGF+++ ADDP SSQ EQD+R+ Sbjct: 66 AYAGAQAGLRTAVAMKQVGLNVAADPLMSSVYLGVKGGFLVISADDPGPHSSQTEQDSRL 125 Query: 136 YAKFANVPVLEPSSPHEAKEMTKYAFELSEKFKHFVILRTTTRSSHARGDVVLGELPEEI 195 A A +PVL+P SP +A E+ AFELSE F+ V+LR TTR H+R + + E+I Sbjct: 126 MAVMAKLPVLDPDSPGQAAELAGIAFELSEAFEIPVMLRPTTRVCHSRQSMDV----EKI 181 Query: 196 KTGKRKFGKFKKDPTRFVDVPAHARKFHPLILEKIEKIREELNNCPFNWIEGKEDAKVGI 255 + +R+ F K+P R+ P + H + K+ KI + P + I Sbjct: 182 EMTRRE-AAFDKNPGRWAATPKFRLQLHKELEAKLTKIADYEPTRPRLVSGTAKGCGQAI 240 Query: 256 IAPGLSYAYVKEALAWLGVEDVKILKLGTPFPVPYGLLGKFFDGLEKVLIVEELEPVVEE 315 + G++ A ++ + + D+ + ++ PFP+ + + + +++L++EE V+E Sbjct: 241 VVAGVAAANARDIIKERSL-DIPVYQVVQPFPLHKEFI-RQMNAYDEILVLEETWGVIEM 298 Query: 316 QVKTWAYDKGLRIPIHGKDLVPRVYEMTTRRAVEAIAKFLGLETPINFAEIDEKYEKVSQ 375 Q+ DK R+ + V E+ E I F G +D + +++ Sbjct: 299 QLA----DKN-RVKGKNTGFISPVGELLPENVEERICAFAG---------VDYQAPQIT- 343 Query: 376 IVPPRPPSLCPACPHRNSFFAIRKAAGPKAIYPSDIGCYTLGVLPPLRTVDTTVAMGASI 435 ++P R P+LC CPHR SF+AI+KAA PK I+ SDIGCYTLG L VDT MGA I Sbjct: 344 MLPGRRPTLCAGCPHRASFYAIKKAA-PKGIFTSDIGCYTLGC--NLGAVDTVTCMGAGI 400 Query: 436 GIGHGLSIAMNGSLAEEEHKEGKEKQIIVATIGDSTFYHTGLPALANAIYNRSNVLIVVL 495 G +IA + + +++ + +TIGDSTF+H+G+P L + + ++V+L Sbjct: 401 SQAAGFTIA---------YAKNEKQPPVFSTIGDSTFFHSGIPGLIETVTKKIPFVLVIL 451 Query: 496 DNLVTAMTGDQPNPGTGQTPHGMG-KRIPIEDVAKAMGADFVAVVDPYDIKATYETIKKA 554 DN TAMTG QP P +G+ G + I + K G +F+ + DPYD+ A + +K+A Sbjct: 452 DNRTTAMTGHQPTPASGRDASGDSCIAVNIPAIVKGCGVNFIKIADPYDLPAFIDILKEA 511 Query: 555 ---LEVEGVSVVVSRQVCALYKIGQMRRRGMKWPIYHVV--EDKCTGCKICINAYGCPAI 609 + G +VV++ C L M R +K V+ +D C GC C++ + CPA+ Sbjct: 512 KAYCKENGPAVVIAEHPCLL----DMDRAELKASFKKVIVNKDICDGCGYCVSHFECPAL 567 Query: 610 YWDPETKKAKVDPTMCWGCGGCAQVCPFDA 639 + +T++ +DP +C GC C+ VCP DA Sbjct: 568 GLNNDTEQVCIDPGLCTGCAVCSFVCPKDA 597 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 945 Number of extensions: 44 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 647 Length of database: 604 Length adjustment: 38 Effective length of query: 609 Effective length of database: 566 Effective search space: 344694 Effective search space used: 344694 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory