Protein WP_035629258.1 in Hydrogenovibrio marinus DSM 11271
Annotation: NCBI__GCF_000711315.1:WP_035629258.1
Length: 550 amino acids
Source: GCF_000711315.1 in NCBI
Candidate for 15 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-mannose catabolism | TM1750 | med | TM1750, component of Probable mannose/mannoside porter. Induced by beta-mannan (Conners et al., 2005). Regulated by mannose-responsive regulator manR (characterized) | 50% | 79% | 260.8 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-mannose catabolism | TM1749 | med | TM1749, component of Probable mannose/mannoside porter. Induced by beta-mannan (Conners et al., 2005). Regulated by mannose-responsive regulator manR (characterized) | 41% | 81% | 191.4 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-cellobiose catabolism | TM0027 | lo | TM0027, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized) | 39% | 91% | 170.6 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-histidine catabolism | PA5503 | lo | Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) | 36% | 72% | 160.2 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-cellobiose catabolism | cbtD | lo | CbtD, component of Cellobiose and cellooligosaccharide porter (characterized) | 35% | 71% | 159.8 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-cellobiose catabolism | TM0028 | lo | TM0028, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized) | 36% | 83% | 150.2 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-proline catabolism | proV | lo | Glycine betaine/proline betaine transport system ATP-binding protein ProV (characterized) | 33% | 64% | 147.9 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-proline catabolism | opuBA | lo | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) | 35% | 59% | 147.1 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-histidine catabolism | hutV | lo | HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) | 34% | 85% | 138.7 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-proline catabolism | hutV | lo | HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) | 34% | 85% | 138.7 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-arginine catabolism | artP | lo | Arginine transport ATP-binding protein ArtM (characterized) | 36% | 100% | 136 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-histidine catabolism | hisP | lo | Histidine transport ATP-binding protein HisP (characterized) | 33% | 96% | 134.8 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| L-lysine catabolism | hisP | lo | Histidine transport ATP-binding protein HisP (characterized) | 33% | 96% | 134.8 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-cellobiose catabolism | cbtF | lo | CbtF, component of Cellobiose and cellooligosaccharide porter (characterized) | 31% | 79% | 130.2 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
| D-glucosamine (chitosamine) catabolism | AO353_21725 | lo | ABC transporter for D-glucosamine, ATPase component (characterized) | 34% | 91% | 125.9 | uncharacterized ABC transporter ATP-binding protein yejF | 47% | 479.9 |
Sequence Analysis Tools
View WP_035629258.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MSSNQPVLKVENLCVELEKNHRPLISNVSFEIKAGEIFALVGESGSGKSLTSLAIMRLLP
EALQVSSGAISLNHQGLFSLPEYQMQKVRGRQVAMIFQEPMTSLNPVMKVGDQVAEVLRV
HLGMNRKTAREKVVSLFDEVGIPNPEERYDWYPHQLSGGQKQRVMIAMALACEPDLLIAD
EPTTALDVTIQAQVLNLLKQIRDDRGLAILFITHDMGVVNEIADRVAVMKDGEIVEQAET
GHFFTDPQHPYTQKLLSDAIPKRESFEDEALSTVQNLLQVNDLKVYFPIKKGIFQRTVGH
VKAVDGVNLTIPKGQTLALVGESGSGKSTIGQAILKLVDATAGQVNFTNDQAAQAINLVD
LSEKAMKPFRKKVQVIFQDPFSALNPRMTINEIIREGMNSLQVGPKSRVEQDKRIESLLE
QVGLLPEHKYRYPHEFSGGQRQRIGIARALAVEPELIICDEPTSALDVSVRAQVLALLKD
LQKTYQMSYLFITHDLSIVPSIAHHVAVMQAGKIVEQGLVEEVMQNPQHDYTQRLLASAP
KLVKESLISG
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory