Alignments for a candidate for N515DRAFT_2924 in Methylocapsa aurea KYG T

GapMind for catabolism of small carbon sources

Alignments for a candidate for N515DRAFT_2924 in Methylocapsa aurea KYG T

Align Basic amino acid/polyamine antiporter, APA family (characterized, see rationale)
to candidate WP_036259385.1 DL86_RS06255 amino acid permease

Query= uniprot:A0A1I1Y8J0
         (492 letters)



>NCBI__GCF_000746085.1:WP_036259385.1
          Length = 498

 Score =  483 bits (1242), Expect = e-141
 Identities = 246/500 (49%), Positives = 329/500 (65%), Gaps = 19/500 (3%)

Query: 2   LKNLFATTQISPASA---DLPGGGAHGEATLKRALTARHLVLLGIGAIIGAGIFVITGQA 58
           + N++A   ++   A   D   G     A L R L+   ++ LGIG IIGAGIFV+TG A
Sbjct: 1   MTNIWARKSMAVLEAEANDAEFGAGSETAGLHRTLSLASVIALGIGCIIGAGIFVLTGHA 60

Query: 59  AAEHAGPAIVLSFVFAGIACALAALCYAEFAAMLPVSGSAYSYSYATLGEYVAWFVGWSL 118
           AA +AGPA+ LSFV AGI CA A LCYAE A+ +PV+GSAY+Y+YAT+GE++AW +GW L
Sbjct: 61  AASYAGPAVSLSFVLAGIVCAFAGLCYAEMASTVPVAGSAYTYAYATMGEFIAWIIGWDL 120

Query: 119 VLEYLFTVATVAAGWSGYFNKLLALISGWIGHDVSLPQTLAAAP--FTVVDGHIQATGMF 176
           +LEY F   TVA GWSGY    L         D+++P  LA+AP  +   +G   +TG  
Sbjct: 121 ILEYAFGATTVAIGWSGYVTSFLK------DFDITIPAALASAPLAYDPANGDWTSTGAL 174

Query: 177 INLPAVAIIAAITGLCYVGITQSAFVNSIIVAIKVTVILLFIAFATKYINPDNW------ 230
            N+PA  II  +T L  VGI +SA VN+ IV IK+ +ILLFI      I+  NW      
Sbjct: 175 FNIPAAFIIVLLTVLLVVGIRESARVNNAIVLIKLAIILLFIVAGVSSISAANWVTSTNP 234

Query: 231 -HPFIPASEGASKYGWAGVGRAAAIVFFSYIGFDAVSTAAGEAKNPQRDMPIGIIGSLIL 289
              FIP + G  +YGW+G+ R AA+VFF+YIGFDAVSTAA EAKNPQRDMP+GI+GSL +
Sbjct: 235 SGAFIPPNLGPGQYGWSGIIRGAAVVFFAYIGFDAVSTAAQEAKNPQRDMPLGILGSLAI 294

Query: 290 CTILYIIVAGILTGIADFRLLGTPEPVSTALDNYPSLHWLQIIVVIGAVTGLSSVMLVML 349
           CT+LY++V  ++TG+  F  L  P+P++  +D    L WL  ++  GA+ GLSSV+LV+L
Sbjct: 295 CTVLYVLVGVVITGVVPFDKLNVPDPIALGVDAI-GLGWLSFLIKFGAILGLSSVILVLL 353

Query: 350 MGQPRIFYSMARDGLIPAVFGRIHQKFRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMG 409
           +GQPRIFYSMARDGL+P     +H +FRTP+V T++ G + A L GL  IG++GE+V++G
Sbjct: 354 LGQPRIFYSMARDGLLPPFAAMVHPRFRTPYVTTILTGAIVAILSGLLPIGLVGELVSIG 413

Query: 410 TLLAFATVCIGVLVLRYTRPELPRAFRVPVPWIVCPLGALACMALFLQSFLEHWRWMLAW 469
           TL AF  VC+GVLVLR T PE+ R F+ P  ++V PLGA + + L      + W  +  W
Sbjct: 414 TLFAFTVVCLGVLVLRITHPEIRRPFKTPFVFVVAPLGAASAIFLMFGLPSDTWLRLGIW 473

Query: 470 IAIGQAIYFLYGYSHSKLRK 489
           + IG AIYF YG  HS L +
Sbjct: 474 LVIGLAIYFFYGRQHSHLAR 493


Lambda     K      H
   0.328    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 831
Number of extensions: 45
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 492
Length of database: 498
Length adjustment: 34
Effective length of query: 458
Effective length of database: 464
Effective search space:   212512
Effective search space used:   212512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources