GapMind for catabolism of small carbon sources

 

Protein WP_038018808.1 in Thermithiobacillus tepidarius DSM 3134

Annotation: NCBI__GCF_000423825.1:WP_038018808.1

Length: 311 amino acids

Source: GCF_000423825.1 in NCBI

Candidate for 34 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism livG lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-leucine catabolism livG lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-phenylalanine catabolism livG lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-proline catabolism HSERO_RS00895 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-serine catabolism Ac3H11_1693 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-tyrosine catabolism Ac3H11_1693 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 100% 148.3 nodulation ATP-binding protein I 50% 310.8
L-fucose catabolism SM_b21106 lo ABC transporter for L-Fucose, ATPase component (characterized) 31% 89% 134 nodulation ATP-binding protein I 50% 310.8
N-acetyl-D-glucosamine catabolism SMc02869 lo N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 33% 76% 127.5 nodulation ATP-binding protein I 50% 310.8
D-glucosamine (chitosamine) catabolism SMc02869 lo N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 33% 76% 127.5 nodulation ATP-binding protein I 50% 310.8
L-isoleucine catabolism livF lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-leucine catabolism livF lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-phenylalanine catabolism livF lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-proline catabolism HSERO_RS00900 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-serine catabolism Ac3H11_1692 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-tyrosine catabolism Ac3H11_1692 lo ABC transporter ATP-binding protein (characterized, see rationale) 34% 95% 125.9 nodulation ATP-binding protein I 50% 310.8
L-arabinose catabolism xacJ lo Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) 32% 74% 124.8 nodulation ATP-binding protein I 50% 310.8
L-proline catabolism opuBA lo BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) (characterized) 31% 84% 120.6 nodulation ATP-binding protein I 50% 310.8
L-valine catabolism livG lo ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 31% 93% 119.8 nodulation ATP-binding protein I 50% 310.8
L-alanine catabolism braF lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-isoleucine catabolism natA lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-leucine catabolism natA lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-proline catabolism natA lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-serine catabolism braF lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-threonine catabolism braF lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-valine catabolism natA lo NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 92% 113.2 nodulation ATP-binding protein I 50% 310.8
L-arginine catabolism braG lo ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 33% 98% 110.2 nodulation ATP-binding protein I 50% 310.8
L-glutamate catabolism braG lo ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 33% 98% 110.2 nodulation ATP-binding protein I 50% 310.8
L-histidine catabolism braG lo ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 33% 98% 110.2 nodulation ATP-binding protein I 50% 310.8
L-valine catabolism livF lo ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 33% 98% 110.2 nodulation ATP-binding protein I 50% 310.8
L-alanine catabolism braG lo High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 31% 91% 109.4 nodulation ATP-binding protein I 50% 310.8
L-histidine catabolism natA lo NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 32% 90% 109.4 nodulation ATP-binding protein I 50% 310.8
L-serine catabolism braG lo High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 31% 91% 109.4 nodulation ATP-binding protein I 50% 310.8
L-threonine catabolism braG lo High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 31% 91% 109.4 nodulation ATP-binding protein I 50% 310.8
D-lactate catabolism PGA1_c12640 lo D-lactate transporter, ATP-binding component (characterized) 31% 93% 100.9 nodulation ATP-binding protein I 50% 310.8

Sequence Analysis Tools

View WP_038018808.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSVISPSPVLQARGLYKRFGGTTVVDRIDLDIRPGECFGILGPNGAGKTTTMRMMLGLSP
MDGGSLEIFGLPMPAAAERVRARLGVVPQMDNLDPDFTVAENLRIYGKYFGLEAAVLAER
IGKLLDFVELADRADTRIATLSGGMKRRLTIARALVNEPELVLLDEPTTGLDPQARHVIW
QRLRALRQAGKTLVLTTHYMEEAERLCDRLVIMDHGRILAEGSPQALIAAHVPAEVVEMR
SKYGGHMPPLDQLPAHLYESREVAGDTVFFYVRDAEPLLEAMHRLPDIITLNRPANLEDV
FLKLTGRDLRD

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory