Alignments for a candidate for liuA in Sulfuritalea hydrogenivorans DSM 22779

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuA in Sulfuritalea hydrogenivorans DSM 22779

Align Putative acyl-CoA dehydrogenase AidB; EC 1.3.99.- (characterized)
to candidate WP_041096225.1 SUTH_RS00425 isovaleryl-CoA dehydrogenase

Query= SwissProt::P33224
         (541 letters)



>NCBI__GCF_000828635.1:WP_041096225.1
          Length = 551

 Score =  491 bits (1264), Expect = e-143
 Identities = 254/509 (49%), Positives = 335/509 (65%), Gaps = 4/509 (0%)

Query: 5   THTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLANV 64
           TH V NQ   L N N YL++  L EAV ++GAGW  D+L + G + G+AE +E GRLAN 
Sbjct: 11  THEVLNQAHALENYNAYLNNVPLQEAVAQQGAGWAQDWLIARGAETGSAEWIEHGRLANA 70

Query: 65  NPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFMLH 124
           NPP+L  +D  G R D+V FHP+WH  +  L  +      W  D + GA VARAA +++ 
Sbjct: 71  NPPQLRLFDRYGNRRDEVEFHPSWHECLGWLKRHGCDTGPWA-DPKPGAHVARAAAYVMF 129

Query: 125 AQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLIGMGM 184
           A++E GSLCP TMT+ A P+L + +P   +DW   LLS  YD   +P  QK G+LIGMG+
Sbjct: 130 AEIEDGSLCPTTMTYGAVPVL-KHVPEIARDWMPKLLSRDYDKRFIPAAQKTGVLIGMGL 188

Query: 185 TEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFL 244
           TEKQGGSDV +NTTRAER++DGS+R+ GHKWF S P  DA LV AQ+  GLSCFFVPR+ 
Sbjct: 189 TEKQGGSDVRANTTRAERMDDGSWRITGHKWFLSAPMCDAFLVTAQSPKGLSCFFVPRWT 248

Query: 245 PDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRFDCAL 304
           PDG+ N +R++R K+K+G+RSNA  E EF  + GWL+G EG GI  +L+MG  TR DCA+
Sbjct: 249 PDGRLNELRIQRFKEKMGDRSNAGTEAEFWGSQGWLVGEEGRGIPTVLEMGVYTRLDCAI 308

Query: 305 GSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLARAWDR 364
           GS  +MR A S A++H   R  FG  L  QPLM + L+ +AL+ E  TAL  RLARA+D 
Sbjct: 309 GSTGIMRGALSQAMHHVGLRSAFGKLLRDQPLMINTLADLALETEAATALSMRLARAFDA 368

Query: 365 RADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPVNSIW 424
           + D  E L  RL TP AKF ICKR    VAEAMEV GG GY +E  +PRL+++ P+NSIW
Sbjct: 369 QEDESENLLRRLLTPVAKFWICKRCPAMVAEAMEVHGGNGYVDEGPMPRLFKQSPLNSIW 428

Query: 425 EGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKP--AEELG 482
           EGSGN+MCLD LR + +     D+L+       G++R FD    +L+  L  P   E  G
Sbjct: 429 EGSGNVMCLDTLRAMGRHPKSVDVLAAEIAPALGKNRAFDAFAAKLKDGLANPQDIETRG 488

Query: 483 REITHQLFLLGCGAQMLKYASPPMAQAWC 511
           RE+T  + L   GA +L++A  P+++A+C
Sbjct: 489 RELTQGIALAMQGALLLRHAPAPVSEAFC 517


Lambda     K      H
   0.324    0.138    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 721
Number of extensions: 36
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 541
Length of database: 551
Length adjustment: 35
Effective length of query: 506
Effective length of database: 516
Effective search space:   261096
Effective search space used:   261096
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources