Alignments for a candidate for liuA in Sulfuritalea hydrogenivorans DSM 22779

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuA in Sulfuritalea hydrogenivorans DSM 22779

Align Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (characterized)
to candidate WP_041096878.1 SUTH_RS02630 isovaleryl-CoA dehydrogenase

Query= reanno::Smeli:SM_b21121
         (387 letters)



>NCBI__GCF_000828635.1:WP_041096878.1
          Length = 388

 Score =  587 bits (1512), Expect = e-172
 Identities = 295/385 (76%), Positives = 326/385 (84%), Gaps = 3/385 (0%)

Query: 5   GLNFALGEEIDALRASVRRFASERIAPLADDADRSNAFPMSLWREMGELGLLGITADEAH 64
           G+NF LGE++D LR +V +FA + I P A + DR N FP  LW++ G++GLLG+TADE +
Sbjct: 3   GINFDLGEDVDMLRDAVWQFAQKEILPRAAEIDRDNLFPADLWKKFGDMGLLGMTADEEY 62

Query: 65  GGAGLGYLAHCVAMEEISRASASVGLSYGAHSNLCVNQINRNGKPAQKSRYLPKLISGEH 124
           GG  +GYLAH VAMEEISRASASVGLSYGAHSNLCVNQI RNG  AQK++YLPKLISGEH
Sbjct: 63  GGTKMGYLAHIVAMEEISRASASVGLSYGAHSNLCVNQIRRNGNEAQKAKYLPKLISGEH 122

Query: 125 VGALAMSEPGAGSDVVSMKLKADKRGDRYVLNGSKMWITNGPDADVLVVYAKTDPAAGPR 184
           VGALAMSEPGAGSDVVSMKL+AD +GDRYVLNGSKMWITNG DAD LVVYAKT P AG R
Sbjct: 123 VGALAMSEPGAGSDVVSMKLRADLKGDRYVLNGSKMWITNGGDADTLVVYAKTSPDAGAR 182

Query: 185 GITAFLVEKAFPGFSAGQKLDKLGMRGSNTSELIFTDCEVPEENVLGGVGEGVKVLMSGL 244
           G+TAF+VEK F GFS GQ LDKLGMRGSNT  L F DCEVP ENV+GG G G +VLMSGL
Sbjct: 183 GMTAFIVEKGFAGFSKGQHLDKLGMRGSNTYPLFFDDCEVPAENVMGGEGNGTRVLMSGL 242

Query: 245 DYERVVLSAGPLGIMAACLDVVVPYLHERKQFGQPIGEFQLMQGKLADMYVTMNAARAYV 304
           DYER VLS GPLGIMAAC+DVV+P++HERKQFGQ IGEFQLMQGKLADMY T  A RAYV
Sbjct: 243 DYERAVLSGGPLGIMAACMDVVLPFIHERKQFGQSIGEFQLMQGKLADMYSTWQATRAYV 302

Query: 305 YAVAAACDRGE---TARKDAAGCILYAAEKATAMALEAIQALGGNGYTNDYPAGRLLRDA 361
           YA+  ACDRG+   T RKDAAG ILY+AEKAT MA EAIQALGG GYTN+YPAGRL RDA
Sbjct: 303 YALGKACDRGDHARTLRKDAAGAILYSAEKATWMAGEAIQALGGVGYTNEYPAGRLWRDA 362

Query: 362 KLYEIGAGTSEIRRMLIGRELFAET 386
           KLYEIGAGTSEIRRMLIGREL+ ET
Sbjct: 363 KLYEIGAGTSEIRRMLIGRELYNET 387


Lambda     K      H
   0.318    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 491
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 387
Length of database: 388
Length adjustment: 30
Effective length of query: 357
Effective length of database: 358
Effective search space:   127806
Effective search space used:   127806
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources