Alignments for a candidate for ech in Sulfuritalea hydrogenivorans DSM 22779

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Sulfuritalea hydrogenivorans DSM 22779

Align Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate WP_041097743.1 SUTH_RS05635 3-hydroxyacyl-CoA dehydrogenase

Query= SwissProt::Q08426
         (723 letters)



>NCBI__GCF_000828635.1:WP_041097743.1
          Length = 697

 Score =  509 bits (1312), Expect = e-148
 Identities = 301/711 (42%), Positives = 425/711 (59%), Gaps = 35/711 (4%)

Query: 6   RLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGADIRG 65
           +LH + A+I L NPPVN +   L   I  G+ +A  D  +KA+V+ G++  FS GADIR 
Sbjct: 7   QLHGSTAVITLDNPPVNGLGYDLRCGIVAGIDQATADPAVKAVVLIGSDRAFSGGADIRE 66

Query: 66  FSAPRTFGL-TLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQVGLPE 124
           F +P+++    L  V+  ++   KPVVAAI G+  GGGLEL LGCH+R+A A A + LPE
Sbjct: 67  FGSPKSYAEPNLLTVIRIVEACPKPVVAAIGGVCMGGGLELTLGCHFRVAIAGASIALPE 126

Query: 125 VTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNS---DPVEEA 181
           + LGLLPGA GTQ +PR+ GV AAL+LI SG  + +++     + D+ +     D ++ A
Sbjct: 127 IKLGLLPGAGGTQRMPRIVGVEAALNLILSGNPVPSEKFKGTPLFDEFIEGNGGDLLKGA 186

Query: 182 IRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAV 241
           + FA +V  +    +R+ +  I   P  ++ F  +   ++       A   C+ AVQAA+
Sbjct: 187 LAFADKVVAEKRPLKRIRDIRID-YPGQEAFFQFSRNMVKGVAGPFPAPFKCIDAVQAAL 245

Query: 242 QYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTPSGASWKTASARPVSSVG 301
             P++ G+K E   F+ L+ + ++RAL++AF  ER A+K       +     AR ++ VG
Sbjct: 246 TKPFDEGMKFERACFIELMGTPESRALRHAFTGERAASKIPDVPDDT----PARKIARVG 301

Query: 302 VVGLGTMGRGIVISFARARIPVIAVDSDKNQL----ATANKMITSVLEK---EASKMQQS 354
           V+G GTMG GI ++F  A IPV  ++  +  L    AT  K   + ++K      K+ Q 
Sbjct: 302 VIGAGTMGGGITMNFLNAGIPVTMLEMKQEALDKGIATIRKNYENSMKKGKLTQEKLDQR 361

Query: 355 GHPWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALD 414
               SG     T S  +    DL+IEAVFEE+S+K+ VF +L A+ KP A L +NTS LD
Sbjct: 362 MGMLSG-----TLSYDDFKDADLIIEAVFEEISVKEAVFKQLDAIAKPGAILASNTSTLD 416

Query: 415 VDEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVG 474
           V++IA+ T RP  V+G HFFSPA+VMKLLEV+    ++   +ATVM ++KKIKK  VV G
Sbjct: 417 VNKIANFTKRPQDVVGMHFFSPANVMKLLEVVRGAATAKDVMATVMQVAKKIKKTAVVSG 476

Query: 475 NCFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSR 534
            C GF+GNRM+  Y   A FLLEEG+ P +VD+ LE++G  MGPFR+ DLAG D+GW  R
Sbjct: 477 VCDGFIGNRMIEHYGRVAGFLLEEGATPAQVDKALEKWGMAMGPFRMGDLAGNDIGWAIR 536

Query: 535 KGQGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDP 594
           K + +  P++            RY    D LCE GR+GQKTGKGWY Y +P  R   PDP
Sbjct: 537 KRRYVEKPSI------------RYAKFADKLCEQGRYGQKTGKGWYLY-QPGNRKAIPDP 583

Query: 595 WLSKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGY 654
            +   L  YRK   I PR IS  EI++RC+Y+L+NE  RIL EGIAA    ID++YL GY
Sbjct: 584 EVDTMLEAYRKELGITPRKISDAEIVQRCIYALVNEGARILEEGIAARASDIDMIYLTGY 643

Query: 655 GWPRHKGGPMFYASTVGLPTVLEKLQKYYRQNPDIPQLEPSDYLKKLASQG 705
           G+P H+GGPM YA  VGL  V   ++++   + D    EP+  + K  + G
Sbjct: 644 GFPLHRGGPMLYADEVGLYNVARSMKEFAATSGD-AFWEPAPLIAKRVADG 693


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1170
Number of extensions: 58
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 723
Length of database: 697
Length adjustment: 39
Effective length of query: 684
Effective length of database: 658
Effective search space:   450072
Effective search space used:   450072
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources