Align 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized)
to candidate WP_041097743.1 SUTH_RS05635 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein
Query= BRENDA::A4YDS4 (651 letters) >NCBI__GCF_000828635.1:WP_041097743.1 Length = 697 Score = 189 bits (481), Expect = 3e-52 Identities = 133/399 (33%), Positives = 207/399 (51%), Gaps = 26/399 (6%) Query: 2 KVTVIGSGVMGHGIAELAAIAGNEVWMNDISTEILQQAMERIKWSLSKLRESGSL-KEGV 60 +V VIG+G MG GI AG V M ++ E L + + I+ + + G L +E + Sbjct: 299 RVGVIGAGTMGGGITMNFLNAGIPVTMLEMKQEALDKGIATIRKNYENSMKKGKLTQEKL 358 Query: 61 EQVLARIHPETDQAQALKGSDFVIEAVKEDLELKRTIFRNAEAHASPSAVLATNTSSLPI 120 +Q + + T K +D +IEAV E++ +K +F+ +A A P A+LA+NTS+L + Sbjct: 359 DQRMGMLSG-TLSYDDFKDADLIIEAVFEEISVKEAVFKQLDAIAKPGAILASNTSTLDV 417 Query: 121 SEIASVLKSPQRVVGMHFFNPPVLMPLVEIVRGKDTSDEVVKTTAEMAKSMNKETIVVKD 180 ++IA+ K PQ VVGMHFF+P +M L+E+VRG T+ +V+ T ++AK + K +V Sbjct: 418 NKIANFTKRPQDVVGMHFFSPANVMKLLEVVRGAATAKDVMATVMQVAKKIKKTAVVSGV 477 Query: 181 VPGFFVNRVLLRIMEAGCYLVEKGIASIQEVDSSAIEELGFPMGVFLLADYTGLDIGYSV 240 GF NR++ +L+E+G A+ +VD A+E+ G MG F + D G DIG+++ Sbjct: 478 CDGFIGNRMIEHYGRVAGFLLEEG-ATPAQVD-KALEKWGMAMGPFRMGDLAGNDIGWAI 535 Query: 241 WKAVTARGFKAFPCSSTEKLVSQGKLGVKSGSGYYQYPS------PGKFVRPTLPSTSKK 294 K +KL QG+ G K+G G+Y Y P V L + K+ Sbjct: 536 RKRRYVEKPSIRYAKFADKLCEQGRYGQKTGKGWYLYQPGNRKAIPDPEVDTMLEAYRKE 595 Query: 295 LG------------RYLISPAVNEVSYLLREGIVGK-DDAEKGCVLGLGLP---KGILSY 338 LG + I VNE + +L EGI + D + + G G P G + Y Sbjct: 596 LGITPRKISDAEIVQRCIYALVNEGARILEEGIAARASDIDMIYLTGYGFPLHRGGPMLY 655 Query: 339 ADEIGIDVVVNTLEEMRQTSGMDHYSPDPLLLSMVKEGK 377 ADE+G+ V +++E TSG + P PL+ V +GK Sbjct: 656 ADEVGLYNVARSMKEFAATSGDAFWEPAPLIAKRVADGK 694 Score = 99.4 bits (246), Expect = 5e-25 Identities = 67/193 (34%), Positives = 95/193 (49%), Gaps = 9/193 (4%) Query: 408 AWIVLNRPTRYNAINGDMIREINQALDSLEEREDVRVIAITGQGRVFSAGADVTEFGSLT 467 A I L+ P N + D+ I +D V+ + + G R FS GAD+ EFGS Sbjct: 13 AVITLDNPP-VNGLGYDLRCGIVAGIDQATADPAVKAVVLIGSDRAFSGGADIREFGS-- 69 Query: 468 PVKAMIASRKFHEVFMKIQFLTKPVIAVINGLALGGGMELALSADFRVASKTAEMGQPEI 527 A V ++ KPV+A I G+ +GGG+EL L FRVA A + PEI Sbjct: 70 --PKSYAEPNLLTVIRIVEACPKPVVAAIGGVCMGGGLELTLGCHFRVAIAGASIALPEI 127 Query: 528 NLGLIPGGGGTQRLSRLSG-RKGLELVLTGRRVKAEEAYRLGIVEFLAE---PEELESEV 583 LGL+PG GGTQR+ R+ G L L+L+G V +E+ + + E + L+ + Sbjct: 128 KLGLLPGAGGTQRMPRIVGVEAALNLILSGNPVPSEKFKGTPLFDEFIEGNGGDLLKGAL 187 Query: 584 RKLANAIAEKSPL 596 +AEK PL Sbjct: 188 AFADKVVAEKRPL 200 Lambda K H 0.316 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 959 Number of extensions: 46 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 2 Length of query: 651 Length of database: 697 Length adjustment: 39 Effective length of query: 612 Effective length of database: 658 Effective search space: 402696 Effective search space used: 402696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory