Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_041097743.1 SUTH_RS05635 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein
Query= reanno::BFirm:BPHYT_RS13545 (706 letters) >NCBI__GCF_000828635.1:WP_041097743.1 Length = 697 Score = 632 bits (1630), Expect = 0.0 Identities = 325/690 (47%), Positives = 459/690 (66%), Gaps = 9/690 (1%) Query: 13 ELRGKVLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAGADIRE 72 +L G ++T+D+ PVN L D+R G++A I+ A AD AV+AV+++G+ R F GADIRE Sbjct: 7 QLHGSTAVITLDNPPVNGLGYDLRCGIVAGIDQATADPAVKAVVLIGSDRAFSGGADIRE 66 Query: 73 FGKPP--VPPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLGLPE 130 FG P P+L V +EAC KPVVAAI G +GGGLE+ L H+R+A+ GA + LPE Sbjct: 67 FGSPKSYAEPNLLTVIRIVEACPKPVVAAIGGVCMGGGLELTLGCHFRVAIAGASIALPE 126 Query: 131 VQLGLLPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRL--GSSDDILAEG 188 ++LGLLPGAGGTQR PR++G +AAL+LILSG +++ L D G+ D+L Sbjct: 127 IKLGLLPGAGGTQRMPRIVGVEAALNLILSGNPVPSEKFKGTPLFDEFIEGNGGDLLKGA 186 Query: 189 LAYVHELLAAHAPVRRTRDAAALSDRAASLAAVATARAETAKKSRGLFSPLKIVDAVEAA 248 LA+ +++A P++R RD D A +R + +P K +DAV+AA Sbjct: 187 LAFADKVVAEKRPLKRIRDIRI--DYPGQEAFFQFSRNMVKGVAGPFPAPFKCIDAVQAA 244 Query: 249 IEQPFDEGLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAAKP-RTLNTIGVVG 307 + +PFDEG++ ER F+E + +P+ L HAF ER K P+ P R + +GV+G Sbjct: 245 LTKPFDEGMKFERACFIELMGTPESRALRHAFTGERAASKIPDVPDDTPARKIARVGVIG 304 Query: 308 GGTMGAGIAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALMSR 367 GTMG GI + L+AG+PVTM+E +L +G A I K Y+ + KG+L+ EK M Sbjct: 305 AGTMGGGITMNFLNAGIPVTMLEMKQEALDKGIATIRKNYENSMKKGKLTQEKLDQRMGM 364 Query: 368 WSGSTSYDALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALASSV 427 SG+ SYD ADL+IEAVFE+++VK+AVF +LD + K GA+LA+NTS LD++ +A+ Sbjct: 365 LSGTLSYDDFKDADLIIEAVFEEISVKEAVFKQLDAIAKPGAILASNTSTLDVNKIANFT 424 Query: 428 SRPADVIGLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGN 487 RP DV+G+HFFSPAN+MKLLEVV + DV+AT ++AKK++KT V +GVCDGFIGN Sbjct: 425 KRPQDVVGMHFFSPANVMKLLEVVRGAATAKDVMATVMQVAKKIKKTAVVSGVCDGFIGN 484 Query: 488 RVLAVYRSAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAATRN 547 R++ Y A ++E+GA+P Q+D A+ +G MGPF++ DLAG DIGWA RKRR + Sbjct: 485 RMIEHYGRVAGFLLEEGATPAQVDKALEKWGMAMGPFRMGDLAGNDIGWAIRKRRYVEK- 543 Query: 548 PAARYVQIADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPRSF 607 P+ RY + AD+LCE+G +GQK+G+G+YLY G+R PDPEV+ +++A R GITPR Sbjct: 544 PSIRYAKFADKLCEQGRYGQKTGKGWYLYQPGNRKAIPDPEVDTMLEAYRKELGITPRKI 603 Query: 608 TDEEIIRRYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYADMVGLPK 667 +D EI++R + A++NEGA ++ E IA R D+D+ +L GYGFP +RGGPM YAD VGL Sbjct: 604 SDAEIVQRCIYALVNEGARILEEGIAARASDIDMIYLTGYGFPLHRGGPMLYADEVGLYN 663 Query: 668 ILADIREF-AKEDPLFWKPSPLLIELVERG 696 + ++EF A FW+P+PL+ + V G Sbjct: 664 VARSMKEFAATSGDAFWEPAPLIAKRVADG 693 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1106 Number of extensions: 48 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 697 Length adjustment: 39 Effective length of query: 667 Effective length of database: 658 Effective search space: 438886 Effective search space used: 438886 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory