Align Putative enoyl-CoA hydratase protein; EC 4.2.1.17 (characterized, see rationale)
to candidate WP_041098567.1 SUTH_RS08545 enoyl-CoA hydratase
Query= uniprot:Q92VJ6 (261 letters) >NCBI__GCF_000828635.1:WP_041098567.1 Length = 264 Score = 140 bits (352), Expect = 4e-38 Identities = 82/253 (32%), Positives = 132/253 (52%), Gaps = 8/253 (3%) Query: 11 DQRGVARVTLARSEKHNALSATMIGELTAVVGRLATDASIRAVILDAEGKSFCAGGDLDW 70 D+ GVA +TL R + N+LS M+GEL A + +A D ++RAV++ GK+FCAG DL Sbjct: 15 DREGVATLTLNRPTQFNSLSEEMLGELQAALNAIAADKTVRAVVIAGAGKAFCAGHDLKQ 74 Query: 71 MRQQFSADRPTRIAEATRLAMMLKALNDLPKPLIARVHGNAFGGGVGLISVCDTVIAASG 130 MR S ++ + MM ++ ++P+P+IAR+HG A G L+++CD +AA G Sbjct: 75 MRANHSKTYMQKLFKQCGKVMM--SIVEMPQPVIARIHGIATAAGCQLVAMCDLAVAAEG 132 Query: 131 AQFGLTETRLGLIPATISPYVIARTGEARARPLFMSARVFGAEEAKVAGFVTTVVDGTML 190 A+F ++ +GL +T S + G A + ++ A EA+ G + VV L Sbjct: 133 ARFAVSGINVGLFCSTPSVALGRAMGRKEAMEMLLTGDFIDAAEAQRRGLINRVVPLEQL 192 Query: 191 DGAVEAAVTAYLVAAPGAAGRAKRL---ARSLGLPITDAVIAATIEQLADTWETDEAREG 247 D V+ + + A G K++ +GL D +E +A D+A EG Sbjct: 193 DAEVKKLTDSICAKSAVAVGMGKQMFYKQLEMGL---DGAYQLAVETMACNMMADDAAEG 249 Query: 248 VSAFFERRNPSWR 260 + AF ++R P W+ Sbjct: 250 IDAFMQKRKPEWK 262 Lambda K H 0.321 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 157 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 261 Length of database: 264 Length adjustment: 25 Effective length of query: 236 Effective length of database: 239 Effective search space: 56404 Effective search space used: 56404 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory