Alignments for a candidate for adh in Sulfuritalea hydrogenivorans DSM 22779

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Sulfuritalea hydrogenivorans DSM 22779

Align 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 (characterized)
to candidate WP_041099671.1 SUTH_RS12545 CoA-acylating methylmalonate-semialdehyde dehydrogenase

Query= SwissProt::P56533
         (503 letters)



>NCBI__GCF_000828635.1:WP_041099671.1
          Length = 505

 Score =  254 bits (648), Expect = 7e-72
 Identities = 161/484 (33%), Positives = 239/484 (49%), Gaps = 16/484 (3%)

Query: 19  DDLNYWGGRRIKSKDGATTEPVFEPATGRVLCQMVPCGAEEVDQAVQSAQAAYLKWSKMA 78
           D + +W      +  G     VF PA GRV  Q+      +VD+AV +A+AA+  W   A
Sbjct: 10  DLVGHWINGHADAGAGTRVGEVFNPAEGRVARQVALATRADVDRAVAAARAAFADWGSAA 69

Query: 79  GIERSRVMLEAARIIRERRDNIAKLEVINNGKTITEAEYDIDAAWQCIEYYAGLAPTLSG 138
              R+RVM +   ++ +   +IAKL    +GKT+ +A  ++    + IE+  G+   L G
Sbjct: 70  PQRRARVMFKFRDLVDQYSTDIAKLLSAEHGKTLPDALGEVQRGLEVIEFACGIPQLLKG 129

Query: 139 QHIQ-LPGGAFAYTRREPLGVCAGILAWNYPFMIAAWKCAPALACGNAVVFKPSPMTPVT 197
           QH   + GG   + +R PLGV AGI  +N+PFM+  W    ALACGN  + KPS   P  
Sbjct: 130 QHSHGIGGGIDHWNQRMPLGVTAGITPFNFPFMVPMWMAPMALACGNTFILKPSERDPSP 189

Query: 198 GVILAEIFHEAGVPVGLVNVVQGGAETGSLLCHHPNVAKVSFTGSVPTGKKVMEMSAKTV 257
            +  AE+  EAG+P G  NV+QG  E    L  HP V+ VSF GS P  K +        
Sbjct: 190 SLFAAELLREAGLPAGAFNVIQGDKEAVDALLEHPFVSAVSFVGSTPIAKYIQAHGIAHG 249

Query: 258 KHVTLELGGKSPLLIFKDCELENAVRGALMANFLTQGQVCTNGTRVFVQREIMPQFLEEV 317
           K V    G K+ +++  D +L+ A    + A + + G+ C   +            +  V
Sbjct: 250 KRVQALGGAKNHMVVMPDADLDGAADALIGAAYGSAGERCMAISVAVAVGSCADDLVARV 309

Query: 318 VKRTKAIVVGDPLLTETRMGGLISKPQLDKVLGFVAQAKKEGAR-VLCGGEPLTPSDPKL 376
           + R K + VG        MG L++    DKV G++A   +EGA+ VL G E LT    +L
Sbjct: 310 LARAKKLKVGAGDAANVEMGPLVTAVHRDKVAGYIAAGVQEGAKLVLDGREGLT---GEL 366

Query: 377 KNGYFMSPCVLDNCRDDMTCVKEEIFGPVMSVLPFDTEEEVLQRANNTTFGLASGVFTRD 436
             G+F++P + DN   +M+  +EEIFGPV+ V+   T    ++  N  TF      FTRD
Sbjct: 367 AQGFFINPTLFDNVTPNMSIYQEEIFGPVLCVVRVPTFAAAVELINRHTFANGVACFTRD 426

Query: 437 ISRAHRVAANLEAGTCYINTYSISPVEVP-----FGGYKMSGFGREN--GQATVDYYSQL 489
              A      +E G   IN     P+ VP     FGG+K S FG  +  G+  V +Y++ 
Sbjct: 427 GGTAQAFIQKIEVGMVGINV----PIPVPMAWHSFGGWKASLFGDHHIYGEEGVRFYTRY 482

Query: 490 KTVI 493
           K ++
Sbjct: 483 KAIM 486


Lambda     K      H
   0.319    0.135    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 578
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 503
Length of database: 505
Length adjustment: 34
Effective length of query: 469
Effective length of database: 471
Effective search space:   220899
Effective search space used:   220899
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources