Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_041578178.1 XAUT_RS25450 methylmalonyl-CoA mutase
Query= BRENDA::O74009 (563 letters) >NCBI__GCF_000017645.1:WP_041578178.1 Length = 560 Score = 582 bits (1501), Expect = e-170 Identities = 289/556 (51%), Positives = 394/556 (70%), Gaps = 3/556 (0%) Query: 9 LKKIKEEEKRWEETTVKKFLEKAPERKEKFMTDDGFEIKRIYTPADLGEDWNYMEKLGFP 68 L + ++ + WEET V FL+K ERKE+F T F +KR+YT AD +E +G P Sbjct: 7 LANLDDDVRHWEETEVAAFLKKQKERKEQFFTLGDFPVKRVYTAADAAA--TPIEDIGLP 64 Query: 69 GEYPFTRGVYATMYRGRIWTMRQYAGYATAEESNKRYKYLLSQGQTGLSVAFDLPTQLGY 128 G YPFTRG Y TMYR R WTMRQ AG+ T E++NKR+KYL+ QGQTG+S FD+PT +GY Sbjct: 65 GRYPFTRGPYPTMYRSRNWTMRQIAGFGTGEDTNKRFKYLIEQGQTGISTDFDMPTLMGY 124 Query: 129 DSDHPLAEGEVGKVGVAIDSLWDMRILFDGIPLDKVSTSMTINSTAANLLAMYILVAEEQ 188 DSDHP+++GEVG+ GVAID+L DM LFDGI L+K+S SMTIN +A LLAMYI++A+++ Sbjct: 125 DSDHPMSDGEVGREGVAIDTLADMEALFDGIDLEKISVSMTINPSAWILLAMYIVLAQKR 184 Query: 189 GVSQEKLRGTVQNDILKEYIARGTYIFPPQPSMRLTTDIIMYCAENVPKWNPISISGYHI 248 G +KL GTVQ DILKEY+A+ YI+P PS+R+ D I YCA+N+ ++NPI+ISGYHI Sbjct: 185 GYDLDKLSGTVQADILKEYMAQKEYIYPIAPSVRIVRDCITYCAKNMKRYNPINISGYHI 244 Query: 249 REAGANAVQEVAFTLADGIEYVKAVIERGMDVDKFAPRLSFFFAAHNNFLEEIAKFRAAR 308 EAG++ V EVAFTLA+ I YV+ V++ GM VD FAPRL+FFF +F EEIAKFRA R Sbjct: 245 SEAGSSPVDEVAFTLANLIVYVEEVLKTGMKVDDFAPRLAFFFVCQADFFEEIAKFRAVR 304 Query: 309 RLWAYIMKEWFNAKNPRSMMLRFHTQTAGSTLTAQQPENNIVRVAIQALAAVLGGTQSLH 368 R +A IMKE F A+NP SM LRFH QTA ++LT Q N+VR +QALAAVLGG QSLH Sbjct: 305 RCYAKIMKERFGARNPESMRLRFHCQTAAASLTKPQFMVNVVRTTLQALAAVLGGCQSLH 364 Query: 369 TNSYDEALSLPTEKSVRIALRTQQIIAYESGVVDTVDPLGGAYYIEWLTDHIYEEALKYI 428 TN +DEA ++PTE+++R+ALRTQQ+IA ES V +DP+GG+YY+E LT + + I Sbjct: 365 TNGFDEAFAIPTEEAMRLALRTQQVIAEESNVTQVIDPVGGSYYVETLTTEYEKRIMDII 424 Query: 429 EKIQKMGGMMRAIERGYVQKEIAEAAYKYQKEIEEGKRIIVGVNAFVTDEPI-EVEILKV 487 ++ GG ++ I+ G+ QK +A+ AY+ + G++ ++GVN V + + E+E+ Sbjct: 425 SEVDARGGTIKLIQEGWFQKSVADFAYETALRKQSGEKPVIGVNTMVDESEVHEIELHPY 484 Query: 488 DPSIREKQIERLKKLRSERDNKKVQEALDKLRNAAEKEDENLMPYIIEAHRHLATLQEVT 547 D + ++QI R +++R ERDN KV LD+L A+ E +N+MP IE R AT+ ++ Sbjct: 485 DHTTADRQIARTQRVRRERDNVKVSALLDRLVEVAKDETQNIMPVTIELVREGATMGDIV 544 Query: 548 DVLREIWGEYRAPLIF 563 + L+ +WG YR +F Sbjct: 545 ERLKTVWGVYRETPVF 560 Lambda K H 0.318 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 793 Number of extensions: 35 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 563 Length of database: 560 Length adjustment: 36 Effective length of query: 527 Effective length of database: 524 Effective search space: 276148 Effective search space used: 276148 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory