Alignments for a candidate for PS417_12065 in Rhizobium leguminosarum 3841

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_12065 in Rhizobium leguminosarum 3841

Align D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_041936920.1 RL_RS31840 sugar ABC transporter ATP-binding protein

Query= uniprot:A0A1N7TX47
         (495 letters)



>NCBI__GCF_000009265.1:WP_041936920.1
          Length = 511

 Score =  360 bits (923), Expect = e-104
 Identities = 200/492 (40%), Positives = 297/492 (60%), Gaps = 1/492 (0%)

Query: 4   PLLLQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSI 63
           P +L+   +++ + GV AL +  ++L  G+V AL G NGAGKST + IL GI + + G I
Sbjct: 18  PAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEI 77

Query: 64  LLNGAPVQFNRPSEALAAGIAMITQELEPIPYMTVAENIWLGREPRRAGCIVDNKALNRR 123
           L++G PV F     A+ AG+  I QE      +TVAENI+LG  PR     +D +A+N R
Sbjct: 78  LVDGQPVTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRLRTIDWQAMNSR 137

Query: 124 TRELLDSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQTLF 183
            + LL +LE ++D T  +   S+AQ  LV IA+A S + +++IMDEPT+A+   E   LF
Sbjct: 138 AKALLTALESNIDPTIRLKDFSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDDLF 197

Query: 184 KAIRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIVGQE 243
           + +R L  +G  I+++SH+  E+ +IADD+ +FRDG  V  GR+ +  +D +VR +VG++
Sbjct: 198 RIVRGLKEKGKAILFISHKFDEVYEIADDFVVFRDGRAVGQGRLKETPQDEIVRMMVGRD 257

Query: 244 LTRIDHKVGRECAANTCLQVDNLSRAGEFHDISLQLRQGEILGIYGLMGSGRSEFLNCIY 303
           +     KV         L++ N S   EF DIS  LRQGEILGIYGL+G+GRSE    ++
Sbjct: 258 VENAFPKVD-VAFGGPVLEIRNYSHRTEFRDISFTLRQGEILGIYGLIGAGRSELSQSLF 316

Query: 304 GLTVADSGSVTLQGKPMPIGLPKATINAGMSLVTEDRKDSGLVLTGSILSNIALSAYKRL 363
           G+T   SG + L+G+ + I  P+  I AG+  V E+R   GL L   I  N+ L +  R 
Sbjct: 317 GITRPLSGKMMLEGREITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLTRT 376

Query: 364 SSWSLINARKETQLAEDMVKRLQIKTTSLELPVASMSGGNQQKVVLAKCLSTEPVCLLCD 423
           S    + A +E  LA    +RL ++  +L +PV ++SGGNQQKVV+ K L+T P  ++ D
Sbjct: 377 SRRGFLRAAEEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVIILD 436

Query: 424 EPTRGIDEGAKQEIYHLLDQFVRGGGAAIVVSSEAPELLHLSDRIAVFKGGRLVTISTDT 483
           EPT+GID G+K  ++  + +    G + I+VSSE PE++ +SDR+ V K G    I    
Sbjct: 437 EPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLAAGIFERA 496

Query: 484 ALSQEALLRLAS 495
            LS EAL+R A+
Sbjct: 497 ELSPEALVRAAT 508



 Score = 78.2 bits (191), Expect = 6e-19
 Identities = 66/233 (28%), Positives = 102/233 (43%), Gaps = 9/233 (3%)

Query: 16  YAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILLNGAPVQFNRP 75
           Y+     RD   +LR G +  + G  GAG+S     L GIT+  +G ++L G  +  + P
Sbjct: 279 YSHRTEFRDISFTLRQGEILGIYGLIGAGRSELSQSLFGITRPLSGKMMLEGREITIHSP 338

Query: 76  SEALAAGIAMITQELE------PIPYMTVAENIWLGREPRRAGC-IVDNKALNRRTRELL 128
            +A+ AGI  + +E        P+P         L R  RR      +  AL R+  E L
Sbjct: 339 QDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLTRTSRRGFLRAAEEFALARKYAERL 398

Query: 129 DSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQTLFKAIRR 188
           D     +    P+  LS    Q V I K  +   +V+I+DEPT  I       +   I  
Sbjct: 399 DLRAAALSV--PVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISE 456

Query: 189 LTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIVG 241
           L A+G  I+ VS  L E+  ++D   + ++G        A++  + LVR   G
Sbjct: 457 LAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLAAGIFERAELSPEALVRAATG 509


Lambda     K      H
   0.319    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 553
Number of extensions: 22
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 495
Length of database: 511
Length adjustment: 34
Effective length of query: 461
Effective length of database: 477
Effective search space:   219897
Effective search space used:   219897
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources