Align Branched-chain amino acid ABC transporter,substrate-binding periplasmic component (characterized, see rationale)
to candidate WP_042446662.1 AZL_RS31475 branched-chain amino acid ABC transporter substrate-binding protein
Query= uniprot:G8ALJ3 (366 letters) >NCBI__GCF_000010725.1:WP_042446662.1 Length = 366 Score = 660 bits (1704), Expect = 0.0 Identities = 327/366 (89%), Positives = 346/366 (94%) Query: 1 MNYKLSLLVAVAATAMTASVAKADIAVATAGPITGQYATFGEQMKKGIEQAVADINAAGG 60 M +KLSLL A+AATA++A+ AKADIAVATAGPITGQYATFGEQMKKG+EQAVADINAAGG Sbjct: 1 MKFKLSLLAAIAATALSATAAKADIAVATAGPITGQYATFGEQMKKGMEQAVADINAAGG 60 Query: 61 VLGQKLKLEVGDDACDPKQAVAVANQLAKAGVKFVAGHFCSGSSIPASQVYAEEGVLQIS 120 VLGQKLKLEVGDDACDPKQAVAVANQLAKAGVKFVAGHFCSGSSIPASQVY+EEG+LQIS Sbjct: 61 VLGQKLKLEVGDDACDPKQAVAVANQLAKAGVKFVAGHFCSGSSIPASQVYSEEGILQIS 120 Query: 121 PASTNPKLTEQNLKNVFRVCGRDDQQGQIAGKYLLENYKGKNVAILHDKSAYGKGLADET 180 PASTNPKLTEQ LKNVFRVCGRDDQQG IAGKYLL+ YKGKNVAILHDKSAYGKGLADET Sbjct: 121 PASTNPKLTEQGLKNVFRVCGRDDQQGAIAGKYLLDTYKGKNVAILHDKSAYGKGLADET 180 Query: 181 QKALNAGGQKEKIYEAYTAGEKDYSALVSKLKQEAVDVVYVGGYHTEAGLLARQMKDQGL 240 QKALNAGGQKE IYEAYTAGEKDYSALVSKLKQ VD VY+GGYHTEAGL+ARQMKDQGL Sbjct: 181 QKALNAGGQKETIYEAYTAGEKDYSALVSKLKQANVDAVYIGGYHTEAGLIARQMKDQGL 240 Query: 241 NAPIVSGDALVTNEYWAITGPAGENTMMTFGPDPREMPEAKEAVEKFRKAGYEPEGYTLY 300 A IVSGDALVTNEYWAITG AGE+TMMTFGPDPRE P+AK VEKFRKAGYEPEGYTLY Sbjct: 241 KAIIVSGDALVTNEYWAITGDAGEDTMMTFGPDPREKPDAKAVVEKFRKAGYEPEGYTLY 300 Query: 301 TYAALQIWAEAAKQANSTDSAKIADVLRKNSYNTVIGKIGFDAKGDVTSPAYVWYRWNNG 360 TYAALQIWA A KQANSTD+AK+ADVLRK SY+TVIGKIGFDAKGDVTSPAYVWY+W+ G Sbjct: 301 TYAALQIWANAVKQANSTDAAKVADVLRKGSYDTVIGKIGFDAKGDVTSPAYVWYKWHAG 360 Query: 361 QYAQVK 366 QYA+VK Sbjct: 361 QYAEVK 366 Lambda K H 0.312 0.129 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 605 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 366 Length of database: 366 Length adjustment: 30 Effective length of query: 336 Effective length of database: 336 Effective search space: 112896 Effective search space used: 112896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory